Medical Toxicology Commons^™

Anti-Cancer Effects Of Glypican-3 On Huh-7 Hepatocellular Carcinoma Cells, Jiyu Wen, Xiaojun Wen, Jinju Wang, Yang Shu, Zhidong Qiu, Zhongkao Liu, Ran Li, Guofang Zeng, Shiting Bao, Huilai Miao, Yanfang Chen, Mingyi Li

Pharmacology and Toxicology Faculty Publications

Aim: Previous studies have suggested Glypican-3 (GPC3) could be a valuable diagnostic marker for hepatocellular carcinoma. This study examined the effects of overexpression of GPC3 on Huh-7 hepatoma cells.

Methods: We constructed a recombinant plasmid vector pcDNA3.1 (+)-GPC3 for GPC3 overexpression studies in Huh-7 cells. RT-PCR and Western blotting were used to confirm GPC3 gene expression. Cell proliferation was evaluated by 5-ethynyl-2-deoxyuridine (EdU) incorporation assay. Cell cycle progression and apoptosis were determined by flow cytometry using propidium iodide (PI) and Annexin V-FITC/PI staining, respectively. Cell migration and invasion were examined by Boyden Transewll and Matrigel assays.

Results: GPC3 overexpression effectively …

The Preliminary Study Of Effects Of Tolfenamic Acid On Cell Proliferation, Cell Apoptosis, And Intracellular Collagen Deposition In Keloid Fibroblasts In Vitro, Dan Yi, Mackenzie S. Newman, Yanfang Chen, Richard Simman

Pharmacology and Toxicology Faculty Publications

Keloid scarring is a fibroproliferative disorder due to the accumulation of collagen type I. Tolfenamic acid (TA), a nonsteroidal anti-inflammatory drug, has been found to potentially affect the synthesis of collagen in rats. In this preliminary study, we aimed to test the effects of TA on cell proliferation, cell apoptosis, and the deposition of intracellular collagen in keloid fibroblasts. Normal fibroblasts (NFs) and keloid fibroblasts (KFs) were obtained from human dermis tissue. Within the dose range 10−3–10−6 M and exposure times 24 h, 48 h, and 72 h, we found that 0.55 × 10−3 M TA at 48 h exposure …