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Viability Of Human Periodontal Ligament Fibroblasts After Time In Tooth Storage Media, Scott Macdonald

Master's Theses (2009 -)

Periodontal ligament cell viability at the time of tooth replantation is of utmost importance for proper repair of the damaged tissues (1). Multiple different options are currently available for storage of an avulsed tooth. The goal of this study was to investigate new storage media and evaluate their ability to maintain cell viability over time. Materials and Methods: Cultured human periodontal ligament fibroblasts (hPDLF) were seeded on 96-well plates. These cells were exposed to different storage media including: Hank’s Balanced Salt Solution (HBSS), EMT Toothsaver (EMT), Save-A-Tooth (SAT), trehalose (T300), cell culture media, water, and different formulations of HBSS combined …