Medicine and Health Sciences Commons^™

Dissociation Of Pulse Wave Velocity And Aortic Wall Stiffness In Diabetic Db/Db Mice: The Influence Of Blood Pressure., Patricia E Mccallinhart, Yong Ung Lee, Avione Lee, Mircea Anghelescu, Jeffrey R Tonniges, Ed Calomeni, Gunjan Agarwal, Joy Lincoln, Aaron J Trask

PCOM Scholarly Papers

Introduction: Vascular stiffness is a predictor of cardiovascular disease and pulse wave velocity (PWV) is the current standard for measuring in vivo vascular stiffness. Mean arterial pressure is the largest confounding variable to PWV; therefore, in this study we aimed to test the hypothesis that increased aortic PWV in type 2 diabetic mice is driven by increased blood pressure rather than vascular biomechanics. Methods and Results: Using a combination of in vivo PWV and ex vivo pressure myography, our data demonstrate no difference in ex vivo passive mechanics, including outer diameter, inner diameter, compliance (Db/db: 0.0094 ± 0.0018 mm2/mmHg vs. …

The Effects Of Metformin, Aminoguanidine, And Pyridoxamine On Methylglyoxal Induced Cardiac Myocyte Injury, Amanda Harvey, Rose Martorana, Julia Colangeli, Hafsa Ahmed, Dean Delgado, Alisa Kim, Robinderpaul Sandhu, Robert J. Barsotti, Lindon H. Young, Qian Chen

Research Day

Advanced glycation end (AGE) products have been under investigation for their role in cardiovascular disease in complications. Methylglyoxal, a byproduct of glucose metabolism, is elevated in the blood of diabetic patients and has shown to be an important intermediate in the production of AGEs. Due to the highly reactive structure of methylglyoxal, it can produce high levels of reactive oxygen species (ROS) within the cell via mitochondrial modification which results in a loss of cell membrane potential and possible cell death. The role of methylglyoxal in cardiac cells is not well known; in this study methylglyoxal induced cell injury and …

Synergistic Effects Of Methylglyoxal And Hyperglycemia On Ros Generation And The Viability Of Cultured H9c2 Myoblast Cells, Robinderpal Sandhu, Dylan Lefkovitz, Al Ibe, Alisa Kim, Peter Wieczorek, Dean Delgado, Robert J. Barsotti, Lindon H. Young, Qian Chen

Research Day

Heart damage in diabetics may be closely related to the possible synergistic cellular damage from hyperglycemia and increased methylglyoxal levels. This study investigated the effects of glucose and/or methylglyoxal and/or metformin on H9c2 reactive oxygen species (ROS) generation measured by a dichlorofluorescein diacetate (DCFDA) assay and cell viability measured by a cell counting kit-8 assay after various treatments for 24 hours.

Glucose treatment (5 mM-40 mM) displayed similar cell viability (n=4) and ROS generation (n=7) when compared to control cells. By contrast, methylglyoxal (5 µM-1400 µM) decreased cell viability at higher concentration (1000 µM (51 ± 8%); 1200 µM (41 …

Can A Vegetarian Diet Improve Quality Of Life In Type 2 Diabetics, Compared To Other Diabetic Diets?, Carly A. Kindbom

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: The objective of this selective EBM review is to determine if a vegetarian diet is more effective in improving quality of life in Type 2 Diabetics compared to other diabetic diets.

STUDY DESIGN: Review of two randomized control trials published in 2011 and 2013, and one prospective clinical intervention study published in 2010.

DATA SOURCES: Data sources were articles published in peer review journals found using PubMed and Cochrane Databases.

OUTCOMES MEASURED: Quality of life was measured by amount of weight loss from baseline and Obesity and Weight-Loss Quality of Life (OWLQOL) questionnaires.

RESULTS: Ferdowsian et al (2010) reported …

Do Inhaled Corticosteroids Cause An Increased Risk For Developing Or Worsening A Patient’S Diabetes Mellitus?, Jarrod R. Luttjohann

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE The objective of this selective EBM review is to determine whether or not inhaled corticosteroids (ICS) cause an increased risk for developing or worsening a patient’s diabetes mellitus (DM).

STUDY DESIGN Review of three English language primary studies published in 1993, 2009 and 2010.

DATA SOURCES A nested case-control analysis, a randomized control trial and a prospective randomized, doubleblind, double-dummy, placebo-controlled, crossover investigation comparing the onset and/or progression of diabetes mellitus in patients using ICS were found using Ovid MEDLINE, PubMed, and Cochrane databases.

OUTCOMES MEASURED Incidence or progression of diabetes was measured using a combination of oral glucose …

Is Acupuncture An Effective Treatment For Diabetic Peripheral Neuropathy?, Sherri A. Kowalczuk

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: The objective of this selective EBM review is to determine whether or not acupuncture is an effective treatment for diabetic peripheral neuropathy

STUDY DESIGN: Review of three English language primary studies, one published in 2007 and two of which were published in 2010.

DATA SOURCES: Randomized controlled trials investigating the use of acupuncture for the treatment of diabetic peripheral neuropathy (DPN). Sources were found using PubMed and Cochrane databases.

OUTCOMES MEASURED: The outcomes measured include the severity of pain from DPN, blood glucose control, and effects on quality of life such as sleep, mood states, and gain or loss …