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Full-Text Articles in Medical Neurobiology
Distinct Properties Of Crac And Mic Channels In Rbl Cells, J. Ashot Kozak, Hubert H. Kerschbaum, Michael D. Cahalan
Distinct Properties Of Crac And Mic Channels In Rbl Cells, J. Ashot Kozak, Hubert H. Kerschbaum, Michael D. Cahalan
Neuroscience, Cell Biology & Physiology Faculty Publications
In rat basophilic leukemia (RBL) cells and Jurkat T cells, Ca2+ release–activated Ca2+ (CRAC) channels open in response to passive Ca2+ store depletion. Inwardly rectifying CRAC channels admit monovalent cations when external divalent ions are removed. Removal of internal Mg2+ exposes an outwardly rectifying current (Mg2+-inhibited cation [MIC]) that also admits monovalent cations when external divalent ions are removed. Here we demonstrate that CRAC and MIC currents are separable by ion selectivity and rectification properties: by kinetics of activation and susceptibility to run-down and by pharmacological sensitivity to external Mg2+, spermine, and …