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Full-Text Articles in Medical Biochemistry
Activation Of The Human Thymidine Kinase (Tk) Promoter By Simian Virus 40 Large T Antigen Requires Both The T Antigen Prb Family-Binding Domain And Tk Promoter Sequences Resembling E2f-Binding Sites., Michelle M. Anderson, Jun Chen, Charles N. Cole, Susan E. Conrad
Activation Of The Human Thymidine Kinase (Tk) Promoter By Simian Virus 40 Large T Antigen Requires Both The T Antigen Prb Family-Binding Domain And Tk Promoter Sequences Resembling E2f-Binding Sites., Michelle M. Anderson, Jun Chen, Charles N. Cole, Susan E. Conrad
Dartmouth Scholarship
Infection of quiescent cells with the DNA tumor virus simian virus 40 induces expression of the cellular thymidine kinase (TK) gene a minimum of 10- to 20-fold, and this induction depends upon the viral protein large T antigen (T-Ag). To define both human TK promoter elements and T-Ag functional domains required for transcriptional induction, we have established a system in which stable Rat-1 transfectants harboring TK promoter-luciferase hybrid genes are infected with recombinant adenoviruses expressing either wild-type or mutant forms of T-Ag and luciferase expression is measured as an indicator of promoter activity. The results show that (i) a 135-bp …
Distinct Cis-Acting Elements Mediate Clock, Light, And Developmental Regulation Of The Neurospora Crassa Eas (Ccg-2) Gene., Deborah Bell-Pedersen, Jay C. Dunlap, Jennifer J. Loros
Distinct Cis-Acting Elements Mediate Clock, Light, And Developmental Regulation Of The Neurospora Crassa Eas (Ccg-2) Gene., Deborah Bell-Pedersen, Jay C. Dunlap, Jennifer J. Loros
Dartmouth Scholarship
The Neurospora crassa eas (ccg-2) gene, which encodes a fungal hydrophobin, is transcriptionally regulated by the circadian clock. In addition, eas (ccg-2) is positively regulated by light and transcripts accumulate during asexual development. To sort out the basis of this complex regulation, deletion analyses of the eas (ccg-2) promoter were carried out to localize the cis-acting elements mediating clock, light, and developmental control. The primary sequence determinants of a positive activating clock element (ACE) were found to reside in a 45-bp region, just upstream from the TATA box. Using a novel unregulated promoter/reporter system developed for this study, we show …
Gtpase-Deficient G Alpha 16 And G Alpha Q Induce Pc12 Cell Differentiation And Persistent Activation Of Cjun Nh2-Terminal Kinases., Lynn E. Heasley, Brooke Storey, Gary R. Fanger, Laura Butterfield, J Zamarripa, D Blumberg, R A. Maue
Gtpase-Deficient G Alpha 16 And G Alpha Q Induce Pc12 Cell Differentiation And Persistent Activation Of Cjun Nh2-Terminal Kinases., Lynn E. Heasley, Brooke Storey, Gary R. Fanger, Laura Butterfield, J Zamarripa, D Blumberg, R A. Maue
Dartmouth Scholarship
Persistent stimulation of specific protein kinase pathways has been proposed as a key feature of receptor tyrosine kinases and intracellular oncoproteins that signal neuronal differentiation of rat pheochromocytoma (PC12) cells. Among the protein serine/threonine kinases identified to date, the p42/44 mitogen-activated protein (MAP) kinases have been highlighted for their potential role in signalling PC12 cell differentiation. We report here that retrovirus-mediated expression of GTPase-deficient, constitutively active forms of the heterotrimeric Gq family members, G alpha qQ209L and G alpha 16Q212L, in PC12 cells induces neuronal differentiation as indicated by neurite outgrowth and the increased expression of voltage-dependent sodium channels. Differentiation …