Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Dental Materials
Toxicity Of Root Canal Filling Materials On Hela Cells And Fibroblasts, Eldon P. Carman
Toxicity Of Root Canal Filling Materials On Hela Cells And Fibroblasts, Eldon P. Carman
Loma Linda University Electronic Theses, Dissertations & Projects
A study was undertaken to determine the concentration at which a sealer reaches a point of nontoxicity. An in vitro model utilizing HeLa cells and human fibroblasts was used to observe the effect of various concentrations of eight freshly mixed and eight set root canal sealers. Evaluation of toxicity was determined by cell survival which was measured by incorporation of 3H-thymidine into the nucleic acid components of each cell type used. Each dilution tested was allowed direct contact with the cells for 24 hours before toxicity evaluation. The results indicate that all sealers at concentrations presently used are cytotoxic …