Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Ophthalmology
Colocalization Of Increased Transforming Growth Factor-Β-Induced Protein (Tgfbip) And Clusterin In Fuchs Endothelial Corneal Dystrophy, Ula V. Jurkunas, Maya Bitar, Ian Rawe
Colocalization Of Increased Transforming Growth Factor-Β-Induced Protein (Tgfbip) And Clusterin In Fuchs Endothelial Corneal Dystrophy, Ula V. Jurkunas, Maya Bitar, Ian Rawe
Maya S. Bitar
PURPOSE: To investigate the differential expression of TGFBIp in normal human and Fuchs endothelial corneal dystrophy (FECD) endothelial cell-Descemet’s membrane (HCEC-DM) complex, and to asses the structural role of TGFBIp and clusterin (CLU) in guttae formation. METHODS: HCEC-DM complex was dissected from stroma in normal and FECD samples. Proteins were separated by 2-D gel electrophoresis and subjected to proteomic analysis. N-terminal processing of TGFBIp was detected by Western blot analysis with two separate antibodies against the N- and C-terminal regions of TGFBIp. Expression of TGFBI mRNA was compared by using real-time PCR. Subcellular localization of TGFBIp and CLU in corneal …