Physical Sciences and Mathematics Commons^™

Development Of A Novel Lysophosphatidic Acid Activity Probe To Identify And Characterize New Protein Targets, Leah M. Cuthriell

Masters Theses

Lipids are categorized according to their biological function. Phospholipids, glycolipids, and cholesterol are bulk membrane lipids that offer structure and support to the cells and organelle membranes. Signaling lipids include diacylglycerol, phosphatidic acid, and lysophosphatidic acid (LPA). These are low abundance lipids that are active in various pathways. LPA acts through GPCR receptors to activate G or beta mediated stimulation of phospholipase C leading to phosphatidylinositol-(4,5)-bisphosphate hydrolysis, G_i mediated inhibition of adenyl cyclase, and G_i mediated stimulation of the mitogenic Ras- MAP kinase. To further elucidate the biological activity of LPA and potentially identify new LPA binding receptors, …

An Investigation Into The Activities Of 1,2,3 & 1,2,4 Triazoles In Biological And Catalytic Systems, David John Schneider

Masters Theses

Selective epigenetic control of the cellular machinery is a grail of drug development. The balance between HAT’s and HDAC’s activities is one way the cell controls what, and at when during the cellular lifecycle a gene is expressed. Having the ability to lock chromatin down to the histone is a powerful tool for the treatment of disease states like cancer. Presented here is a preliminary exploration of the synthesis, and basic testing of novel compounds that use a triazole motif as a zinc binding group in an attempt to gain further selectivity in inhibition over HDAC’s.

Interest in the development …

The Impact Of Iron Deprivation On The Metabolism Of Escherichia Coli As Measured By Targeted Hplc-Ms/Ms Based Metabolomics, Jamie Rhea Light

Masters Theses

Iron is a necessity for all living organisms, and bacteria typically need an internal concentration of iron in the micromolar range.¹ This becomes quite problematic, especially in pathogenic bacteria, because the concentration of free ions within the human body is only 10^-24 Molar due to iron binding proteins, like transferrin.¹ In order to circumvent this problem, bacteria produce small iron chelating molecules called siderophores.¹ The bacteria utilized for this study, a K-12 derivative of Escherichia coli, produce only one siderophore, enterobactin. Not only is the transcription of genes associated with iron acquisition initiated in response …

Design And Synthesis Of Fret-Based Boronic Acid Receptors To Detect Carbohydrate Clustering And Development Of Diacylglycerol-Based Lipid Probesto Investigate Lipid-Protein Binding Interactions, Manpreet Kaur Cheema

Masters Theses

Carbohydrate–protein interactions play vital roles in various biochemical processes such as signal transduction and cell surface recognition events. The clustering of carbohydrates into dense domains such as lipid rafts regulates recognition by multivalent receptors (i.e. lectins). These regions are known to play important roles in biological processes such as cellular transduction and trafficking. In order to characterize the clustering of glycans on cell surfaces, detection of domains with high carbohydrate density is of great interest. In this thesis, we present the work based on a modular strategy to design and synthesize boronic acid-based carbohydrate receptors, which are termed as boronolectins …

Molecular Structures And Pulsed Discharge Emission Studies Of Volatile Organic Compound Derivatives, Ashley Osthoff

Masters Theses

No abstract provided.

Cloning, Overexpression, Purification And Characterization Of Gcp Ii Homologous Metalloprotein From Caulobacter Crescentus, Dilani Nilushika Gigiriwala Gamage

Masters Theses

No abstract provided.

Electrochemical And Spectroscopic Characterization Of A Polypyridyl Ru(Ii) Complex Containing A Fused Alloxazine, Waynie Wilson Olaprath

Masters Theses

No abstract provided.

Stability Of Heme Proteins: Cytochrome C And Horseradish Peroxidase, Mini Gupta

Masters Theses

No abstract provided.