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Full-Text Articles in Analytical Chemistry
Synthesis And Evaluation Of Novel Silica Hydride-Based Stationary Phases For Bioanalytical Applications
Seiichiro Watanabe
Most HPLC columns are packed with silanol (Si-OH)-rich type-B silica that often
participates in undesired electrostatic interactions with sample solutes and produces a poor analyte peak shape. These silanols are also known to facilitate the surface hydration required for HILIC mode to retain polar analytes. However, the hydrated surface composition can easily fluctuate, which results in poor reproducibility and requires a lengthy equilibration step. In this study, four novel stationary phases have been developed by using the TYPE-CTM silica material, in which up to 95% of the surface silanols have been replaced with Si-H group. One of the advantages of …