Veterinary Infectious Diseases Commons^™

Evaluation Of Six Serological Elisa Kits Available In Italy As Screening Tests For Equine Infectious Anaemia Surveillance, Roberto Nardini, Gian Luca Autorino, Charles J. Issel, Richard F. Cook, Ida Ricci, Raffaele Frontoso, Francesca Rosone, Maria Teresa Scicluna

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). For this purpose, an “in-house” and five commercial ELISAs available in Italy were assessed by the National Reference Centre for EIA for their analytic specificity (Sp); precocity, defined as capability of detecting first antibodies produced during a new infection; precision based on repeatability and reproducibility, estimated from the coefficient of variation (CV); accuracy, estimated from multiple K and relative Sp and sensitivity (Se). Two serum panels, positive for non-equine retroviruses and the most frequent …