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Full-Text Articles in Amino Acids, Peptides, and Proteins
Recombinant Expression And Purification Of Amyloid-Β In E. Coli, Wisam M. Beauti
Recombinant Expression And Purification Of Amyloid-Β In E. Coli, Wisam M. Beauti
Honors Theses
Alzheimer’s disease (AD) is a common neurodegenerative disorder that affects people older than 65 years 1,2,5. It is characterized by the presence of extracellular plaque deposits that are seen specifically in the brains of AD patients 4,5. These plaques are mainly comprised of amyloid-β (Aβ) peptide aggregates. Aβ plaque production and deposition is believed to drive AD pathogenesis. Studying these proteins is crucial to understanding aspects of AD in order to develop possible therapeutic treatments. Recombinant expression of Aβ can also provide a handle to introduce mutations in the protein to further study their structure-function relationships. However, synthetic Aβ monomer …
Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese
Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese
Honors Theses
In the fields of protein science and medicine, understanding the kinetics of protein aggregation are significant in the research and treatment of certain amyloid diseases such as Alzheimer’s. Previous studies have suggested that arginine can increase the solubility of certain proteins, suppress protein aggregation, and assist in the refolding of aggregated proteins; however, the molecular mechanisms by which arginine can influence protein aggregation are still unclear. Bovine insulin was employed as a model system for further understanding the effects of arginine on protein aggregation. Using Dynamic Light Scattering (DLS), we studied the concentration-dependent and temperature-dependent suppression of aggregation in insulin …