Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Metabolomics (2)
- Quorum Sensing (2)
- Acylhomoserine lactone (1)
- Antibody (1)
- Autoinducer 2 (1)
-
- Autoinducer-2 (1)
- Bacterial Membranes (1)
- Cytokine (1)
- Daptomycin (1)
- E. faecalis (1)
- Exosomes (1)
- FM Probes (1)
- FTIR (1)
- Germination (1)
- Immunosensor (1)
- Inactivation (1)
- Marine Microbial Metabolism (1)
- Non-thermal plasma (1)
- Nonlinear Spectroscopy (1)
- Pentose phosphate pathway (1)
- Protein A (1)
- Quartz Crystal Microbalance (1)
- Riboneogenesis (1)
- SHG (1)
- Spore (1)
Articles 1 - 5 of 5
Full-Text Articles in Analytical Chemistry
Using Second Harmonic Generation To Study Gram-Positive Bacterial Membranes, Lindsey N. Miller
Using Second Harmonic Generation To Study Gram-Positive Bacterial Membranes, Lindsey N. Miller
Doctoral Dissertations
Understanding how small-molecules, such as drugs, interact with bacterial membranes can quickly unravel into much more perplexing questions. No two bacterial species are alike, especially when comparing their membrane compositions which can even be altered by incorporating fatty acids from their surrounding environment into their lipid-membrane composition. To further complicate the comparison, discrete alterations in small-molecule structures can result in vastly different membrane-interaction outcomes, giving rise to the need for more "label-free" studies when analyzing drug mechanisms. The work presented in this dissertation highlights the benefits to using nonlinear spectroscopy and microscopy techniques for probing small-molecule interactions in living bacteria. …
Design And Development Of A Quartz Crystal Microbalance Immunosensor For Exosomes, Wesley Cox
Design And Development Of A Quartz Crystal Microbalance Immunosensor For Exosomes, Wesley Cox
Chancellor’s Honors Program Projects
No abstract provided.
Quorum Sensing And Metabolism In Marine Environments, Amanda May
Quorum Sensing And Metabolism In Marine Environments, Amanda May
Doctoral Dissertations
Quorum sensing (QS) is a phenomenon that allows bacteria to communicate with each other. Small molecules known as autoinducers are synthesized and released by bacteria, and once enough members of the community are around to ensure survival, i.e. quorum, a phenotype, e.g. bioluminescence, is expressed. There are two types of QS molecules, intra- and inter-species.
S-4,5-Dihydroxy-2,3-pentanedione (DPD) is a byproduct of the activated methyl cycle which recycles methionine. This has led to the discussion as to whether DPD is a metabolic byproduct or is the interspecies signal as proposed previously. The detection and quantitation of DPD however, has not …
Development And Application Of Liquid Chromatography-Tandem Mass Spectrometry Methods To The Understanding Of Metabolism And Cell-Cell Signaling In Several Biological Systems, Jessica Renee Gooding
Development And Application Of Liquid Chromatography-Tandem Mass Spectrometry Methods To The Understanding Of Metabolism And Cell-Cell Signaling In Several Biological Systems, Jessica Renee Gooding
Doctoral Dissertations
Liquid chromatography tandem mass spectrometry has become a powerful tool for investigating biological systems. Herein we describe the development of both isotope dilution mass spectrometry methods and targeted metabolomics methods for the study of metabolic and cell-cell signaling applications.
A putative yeast enzyme was characterized by discovery metabolite profiling, kinetic flux profiling, transcriptomics and structural biology. These experiments demonstrated that the enzyme shb17 was a sedoheptulose bisphosphatase that provides a thermodynamically dedicated step towards riboneogenesis, leading to the redefinition of the canonical pentose phosphate pathway.
An extension of metabolic profiling and kinetic flux profiling methods was developed for a set …
Non-Thermal Plasma Inactivation Of Bacillus Amyloliquefaciens Spores, Yaohua Huang
Non-Thermal Plasma Inactivation Of Bacillus Amyloliquefaciens Spores, Yaohua Huang
Masters Theses
Bacterial spores have remarkable resistance to a variety of harsh conditions, causing spoilage in food industry and becoming the primary bacterial agent in biowarfare and bioterrorism. In this study, inactivation mechanisms of Bacillus amyloliquefaciens (BA) spores by non-thermal plasma (NTP) were investigated by using Fourier-transform infrared spectroscopy (FTIR) as a major tool to exam spores after NTP treatment. Chemometric techniques, such as multivariate classification models based on soft independent modeling of Class Analogy (SIMCA) and Principal Component Analysis (PCA), were employed to identify functional group changes in FTIR spectra. The IR absorbance bands correlated to dipicolinic acid (DPA) decreased after …