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Veterinary Infectious Diseases Commons™
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- Keyword
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- PEDV (10)
- Swine (8)
- Feed matrix (3)
- PCR (3)
- Bovine respiratory disease (2)
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- Chemical treatment (2)
- Feed (2)
- Medium chain fatty acids (2)
- Bioassay (1)
- Cattle (1)
- Chemical sanitation (1)
- Decontamination (1)
- Enterotoxigenic Escherichia coli (ETEC) (1)
- Epitopes (1)
- FaeG (1)
- Fat source (1)
- Feed additive (1)
- Feed manufacturing (1)
- Feed mill (1)
- Feed safety (1)
- Flush (1)
- High risk (1)
- K88 (1)
- Luminex (1)
- Medium chain fatty acid (1)
- Metaphylactic treatment (1)
- Minimum infectious dose (1)
- Molecular diagnostics (1)
- PCV2 (1)
- PCV3 (1)
Articles 1 - 14 of 14
Full-Text Articles in Veterinary Infectious Diseases
Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt
Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt
Kansas Agricultural Experiment Station Research Reports
The cost of Bovine Respiratory Disease to the beef industry due to death, poorer conversions, and therapy is estimated to cost more than $3 billion per year. Identifying and mitigating Bovine Respiratory Disease in cattle can be difficult due to the increased susceptibility for Bovine Respiratory Disease in high risk cattle. One management option to minimize an outbreak of respiratory disease is the use of metaphylaxis, the mass treatment of a group of calves to reduce the incidence and adverse effects of respiratory disease on high risk animals. Criteria used to determine the necessity of metaphylactic treatment against Bovine Respiratory …
Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang
Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang
Kansas Agricultural Experiment Station Research Reports
Enterotoxigenic Escherichia coli (ETEC) bacteria are the primary cause of diarrheal disease, especially porcine post-weaning diarrhea (PWD). Post-weaning diarrhea is one of the most common diseases in piglets 3 to 10 days after weaning and causes the loss of millions of dollars annually to United States swine industry and other countries. These ETEC bacteria produce two types of virulence factors: 1) fimbriae adhesins, which promote bacterial attachment and colonization in pig small intestine; and 2) enterotoxins that disrupt fluid homeostasis and cause fluid hype-secretion and watery diarrhea. The F4 (K88) is the most important fimbria in ETEC bacteria causing PWD. …
Zelnate On Arrival Could Decrease The Likelihood Of Subsequent Pulls In Suspect Bovine Respiratory Disease Complex Cases, T. Spore, S. P. Montgomery, G. A. Hanzlicek, W. R. Hollenbeck, R. N. Wahl, J. E. Sears, Dale Blasi
Zelnate On Arrival Could Decrease The Likelihood Of Subsequent Pulls In Suspect Bovine Respiratory Disease Complex Cases, T. Spore, S. P. Montgomery, G. A. Hanzlicek, W. R. Hollenbeck, R. N. Wahl, J. E. Sears, Dale Blasi
Kansas Agricultural Experiment Station Research Reports
Antimicrobial metaphylaxis is an important tool used for the prevention of Bovine Respiratory Disease Complex; a disease with a large economic impact that typically affects newly-weaned beef calves that are marketed and transported a distance from their origin.
There are questions involving the potential benefit of Zelnate, a novel non-antibiotic technology designed to activate an animal’s natural immunity to fight Bovine Respiratory Disease Complex, being used either solely or in combination with metaphylaxis at the time of initial processing of high risk calves. More knowledge is also needed regarding the possible effects of repeated use of Zelnate when subsequent therapy …
Genome Diversity And Molecular Detection Of Prrs Field Strains And Vaccine Strains, And Pcv3 And Pcv2 Strains, Y. Wang, F. Yuan, X. Liu, W. Zheng, H. Zhang, J. Zhang, K. Yoon, L. Peddireddi, Y. Fang, G. Anderson, J. Bai
Genome Diversity And Molecular Detection Of Prrs Field Strains And Vaccine Strains, And Pcv3 And Pcv2 Strains, Y. Wang, F. Yuan, X. Liu, W. Zheng, H. Zhang, J. Zhang, K. Yoon, L. Peddireddi, Y. Fang, G. Anderson, J. Bai
Kansas Agricultural Experiment Station Research Reports
Molecular diagnosis of porcine reproductive and respiratory syndrome virus (PRRS) and porcine circo virus (PCV) are challenging due to high genetic diversity in the viral genomes. Differentiating PRRS vaccine strains is even more challenging and is currently done by DNA sequencing, which is expensive and time-consuming. A multiplexed system (Luminex) allowing multiple detection targets in the same reaction is available. However, this system is not fully developed for common swine pathogens. Therefore, an assay was built to detect the majority of field PRRS strains by using different pairs of primers, and at the same time, to provide differentiation of the …
Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz
Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz
Kansas Agricultural Experiment Station Research Reports
Various strategies have been proposed to mitigate potential risk of porcine epidemic diarrhea virus (PEDV) transmission via feed and feed ingredients. Wet decontamination has been found to be the most effective decontamination of feed mill surfaces; however, this is not practical on a commercial feed production-scale. Another potential mitigation strategy, easier to implement, would be using chemically-treated rice hulls flushed through the feed manufacturing equipment. The objective of this experiment was to determine the impact of MCFA- or formaldehyde-treated rice hull flush batches as potential PEDV mitigation strategies during feed manufacturing. Feed without evidence of PEDV RNA contamination was inoculated …
Porcine Epidemic Diarrhea Virus Surface Decontamination Strategies Using Chemical Sanitizing To Reduce The Quantity Of Pedv Rna On Feed Manufacturing Surfaces With Environmental Swabbing, M. Muckey, S. S. Dritz, J. C. Woodworth, C. R. Stark, J. Bai, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones
Porcine Epidemic Diarrhea Virus Surface Decontamination Strategies Using Chemical Sanitizing To Reduce The Quantity Of Pedv Rna On Feed Manufacturing Surfaces With Environmental Swabbing, M. Muckey, S. S. Dritz, J. C. Woodworth, C. R. Stark, J. Bai, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Porcine Epidemic Diarrhea virus (PEDV) is a possible hazard in feed mills that could impact pig health. If the virus enters a feed mill, it quickly becomes widely distributed and is difficult to decontaminate from surfaces.6,7 The objective of this study was to evaluate a variety of liquid and dry chemical treatments that could be used as sanitizers to reduce the amount of PEDV found on feed manufacturing surfaces in mills. This experiment was replicated 3 times and was designed in a 5 × 10 factorial with main effects of 5 different feed manufacturing surfaces and 10 sanitizing treatments. Surfaces …
Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones
Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of individual MCFA levels are unknown. The objective of this study is to compare the efficacy of commercially-available sources of MCFA and other fat sources versus a synthetic custom blend of MCFA to minimize the risk of PEDV cross-contamination as measured by qRT-PCR and bioassay. Treatments were arranged in a 17 × 4 plus 1 factorial with 17 chemical treatments: 1) Positive control with PEDV …
Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones
Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of MCFA levels below 2% inclusion is unknown. The objective of this experiment was to evaluate if a 1% inclusion of MCFA is as effective at PEDV mitigation as a 2% inclusion or formaldehyde in swine feed and spray-dried animal plasma (SDAP). Treatments were arranged in a 4 × 2 × 7 plus 2 factorial with 4 chemical treatments: 1) PEDV positive with no chemical …
Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz
Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz
Kansas Agricultural Experiment Station Research Reports
Feed and feed ingredients have been shown to be potential vectors of porcine epidemic diarrhea virus (PEDV). Potential strategies to mitigate the risk of disease transmission via feed and feed ingredients would be valuable to the swine and feed milling industries. Therefore, the objective of this experiment was to determine the impact of VevoVitall (5,000 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), CRINA (200 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), and a combination of both products (COMBINATION; 5,000 ppm VevoVitall and 200 ppm CRINA) as feed additives with potential to mitigate the risk of PEDV, in swine gestation diet …
Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger
Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger
Kansas Agricultural Experiment Station Research Reports
This study aimed to utilize the only known pilot feed mill facility approved for pathogenic feed agent use in the United States to evaluate the effect of manufacturing Porcine Epidemic Diarrhea Virus (PEDV)-contaminated feed on subsequent feed mill environmental surface contamination. In this study, PEDV inoculated feed was manufactured and conveyed on equipment along with four subsequent batches of PEDV-free feed. Equipment and environmental surfaces were sampled using swabs and analyzed for the presence of PEDV RNA by PCR. The experiment was replicated three times with decontamination of the feed mill and all equipment between replications. Overall, environmental swabs indicated …
Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Kansas Agricultural Experiment Station Research Reports
Understanding the magnitude of transmissible risk Porcine Epidemic Diarrhea Virus (PEDV)-infected feed imposes and establishing the minimum infectious dose of PEDV in a feed matrix are important components in strengthening virus prevention and control methods. In this study, an experiment was performed involving 30 crossbred, 10-d-old pigs that were used as a bioassay model for the minimum infectious dose of PEDV in feed. The PEDV was first diluted using tissue culture media to form 8 serial 10-fold dilutions. An aliquot of the original stock virus at 5.6 x 105tissue culture infectious dose/ml (TCID50/ml), each serial PEDV …
Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones
Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. However, epidemiological evidence has shown that swine feed and ingredients may serve as potential vectors of transmission. Since it is known that PEDV is a heat-sensitive virus, we hypothesized that a conditioner and pellet mill mimicking commercial thermal processing would mitigate PEDV infectivity. To test this hypothesis, two experiments were designed to determine if different pellet mill conditioner retention times or temperatures would impact PEDV infectivity determined by polymerase chain reaction (PCR) analysis and bioassay. For the first study, a 3×3×2 factorial was utilized, with three pelleting temperatures (155, …
Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Kansas Agricultural Experiment Station Research Reports
Understanding key points of potential cross-contamination during the feed manufacturing process is important to developing efficacious methods to control or prevent transmission of pathogens into swine diets. In this study, an experiment was conducted involving 30 crossbred 10-d-old pigs that were used as a bioassay model for Porcine Epidemic Diarrhea Virus (PEDV) to determine the effects of feed batch sequencing on PEDV cross-contamination and subsequent infectivity. PEDV with a PCR cycle threshold value (Ct) of 11 was uniformly mixed into 4.5 kg of swine diet using a stainless steel bench top mixer validated for mixing efficiency. The inoculated feed was …
Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones
Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. Research has confirmed swine feed or ingredients as potential vectors of transmission, so strategies are needed to mitigate PEDV in feed. The objective of this experiment was to evaluate the effectiveness of various chemical additives to prevent or mitigate post-processing PEDV contamination in swine feed and ingredients. Treatments were arranged in a 7 × 4 factorial with seven chemical treatments and four feed matrices. The chemical treatments included: negative control with no chemical addition, 0.3% commercial formaldehyde product, 1% sodium bisulfate, 1% sodium chlorate, 3% custom organic acid blend …