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Synergy Sted Multicolor 3d-Easysted Excel File, George Mcnamara

George McNamara

Excel 2007 file with calculations for multicolor 3D-EasySTED segmented waveplates (SWP) for fluorescence nanoscopy.

Additional explanation in my Introduction statement and in the Excel file. The Excel file uses an interpolation function available from XlXtrFun (http://www.xlxtrfun.com/XlXtrFun/XlXtrFun.htm). My thanks to Goetz Zinner, www.B-Halle.de, for communicating to me that a single 3D-EasySTED segmented waveplate can be used with multiple excitation and depletion wavelengths.

Please give Goetz Zinner full credit for recognizing that STED segmented waveplate can be manufactured to handle multiple excitation and depletion wavelengths. Different sets of wavelengths can be generated by choosing appropriate thickness quartz substrates for manufacturing the SWP. …