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Direct Haplotyping Of Kilobase-Size Dna Using Carbon Nanotube Probes, Adam T. Woolley, Chantal Guillemette, Chin Li Cheung, David E. Housman, Charles M. Lieber
Direct Haplotyping Of Kilobase-Size Dna Using Carbon Nanotube Probes, Adam T. Woolley, Chantal Guillemette, Chin Li Cheung, David E. Housman, Charles M. Lieber
Barry Chin Li Cheung Publications
We have implemented a method for multiplexed detection of polymorphic sites and direct determination of haplotypes in 10-kilobasesize DNA fragments using single-walled carbon nanotube (SWNT) atomic force microscopy (AFM) probes. Labeled oligonucleotides are hybridized specifically to complementary target sequences in template DNA, and the positions of the tagged sequences are detected by direct SWNT tip imaging. We demonstrated this concept by detecting streptavidin and IRD800 labels at two different sequences in M13mp18. Our approach also permits haplotype determination from simple visual inspection of AFM images of individual DNA molecules, which we have done on UGT1A7, a gene under study as …