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Full-Text Articles in Ophthalmology
Posterior Segment Approach For Subretinal Transplantation Or Injection In The Canine Model, Maria E. Verdugo, Julie Alling, Eliot Lazar, Manuel Del Cerro, Jharna Ray, Gustavo D. Aguirre
Posterior Segment Approach For Subretinal Transplantation Or Injection In The Canine Model, Maria E. Verdugo, Julie Alling, Eliot Lazar, Manuel Del Cerro, Jharna Ray, Gustavo D. Aguirre
Gustavo D. Aguirre, VMD, PhD
A posterior segment approach for cell transplantation or injection into the subretinal space of the dog has been developed. Controlled penetration to the subretinal space was achieved using a 29-gauge injection cannula, either blunted or with a 30° sharpened bevel, and partially ensheathed with moveable plastic tubing. Depending on the injection volume used, the retina detached, and the fluid was reabsorbed within 1–3 weeks, although for smaller volumes the retina reattached within a matter of days. The optimal injection volume used was between 100 and 150 μl, or two injections of 55 μl each. By ophthalmoscopy following the surgery, it …
Application Of A New Subretinal Injection Device In The Dog, András M. Komáromy, Signe E. Varner, Eugene De Juan, Gregory M. Acland, Gustavo D. Aguirre
Application Of A New Subretinal Injection Device In The Dog, András M. Komáromy, Signe E. Varner, Eugene De Juan, Gregory M. Acland, Gustavo D. Aguirre
Gustavo D. Aguirre, VMD, PhD
The use of a new subretinal injection device (RetinaJect™ Subretinal Cannula, SurModics, Inc., Eden Prairie, MN) to access the subretinal space in the canine model was evaluated. Subretinal injections were performed in 33 mongrel dogs between 2 and 52 months of age (median = 9 months). In 5 normal dogs the injection of 150 μl saline or India ink occurred by using a conventional subretinal injection device (CSID) with a 30-gauge anterior chamber irrigating cannula. The sclera had to be surgically exposed and penetrated before the subretinal injection with the CSID could occur. After removing the CSID, the conjunctiva over …