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Articles 1 - 2 of 2
Full-Text Articles in Medical Biochemistry
Molecular Cloning And Sequence Analysis Of The Plasmodium Falciparum Dihydrofolate Reductase-Thymidylate Synthase Gene., David J. Bzik, Wu-Bo Li, Toshihiro Horii, Joseph Inselburg
Molecular Cloning And Sequence Analysis Of The Plasmodium Falciparum Dihydrofolate Reductase-Thymidylate Synthase Gene., David J. Bzik, Wu-Bo Li, Toshihiro Horii, Joseph Inselburg
Dartmouth Scholarship
Genomic DNA clones that coded for the bifunctional dihydrofolate reductase (DHFR) and thymidylate synthase (TS) (DHFR-TS) activities from a pyrimethamine-sensitive strain of Plasmodium falciparum were isolated and sequenced. The deduced DHFR-TS protein contained 608 amino acids (71,682 Da). The coding region for DHFR-TS contained no intervening sequences and had a high A + T content (75%). The DHFR domain, in the amino-terminal portion of the protein, was joined by a 94-amino acid junction sequence to the TS domain in the carboxyl-terminal portion of the protein. The TS domain was more conserved than the DHFR domain and both P. falciparum domains …
Identification Of A Complex Associated With Processing And Polyadenylation In Vitro Of Herpes Simplex Virus Type 1 Thymidine Kinase Precursor Rna., Fang Zhang, Charles N. Cole
Identification Of A Complex Associated With Processing And Polyadenylation In Vitro Of Herpes Simplex Virus Type 1 Thymidine Kinase Precursor Rna., Fang Zhang, Charles N. Cole
Dartmouth Scholarship
Cleavage and polyadenylation of substrate RNAs containing the herpes simplex virus type 1 (HSV-1) thymidine kinase (tk) gene polyadenylation signal region were examined in HeLa cell nuclear extract. 3'-End RNA processing was accurate and efficient and required ATP and Mg2+. Cleavage, but not polyadenylation, occurred in the presence of EDTA or when ATP was replaced with 3' dATP (cordycepin) or AMP(CH2)PP, a nonhydrolyzable analog of ATP. Processing in vitro and in vivo showed the same signal element requirements: a series of substrates containing linker scanning, internal deletion, and small insertion mutations was processed with the same relative efficiencies and at …