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The Differentiation Of Hepatocyte-Like Cells From Monkey Embryonic Stem Cells., Xiaocui Ma, Jian Wu, M Zern
The Differentiation Of Hepatocyte-Like Cells From Monkey Embryonic Stem Cells., Xiaocui Ma, Jian Wu, M Zern
jian duan wu
Embryonic stem cells (ESC) hold great potential for the treatment of liver diseases. Here, we report the differentiation of rhesus macaque ESC along a hepatocyte lineage. The undifferentiated monkey ESC line, ORMES-6, was cultured in an optimal culture condition in an effort to differentiate them into hepatocyte-like cells in vitro. The functional efficacy of the differentiated hepatic cells was evaluated using RT-PCR for the expression of hepatocyte specific genes, and Western blot analysis and immunocytochemistry for hepatic proteins such as alpha-fetoprotein (AFP), albumin and alpha1-antitrypsin (alpha1-AT). Functional assays were performed using the periodic acid schiff (PAS) reaction and ELISA. The …