Medical Genetics Commons^™

Dissection Of A Circadian Oscillation Into Discrete Domains, Martha W. Merrow, Norman Y. Garceau, Jay C. Dunlap

Dartmouth Scholarship

The circadian oscillator in Neurospora is a negative feedback loop involving as principal players the products of the frequency (frq) locus. frq encodes multiple forms of its protein product FRQ, which act to depress the amounts of frq transcript. In this scheme there are two discrete and separable steps to the circadian cycle, negative feedback itself (repression) in which FRQ acts to decrease the levels of its own transcript, and recovery from repression (derepression) in which frq transcript levels return to peak amounts. By introducing an exogenously regulatable frq transgene into a frq loss-of-function strain (frq^{9 …}