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The Envelope Stress Response In Sedimentation-Resistant Escherichia Coli, Neel K. Shah
The Envelope Stress Response In Sedimentation-Resistant Escherichia Coli, Neel K. Shah
Honors Undergraduate Theses
Previous research discovered the existence of sedimentation-resistant mutants of E. coli. Genomic studies revealed that these mutants resisted sedimentation due to independent modifications to genes that influenced the Rcs signal transduction pathway, causing increased secretion of an exopolysaccharide capsule comprised primarily of colanic acid. The Rcs system is responsible for detecting envelope stressors; consequently, ampicillin and osmotic stress were used to perturb the cellular envelope and study the response of the mutants compared to wild-type cells. It was found that the overproduction of colanic acid in the mutants confers some resistance to envelope stress; however, the mutants still behaved …