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Development Of Rp-Hplc Method For Simultaneous Determination Of Nebivolol Hydrochloride, Valsartan And Hydrochlorothiazide In Co-Administrated Antihypertensive Agents And Human Plasma, Shereen Mowaka, M. Rizk, Maha M. Abou El‐Alamin, Mariam Mohamed
Development Of Rp-Hplc Method For Simultaneous Determination Of Nebivolol Hydrochloride, Valsartan And Hydrochlorothiazide In Co-Administrated Antihypertensive Agents And Human Plasma, Shereen Mowaka, M. Rizk, Maha M. Abou El‐Alamin, Mariam Mohamed
Pharmacy
Reversed phase high performance liquid chromatography combined with photodiode array (HPLC-PDA) detector method was developed for simultaneous determination of two binary antihypertensive combinations; either Nebivolol (NEB) with Hydrochlorothiazide (HCZ) or with Valsartan (VAL) in pharmaceutical formulation and spiked human plasma. The method was successfully applied to separate the three drugs on one chromatogram under the same chromatographic conditions that used for two binary combinations. The separation was established on Xterra C18 column using gradient mobile phase which composed of acidified water of pH = 3.0 (adjusted with 0.1% orthophosphoric acid) and acetonitrile with flow rate of 1.0 mL/min. The drugs …
The Potential Off-Target Neuroprotective Effect Of Sister Gliflozins Suggests Their Repurposing Despite Not Crossing The Blood–Brain Barrier: From Bioanalytical Assay In Rats Into Theory Genesis, Shereen Mowaka, Moataz S. Hendy, Ehab F. Elkady, Asmaa El-Zaher, Bassam M. Ayoub
The Potential Off-Target Neuroprotective Effect Of Sister Gliflozins Suggests Their Repurposing Despite Not Crossing The Blood–Brain Barrier: From Bioanalytical Assay In Rats Into Theory Genesis, Shereen Mowaka, Moataz S. Hendy, Ehab F. Elkady, Asmaa El-Zaher, Bassam M. Ayoub
Pharmacy
Gliflozins are successfully marketed antidiabetic agents with a reported neuroprotective effect, and this study tests their blood–brain barrier crossing ability. Henceforward, a computational hypothesis interpreting their effects was reasonable after failure to cross into the brain. A chromatographic bioassay for canagliflozin, dapagliflozin, and empagliflozin was developed, validated, and applied to the rat's and rat's plasma and brain. HPLC method robustness was tested over two levels using Design of Experiment on MINITAB. It is the first method for gliflozins’ detection in rats’ brain tissue. The method was applied on 18 rats and six for each drug. Concentrations in plasma were determined …