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Isolation Of Plasma Membrane From Human Neutrophils And Determination Of Cytochrome B And Quinone Content, Edward P. Sloan, Dana R. Crawford, Donald L. Schneider
Isolation Of Plasma Membrane From Human Neutrophils And Determination Of Cytochrome B And Quinone Content, Edward P. Sloan, Dana R. Crawford, Donald L. Schneider
Dartmouth Scholarship
Analyses of plasma membrane and other subcellular fractions indicate that the primary location of cytochrome b in human neutrophils is not the plasma membrane. The procedure developed for the purification of plasma membrane from fresh human neutrophils yielded a 14-fold enrichment in the marker enzyme 5'-nucleotidase and a 10-fold enrichment in ouabain-sensitive ATPase. On sucrose density gradients, the peak density of 5'-nucleotidase activity was 1.12 g/ml, and was shifted after digitonin addition to 1.15 g/ml. Protein in the plasma membrane equalled approximately 8 percent of the whole cell protein. A b-type cytochrome was found to be present in the plasma …