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Articles 1 - 5 of 5
Full-Text Articles in Medical Biochemistry
Efficient Transcriptional Activation Of Many Simple Modular Promoters By Simian Virus 40 Large T Antigen., Philip W. Rice, Charles N. Cole
Efficient Transcriptional Activation Of Many Simple Modular Promoters By Simian Virus 40 Large T Antigen., Philip W. Rice, Charles N. Cole
Dartmouth Scholarship
Simian virus 40 (SV40) large T antigen is a multifunctional protein which plays central roles during both lytic and transforming infections by SV40. It is a potent transcriptional activator and increases expression from the SV40 late promoter and from several cellular promoters. To understand better the transcriptional activation activity of large T antigen, we examined its ability to transactivate a set of simple modular promoters containing one of four upstream activation sequences coupled with one of three different TATA box sequences originally constructed and studied by Taylor and Kingston (Mol. Cell. Biol. 10:165-175, 1990). Large T antigen activated transcription from …
Cloning And Characterization Of Subunits Of The T-Cell Receptor And Murine Leukemia Virus Enhancer Core-Binding Factor., Shuwen Wang, Qing Wang, Barbara E. Crute, Irena N. Melnikova, Susanna R. Keller, Nancy A. Speck
Cloning And Characterization Of Subunits Of The T-Cell Receptor And Murine Leukemia Virus Enhancer Core-Binding Factor., Shuwen Wang, Qing Wang, Barbara E. Crute, Irena N. Melnikova, Susanna R. Keller, Nancy A. Speck
Dartmouth Scholarship
Moloney murine leukemia virus causes thymic leukemias when injected into newborn mice. A major determinant of the thymic disease specificity of Moloney virus genetically maps to the conserved viral core motif in the Moloney virus enhancer. Point mutations introduced into the core site significantly shifted the disease specificity of the Moloney virus from thymic leukemia to erythroid leukemia (N.A. Speck, B. Renjifo, E. Golemis, T.N. Fredrickson, J.W. Hartley, and N. Hopkins, Genes Dev. 4:233-242, 1990). We previously reported the purification of core-binding factors (CBF) from calf thymus nuclei (S. Wang and N.A. Speck, Mol. Cell. Biol. 12:89-102, 1992). CBF binds …
Isolation Of Interleukin 2-Induced Immediate-Early Genes., Carol Beadling, Kirk W. Johnson, Kendall A. Smith
Isolation Of Interleukin 2-Induced Immediate-Early Genes., Carol Beadling, Kirk W. Johnson, Kendall A. Smith
Dartmouth Scholarship
Clonal expansion of antigen-reactive T lymphocytes is driven by the lymphokine interleukin 2 (IL-2). To further elucidate the mechanisms of IL-2 action, we have utilized a differential hybridization procedure to clone IL-2-induced immediate-early genes from an IL-2-stimulated human T-cell cDNA library. To increase the frequency of IL-2-induced transcripts represented in the library, the protein synthesis inhibitor cycloheximide was included during the 2-hr IL-2 stimulation to superinduce gene expression, and the uridine analogue 4-thiouridine was utilized to enable selective purification of newly synthesized transcripts. From the enriched library, we have isolated eight IL-2-induced genes, six of which represent previously unrecognized human …
Two Factors That Bind To Highly Conserved Sequences In Mammalian Type C Retroviral Enhancers., Nancy R. Manley, Mary M. O'Connell, Wanwen Sun, Nancy A. Speck, Nancy Hopkins
Two Factors That Bind To Highly Conserved Sequences In Mammalian Type C Retroviral Enhancers., Nancy R. Manley, Mary M. O'Connell, Wanwen Sun, Nancy A. Speck, Nancy Hopkins
Dartmouth Scholarship
The transcriptional enhancers of the Moloney and Friend murine leukemia viruses (MLV) are important determinants of viral pathogenicity. We used electrophoretic mobility shift and methylation interference assays to study nuclear factors which bind to a region of these enhancers whose sequence is identical between Moloney and Friend viruses and particularly highly conserved among 35 mammalian type C retroviruses whose enhancer sequences have been aligned (E. Golemis, N. A. Speck, and N. Hopkins, J. Virol. 64:534-542, 1990). Previous studies identified sites for the leukemia virus factor b (LVb) and core proteins in this region (N. A. Speck and D. Baltimore, Mol. …
Characterization Of A Protein That Binds Multiple Sequences In Mammalian Type C Retrovirus Enhancers., Wanwen Sun, Mary M. O'Connell, Nancy A. Speck
Characterization Of A Protein That Binds Multiple Sequences In Mammalian Type C Retrovirus Enhancers., Wanwen Sun, Mary M. O'Connell, Nancy A. Speck
Dartmouth Scholarship
Mammalian type C retrovirus enhancer factor 1 (MCREF-1) is a nuclear protein that binds several directly repeated sequences (CNGGN6CNGG) in the Moloney and Friend murine leukemia virus (MLV) enhancers (N. R. Manley, M. O'Connell, W. Sun, N. A. Speck, and N. Hopkins, J. Virol. 67:1967-1975, 1993). In this paper, we describe the partial purification of MCREF-1 from calf thymus nuclei and further characterize the binding properties of MCREF-1. MCREF-1 binds four sites in the Moloney MLV enhancer and three sites in the Friend MLV enhancer. Ethylation interference analysis suggests that the MCREF-1 binding site spans two adjacent minor grooves of …