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Full-Text Articles in Medical Neurobiology
The Role Of Ca2+/Calmodulin Dependent Protein Kinase Ii Alpha In Group 1 Metabotropic Glutamate Receptor Endocytosis And Signalling, Stephanie C. Kulhawy
The Role Of Ca2+/Calmodulin Dependent Protein Kinase Ii Alpha In Group 1 Metabotropic Glutamate Receptor Endocytosis And Signalling, Stephanie C. Kulhawy
Electronic Thesis and Dissertation Repository
Group 1 metabotropic glutamate receptors (mGluR1 and mGluR5) are G-protein coupled receptors (GPCRs) activated by glutamate. mGluR1/5 couples to Gαq/11 and releases Ca2+ from the endoplasmic reticulum. Ca2+/calmodulin-dependent protein kinase II alpha (CaMKIIα) can be activated by Gαq/11-mediated Ca2+ release through binding of Ca2+/calmodulin. Results from a proteomic screen identified CaMKII as a novel mGluR-interacting protein. Therefore, we hypothesized that CaMKIIα associates with group 1 mGluRs and this association alters mGluR1/5 signalling and internalization. Firstly, we demonstrated the novel association between CaMKIIα and mGluR1/5 by co-immunoprecipitation of transiently transfected proteins in …