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Activation Of Hindbrain Neurons Is Mediated By Portal-Mesenteric Vein Glucosensors During Slow-Onset Hypoglycemia, Maryann Bohland, Aleksey V. Matveyenko, Maziyar Saberi, Arshad M. Khan, Alan G. Watts, Casey M. Donovan

Arshad M. Khan, Ph.D.

Hypoglycemic detection at the portal-mesenteric vein (PMV) appears mediated by spinal afferents and is critical for the counter-regulatory response (CRR) to slow-onset, but not rapid-onset, hypoglycemia. Since rapid-onset hypoglycemia induces Fos protein expression in discrete brain regions, we hypothesized that denervation of the PMV or lesioning spinal afferents would suppress Fos expression in the dorsal medulla during slow-onset hypoglycemia, revealing a central nervous system reliance on PMV glucosensors. Rats undergoing PMV deafferentation via capsaicin, celiac-superior mesenteric ganglionectomy (CSMG), or total subdiaphragmatic vagotomy (TSV) were exposed to hyperinsulinemic–hypoglycemic clamps where glycemia was lowered slowly over 60–75 min. In response to hypoglycemia, …