Physical Sciences and Mathematics Commons^™

Development Of A Novel Lysophosphatidic Acid Activity Probe To Identify And Characterize New Protein Targets, Leah M. Cuthriell

Masters Theses

Lipids are categorized according to their biological function. Phospholipids, glycolipids, and cholesterol are bulk membrane lipids that offer structure and support to the cells and organelle membranes. Signaling lipids include diacylglycerol, phosphatidic acid, and lysophosphatidic acid (LPA). These are low abundance lipids that are active in various pathways. LPA acts through GPCR receptors to activate G or beta mediated stimulation of phospholipase C leading to phosphatidylinositol-(4,5)-bisphosphate hydrolysis, G_i mediated inhibition of adenyl cyclase, and G_i mediated stimulation of the mitogenic Ras- MAP kinase. To further elucidate the biological activity of LPA and potentially identify new LPA binding receptors, …

The Impact Of Iron Deprivation On The Metabolism Of Escherichia Coli As Measured By Targeted Hplc-Ms/Ms Based Metabolomics, Jamie Rhea Light

Masters Theses

Iron is a necessity for all living organisms, and bacteria typically need an internal concentration of iron in the micromolar range.¹ This becomes quite problematic, especially in pathogenic bacteria, because the concentration of free ions within the human body is only 10^-24 Molar due to iron binding proteins, like transferrin.¹ In order to circumvent this problem, bacteria produce small iron chelating molecules called siderophores.¹ The bacteria utilized for this study, a K-12 derivative of Escherichia coli, produce only one siderophore, enterobactin. Not only is the transcription of genes associated with iron acquisition initiated in response …

An Exploration Of Monophonic Instrument Classification Using Multi-Threaded Artificial Neural Networks, Marc Joseph Rubin

Masters Theses

The use of computers for automated music analysis could benefit several aspects of academia and industry, from psychological and music research, to intelligent music selection and music copyright investigation. In the following thesis, one of the first steps of automated musical analysis, i.e., monophonic instrument recognition, was explored. A multi-threaded artificial neural network was implemented and used as the classifier in order to utilize multi-core technology and allow for faster training. The parallelized batch-mode backpropagation algorithm used provided linear speedup, an improvement to the current literature. For the classification experiments, eleven different sets of instruments were used, starting with perceptively …

Phosphorus Transport From A Field Receiving Long-Term Liquid Dairy Manure Application In A Karst Landscape, Dustin Cody Graham

Masters Theses

Agricultural runoff is a leading non-point source contributor to water quality impairment in the United States and is associated with eutrophication of surface waters. Phosphorus (P) is often the most limiting nutrient for eutrophication in freshwaters. The objectives of this study were to characterize the P forms in surface runoff from an agricultural field that has received long-term applications of liquid dairy manure and to determine the forms of soil P that occur within a sinkhole feature located within the application field. Three 21-m x 6 m bermed plots were established to collect storm water runoff from a portion of …

Design And Synthesis Of Fret-Based Boronic Acid Receptors To Detect Carbohydrate Clustering And Development Of Diacylglycerol-Based Lipid Probesto Investigate Lipid-Protein Binding Interactions, Manpreet Kaur Cheema

Masters Theses

Carbohydrate–protein interactions play vital roles in various biochemical processes such as signal transduction and cell surface recognition events. The clustering of carbohydrates into dense domains such as lipid rafts regulates recognition by multivalent receptors (i.e. lectins). These regions are known to play important roles in biological processes such as cellular transduction and trafficking. In order to characterize the clustering of glycans on cell surfaces, detection of domains with high carbohydrate density is of great interest. In this thesis, we present the work based on a modular strategy to design and synthesize boronic acid-based carbohydrate receptors, which are termed as boronolectins …

An Investigation Into The Activities Of 1,2,3 & 1,2,4 Triazoles In Biological And Catalytic Systems, David John Schneider

Masters Theses

Selective epigenetic control of the cellular machinery is a grail of drug development. The balance between HAT’s and HDAC’s activities is one way the cell controls what, and at when during the cellular lifecycle a gene is expressed. Having the ability to lock chromatin down to the histone is a powerful tool for the treatment of disease states like cancer. Presented here is a preliminary exploration of the synthesis, and basic testing of novel compounds that use a triazole motif as a zinc binding group in an attempt to gain further selectivity in inhibition over HDAC’s.

Interest in the development …

Polarimetry Studies For The Npdgamma Experiment At The Sns, Mostafa Jon Dadras

Masters Theses

The NPDGamma collaboration aims to measure a parity{violating directional gamma-ray asymmetry from the capture of polarized cold neutrons on protons. The asymmetry, predicted to be on the order of 50 ppb, is a result of the weak interaction between mesons and nucleons during the neutron capture reaction. The experiment is designed to make a statistics limited measurement of the asymmetry at the level of about 20%. The experiment will be carried out at the Fundamental neutron Physics Beamline (FnPB) at the Spallation Neutron Source (SNS). The neutron beam is polarized by a super{mirror polarizer. A 9.5 G magnetic field guides …

Accelerated Ctis Using The Cell Processor, Thaddeus James Thompson

Masters Theses

The Computed Tomography Imaging Spectrometer (CTIS) is a device capable of simultaneously acquiring imagery from multiple bands of the electromagnetic spectrum. Due to the method of data collection from this system, a processing intensive reconstruction phase is required to resolve the image output. This paper evaluates a parallelized implementation of the Vose-Horton CTIS reconstruction algorithm using the Cell processor. In addition to demonstrating the feasibility of a mixed precision implementation, it is shown that use of the parallel processing capabilities of the Cell may provide a significant reduction in reconstruction time.

A Statistical Analysis Of Key Factors Influencing The Location Of Biomass-Using Facilities, Xu Liu

Masters Theses

Bioenergy and biofuels are emerging industries in the U.S. economy that will require statistical and economical analyses of woody biomass resources, supply chains, and other key factors that influence the siting of industrial facilities. This thesis develops models using logistic regression to improve the understanding of the key factors that influence the locations of existing wood-using bioenergy and biofuels plants, and other wood-using plants. The scope of the study is 13 Southeastern states.¹ Logistic regression models are developed at the state and regional levels. The resolution of the study is the ZIP Code tabulation area (ZCTA). There are 9,416 …

Application Of The Explicit Asymptotic Method To Nuclear Burning In Type Ia Supernova, Christopher Ryan Smith

Masters Theses

Modern problems in astrophysics tend to require large, complex computational frameworks to solve many aspects of the system simultaneusly. Calculation of the energy production through nuclear reactions is typically one of those aspects. The use of standard nuclear burning algorithms will take up the majority of the computational time with all but the smallest of networks. The explicit asymptotic method has shown promise in computing large networks faster than existing methods in various environments while retaining accuracy. The purpose of this thesis is to show that this method can be successfully used to solve complex systems using a network of …

Fluorescence Characterization Of Karst Aquifers In East Tennessee, Teresa L. Brown

Masters Theses

Karst and fractured rock aquifers are primary sources of drinking water in the Appalachian region, even though most are inherently susceptible to surface-derived contamination. Many of the obstacles to water supply protection in such systems could be alleviated through the use of tracer testing to delineate recharge areas and surface-to-groundwater connections. Tracer testing is currently under-utilized, however, due to public safety concerns and ambivalence on the part of regulatory agencies. This study aimed to address this issue through a characterization of the fluorescent properties of typical carbonate aquifers for the purpose of refining the timing and design of low-concentration dye …

Single Molecule Detection Of Near-Infrared Phthalocyanine Dyes, You Li

Masters Theses

The major advantage associated with near-infrared monitoring is the fact that few compounds show intrinsic fluorescence in this region of the spectrum and hence background from other molecules is reduced. Phthalocyanine dyes provide emission at deep red and near infrared wavelengths but have excellent photostability and hence are an attractive candidate for near-infrared fluorescence bioassay applications. However, because of their small Stokes shifts, non-standard methods are needed for optimum separation of fluorescence from scattered laser light. This thesis reports the development of a custom confocal microscope that uses a lowcost laser diode operating at 667 nm for sample excitation and …

Elemental Analysis Of Soils Using Laser-Induced Breakdown Spectroscopy (Libs), Ningfang Yang

Masters Theses

Laser-induced breakdown spectroscopy (LIBS) has been applied for the quantitative analysis of various samples, but it is controversial for chemically-complex soils. In order to study the effect of delay time, gate width, and repetition rate on the LIBS signal in soil, the emission line at 396.84 nanometer was selected. The results indicated that repetition rate, delay time, and gate width significantly affected the LIBS signal, delay time was more important than gate width in controlling the LIBS signal. The optimum instrumental parameters for soil analysis were obtained when repetition rate, delay time, and gate width equaled 10 hertz, 1 microsecond, …

Some Congruence Modulo 2 Statements Of Primitive Conway Vassiliev Invariants., James M. Dawson

Masters Theses

Polynomial knot invariants can often be used to define Vassiliev invariants on singu- lar knots. Here Vassiliev invariants form the Conway, Jones, HOMFLY, and Kauffman polynomials are explored. Also, some explanation is given about how symbols of the Jones and Conway polynomial can evaluated on suitable chord diagrams. These in- variants are further used to find expressions that are congruent modulo 2 to some low degree invariants derived from the Primitive Conway polynomial.

Geometrical Effects On Electromagnetic Enhancement To Sers From Metal Nanoparticle Dimer Arrays, Abigail Laurel Gaddis

Masters Theses

Much recent effort has been devoted to the study of the optical properties of metal nanoparticle substrates. In such finely ordered structures, surface plasmons can be induced by incident light. These collective excitations of the electrons in the nanoparticle create localized areas of high electromagnetic field intensity. The intense local fields generated are of interest for various applications, including Surface-enhanced Raman Scattering (SERS) for molecular detection and sensing. In this thesis, the optical properties of various nanoparticle dimer and array geometries are considered, including gold triangular prism dimers and silver spherical dimers. The effect of SERS due to electromagnetic interaction …

High-Sensitivity Spectral Fluorescence Lifetime Imaging For Resolving Spectroscopically Overlapping Species, Justin Lee Crawford

Masters Theses

The capability to resolve the contributions from spectroscopically overlapping fluorophores has enabled significant breakthroughs in cellular imaging. However, commercial microscopes for this purpose use analog light detection with least squares curve-fitting analysis and improvements in sensitivity are needed. To this end, a microscope has been constructed with high throughput and single-photon detection capability. The fluorescence is separated through use of a prism spectrometer or a series of dichroic mirrors into four spectral bands and detected using four single-photon avalanche diode (SPAD) detectors, which provide high-quantum efficiency in the red spectral region. The detectors are connected to a time-correlated single photon …

An Automated Approach To The Study And Classification Of Colliding And Interacting Galaxies, Steven Alvaro Boada

Masters Theses

Colliding galaxies are perhaps the greatest events changing and evolving our Universe. Consequently, the need for an understanding of how that interaction originated is very important. This thesis presents a framework in which the study of these events can be conducted in a timely and efficient manner. A genetic algorithm coupled with an initial conditions generator, a physics engine and an analysis package performs an automated search to visually match an unknown galactic interaction with a known event, thus providing the starting conditions that created such an interaction.

Microfluidic Device For The Electrokinetic Manipulation Of Single Molecules, Jason Keith King

Masters Theses

Manipulation and trapping of single molecules in solution is a useful tool for studies of biological molecules and molecular dynamics, but has to date been implemented in only one or two dimensions. The goal of the research in this thesis is to develop a microfluidic device to address the need for three-dimensional manipulation of single fluorescent molecules in solution. The device consists of two pairs of platinum electrodes deposited onto two standard borosilicate glass coverslips via ultraviolet microlithography and ionic sputtering. The two coverslips are sandwiched together such that the electrode tips form a tetrahedral configuration, and by applying appropriate, …

Oxidative Stress, Calcium Homeostasis, And Altered Gene Expression In Human Lung Epithelial Cells Exposed To Zno Nanoparticles, Chuan-Chin Huang

Masters Theses

"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 μg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. Exposure to a sublethal concentration of ZnO …

Information Flow Properties For Cyber-Physical Systems, Rav Akella

Masters Theses

"In cyber-physical systems, which are the integrations of computational and physical processes, security properties are difficult to enforce. Fundamentally, physically observable behavior leads to violations of confidentiality. This work analyzes certain noninterference based security properties to ensure that interactions between the cyber and physical processes preserve confidentiality. A considerable barrier to this analysis is the representation of physical system interactions at the cyber-level. This thesis presents encoding of these physical system properties into a discrete event system and represents the cyber-physical system using Security Process Algebra (SPA). The model checker, Checker of Persistent Security (CoPS) shows Bisimulation based NonDeducibility on …

Stability Of Heme Proteins: Cytochrome C And Horseradish Peroxidase, Mini Gupta

Masters Theses

No abstract provided.

Electrochemical And Spectroscopic Characterization Of A Polypyridyl Ru(Ii) Complex Containing A Fused Alloxazine, Waynie Wilson Olaprath

Masters Theses

No abstract provided.

Formation Of Iron-Rimmed Sandstone Nodules On Earth; Terrestrial Analogue For The Formation Of Martian Blueberries?, Katherine Charlotte Muller

Masters Theses

"NASA's twin Mars Exploration Rovers, MER, Spirit and Opportunity, have identified numerous geologic features that hint at a watery past on Mars noted in Malik, 2004. Prominent among these features are the "Martian Blueberries" that occur as spherical hematite (Fe₂O₃) nodules. Hematite formation is facilitated by the presence of water, although there are presently no known sources of free liquid water on Mars. Chan et al., 2004 states that these "blueberries" are also similar to nodules found in the Jurassic aged Navajo Sandstone in Utah. An understanding of the processes controlling the formation of the Utah nodules may thus be …

Satellite Photography: Instrumental, Rhetorical, Affective?, Irangi Kaushalya Egodapitiya

Masters Theses

"This study examines how tsunami satellite images operate and could provoke various interpretations. The goal of this research project is to study whether the interpretation of tsunami satellite images could extend beyond scientific features to affective messages. Primarily based on the satellite images of Sri Lanka taken before and during the 2004 tsunami, this study analyses: How could mechanical satellite images, taken automatically by a machine in orbit, become an object of profound emotional meaning?; How do viewer's experiences, knowledge, and discourse affect their interpretations of mechanical artifacts?; How are tsunami satellite images connected to affective responses by viewers?; How …

The Analogue Of The Iterated Logarithm For Quantum Difference Equations, Karl Friedrich Ulrich

Masters Theses

"In this thesis, we consider oscillation and nonoscillation of q-difference equations, i.e., equations that arise while studying q-calculus. In particular, we prove an extension of Kneser’s theorem on q-calculus to cases in which no conclusion can be drawn by applying Kneser’s theorem. In order to accomplish this, we establish a change of variables which yields, when applied iteratively, a sequence of comparison functions. We use these comparison functions to establish our main result. Finally, we consider an analogue result for time scales which are unbounded from above"--Abstract, page iii.

Environmental Geochemistry Of Metal Contaminated Sediments From The Big River System Of Southeastern Missouri, Helen Carrie Bender

Masters Theses

"Geochemical analysis of stream sediments from the Big River Watershed of southeastern Missouri indicates that they contain elevated concentrations of contaminant metals such as Pb. Zn, Cu. Co, Ni and Cd. The elevated concentrations are derived from natural exposures of metal enriched strata and the numerous mine tailings piles and water seeps created as a result of about 300 years of lead-zinc mining in the “Old Lead Belt”. Galena (PbS), sphalerite (ZnS), and pyrite (FeS₂) are the primary sulfides found in the tailings piles and Scanning Electron Microscopy analysis of individual metallic sediment particles collected at intervals downstream …

Exploring The Use Of A Commercial Game Engine For The Development Of Educational Software, Hussain Alafaireet

Masters Theses

"Three-dimensional visualization and simulation environments are becoming increasingly important in both industrial and educational environments. However, developing these simulations can be a difficult and expensive task. Herein we explore the use of a commercial game engine to develop a 3-D, interactive computer game designed to teach players about the anatomy of a frog. The goal of this project is threefold: 1) to develop high-fidelity virtual reality educational software with a limited budget and limited personnel, 2) to define a software development process by which similar simulations can be developed, and 3) to explore the benefits that these simulations provide to …

Integration Of A Gis Learning System Into Civil Engineering Curricula: An Evaluation, Basanta Tandon

Masters Theses

"An evaluation of a web-based e-learning system to facilitate integration of Geographical Information Systems (GIS) into the Civil Engineering curriculum was conducted. The principal goals of the evaluation were to examine the effectiveness of the learning system and to develop a preliminary model to describe how students interact with the learning system. Quantitative and qualitative data were collected from 80 students who participated in a geotechnical engineering laboratory session, which covered soil borrow sites. Students rated the laboratory session as significantly more effective for learning, a nd more motivational than the class text. They also rated the lab significantly more …

In Vitro Evaluation Of Bioactive Glass Scaffolds And Modified Bioactive Glasses With An Osteogenic Cell Line, Vernon C. Modglin

Masters Theses

"This investigation consisted of two parts focused on bioactive glasses and scaffolds for in vitro growth of bone cells for tissue engineering and bone repair. The first part involved evaluation of the ability of two new types of porous 13-93 bioactive glass scaffolds to support the growth and differentiation of mouse MLO-AS cells, an established osteogenic cell line used as a model of developing bone tissue. The two scaffold types tested included 13-93 glass fiber and trabecular-like scaffolds seeded with MLO-AS cells and cultured for intervals of 2 to 12 days. Collectively, in vitro results indicate that the 13-93 glass …

The Effects Of Technology On Literacy(S), Thomas Glen Petersen

Masters Theses

"One of the challenges when measuring the effects of technology on literacy(s) is finding a control group where technology; does not play a major role in the culture. The Amish successfully live in a Technocracy where mechanical/electronic tools are used only when absolutely necessary; the Amish are not dependent on them for survival. The crux of the Amish philosophy is humility, serving the community and serving God. Ironically, they have suffered tremendous repercussions for adopting a wholesome, selfless lifestyle which is contrary to most popular world views, regardless of religious or spiritual views"--Purpose, page 40.