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Full-Text Articles in Comparative and Laboratory Animal Medicine

Characterization Of Memory T-Lymphocyte Subsets In Dogs, Annie Beth Bauman Mar 2021

Characterization Of Memory T-Lymphocyte Subsets In Dogs, Annie Beth Bauman

LSU Master's Theses

A more complete understanding of canine T-cell immunity is necessary for improving diagnostic and therapeutic approaches to canine diseases, developing cell-based canine immunotherapeutics, and evaluating dogs as large mammal models for comparative immunology research. Canine immunological studies are limited by the lack of established phenotypic markers identifying diverse differentiation states in canine T-cells. Differential expression of CD45RA (indicating antigen experience) and CD62L (indicating lymph node homing capability), shows promise as a tool to delineate canine naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector memory (TEMRA) T-cells. The aim of this study was to characterize canine memory T-cell …


Preparation And Evaluation Of Single-Stranded Dna Aptamer-Based Immunological Adjuvant In Broiler Chickens, Adil Sabr Al-Ogaili May 2019

Preparation And Evaluation Of Single-Stranded Dna Aptamer-Based Immunological Adjuvant In Broiler Chickens, Adil Sabr Al-Ogaili

Graduate Theses and Dissertations

Mineral oils and metal salts are commonly used as adjuvants to enhance acquired immunity. Recently, monoclonal antibodies (MAbs) and recombinant peptides agonist CD40 receptor have shown remarkable promise for induction of rapid and robust immune responses. Limitations of this approach MAb production costs and multiple administrations due to anti-MAb immune responses. Here we demonstrate the development of a unique and sophisticated DNA aptamer-based alternative for CD40-directed delivery of universal antigens as an alternative in chickens, and potentially other vertebrate species. This receptor, expressed by antigen-presenting cells, acts as a costimulatory molecule for activated T helper lymphocytes. After initially selecting for …


Evaluación Molecular, De Ehrlichia Canis Y Babesia Canis En Caninos Militares De La Fuerza Aérea Colombiana, César Enrique Martínez Roa Jan 2019

Evaluación Molecular, De Ehrlichia Canis Y Babesia Canis En Caninos Militares De La Fuerza Aérea Colombiana, César Enrique Martínez Roa

Maestría en Ciencias Veterinarias

La Ehrlichiosis Monocítica Canina (EMC) y la Babesiosis Canina, son patologías de distribución mundial causadas por Ehrlichia canis y Babesia canis, respectivamente, comparten vector (Rhipicephalus sanguineus) y existen reportes en Colombia de infección individual y co-infección. La Fuerza Aérea Colombiana (FAC) cuenta con caninos entrenados en detecciones. En las Unidades de clima cálido del año 2008 al 2012, se ha reportado una alta tasa de mortalidad presuntivamente EMC, sin diagnóstico confirmado. Reemplazar un animal fallecido tiene altos costos económicos e intangibles (tiempo y capacidades adquiridas), por ello surge la necesidad de confirmar la posible presencia E. canis y B. canis …


The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio), Charles R. Wulff Jan 2011

The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio), Charles R. Wulff

Honors Theses

Anesthesia plays a vital role in the maintenance of aquaculture species, where it is used to minimize stress during complex handling tasks such as transport, assessment, and harvesting. However, anesthetics have been shown to suppress the innate immune response, which could impact immunity and increase risk of infection. Tricaine methanesulfonate (MS-222) and 2-Phenoxyethanol (2-PE) represent two of the most commonly used anesthetics in aquaculture, with R-(+)-carvone, in the form of carvone-methyl salicylate (CMS) has recently been proposed as an alternative anesthetic for food fish. These three anesthetics were used to assess the influence of anesthetics on the immune system of …