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Full-Text Articles in Ophthalmology
Decreased Expression Of Peroxiredoxins In Fuchs’ Endothelial Dystrophy, Ula V. Jurkunas, Ian Rawe, Maya Bitar, Cheng Zhu, Deshea L. Harris, Kathryn Colby, Nancy C. Joyce
Decreased Expression Of Peroxiredoxins In Fuchs’ Endothelial Dystrophy, Ula V. Jurkunas, Ian Rawe, Maya Bitar, Cheng Zhu, Deshea L. Harris, Kathryn Colby, Nancy C. Joyce
Ophthalmology
Purpose: To compare the relative expression of peroxiredoxin (Prx) proteins in normal human corneal endothelium with endothelium in corneas affected by Fuchs’ endothelial dystrophy (FED) and between normal human endothelium and epithelial/stromal tissue.
Methods: Human corneal endothelial cell-Descemet’s membrane (HCEC-DM) complexes from normal and FED corneal buttons were dissected from the epithelium/stroma. For proteomic analysis, HCEC-DM protein extracts were separated by using two-dimensional gel electrophoresis. Relative differences in protein spot density was analyzed. Proteins of interest, including Prx isoforms, were identified by MALDI-TOF (matrix-assisted desorption ionization-time of flight) mass spectrometry. Western blot analysis compared the relative expression of Prx isoforms …
Increased Clusterin Expression In Fuchs’ Endothelial Dystrophy, Ula V. Jurkunas, Maya Bitar, Ian Rawe, Deshea L. Harris, Kathryn Colby, Nancy C. Joyce
Increased Clusterin Expression In Fuchs’ Endothelial Dystrophy, Ula V. Jurkunas, Maya Bitar, Ian Rawe, Deshea L. Harris, Kathryn Colby, Nancy C. Joyce
Ophthalmology
Purpose: To compare the relative expression of peroxiredoxin (Prx) proteins in normal human corneal endothelium with endothelium in corneas affected by Fuchs’ endothelial dystrophy (FED) and between normal human endothelium and epithelial/stromal tissue.
Methods: Human corneal endothelial cell-Descemet’s membrane (HCEC-DM) complexes from normal and FED corneal buttons were dissected from the epithelium/stroma. For proteomic analysis, HCEC-DM protein extracts were separated by using two-dimensional gel electrophoresis. Relative differences in protein spot density was analyzed. Proteins of interest, including Prx isoforms, were identified by MALDI-TOF (matrix-assisted desorption ionization-time of flight) mass spectrometry. Western blot analysis compared the relative expression of Prx isoforms …