Medicine and Health Sciences Commons^™

Self-Reported Racism, Transphobia, Their Intersection And Impact On Past-Year Hiv Related Sexual Risk Behaviour, Roxanne M. Longman Marcellin

Electronic Thesis and Dissertation Repository

Background: Studies examining HIV prevalence and risk behaviors within trans subgroups have identified them as high risk. Yet few studies have addressed how discrimination impacts this prevalence. Minority stress theory suggests that there is a relationship between minority stress and HIV-related risk behaviour. We hypothesize that multiple minority statuses may result in discriminatory experiences, specifically self-reported transphobia and racism in synchrony with other attributes, that interact to alter past-year HIV-related risk behaviour.

Methods: Data came from the Trans PULSE project, a mixed-methods, community-based research study that used respondent-driven sampling to access 433 trans Ontarians, between May 2009 and …

Peptide-Mediated Targeting Of Angiogenesis For Molecular Imaging And Treatment Of Cancer, Choi-Fong Cho

Electronic Thesis and Dissertation Repository

The development of screening approaches to identify novel affinity ligands has paved the way for a new generation of molecular targeted nanomedicines. To identify novel targeting ligands, several studies have demonstrated the advantages in screening one-bead-one-compound (OBOC) libraries. Conventional methods typically bias the display of the target protein to ligands during the screening process. We have developed an unbiased multiplex ‘beads on a bead’ strategy to isolate, characterize, and validate high affinity ligands from OBOC libraries. In addition, due to the advantages associated with screening OBOC libraries directly against living cells, we sought to combine cell-based screen methods with automated …

The Role Of Aldhhicd44+ Cells In Breast Cancer Metastasis And Therapy Resistance, Alysha K. Croker

Electronic Thesis and Dissertation Repository

Breast cancer is a leading cause of death in women, due primarily to ineffective treatment of metastatic disease. Despite being a lethal process, metastasis is surprisingly inefficient, with less than 0.5% of cancer cells able to successfully navigate the metastatic cascade, indicating that only a small proportion of cancer cells possess the necessary characteristics to establish metastases. Cancer stem cells (CSCs) have recently been identified in leukemia and solid tumors; however, the role of CSCs in breast cancer metastasis and therapy resistance remains poorly understood. Sub-populations of cells demonstrating stem-cell like characteristics (high expression of CSC markers and/or high ALDH …

Properties And Applications Of Sensory Outcome Measures In Carpal Tunnel Syndrome, Derek Km Cheung

Electronic Thesis and Dissertation Repository

This thesis aimed to determine the psychometric properties and applications of sensory threshold tools and outcome measures in patients with Carpal Tunnel Syndrome (CTS). The first chapter is a psychometric study that defines clinically important difference (CID), construct validity and responsiveness of touch and vibration threshold tools and in the Symptoms Severity Scale (SSS). The study found the CID for the PSSD and for the SSS was 0.15g/mm² and 0.50 respectively. The study also found that the Vibrometer was more representative of hand function and responsive compared PSSD. The second objective of this thesis was to determine the feasibility …

The Role Of Ca2+/Calmodulin Dependent Protein Kinase Ii Alpha In Group 1 Metabotropic Glutamate Receptor Endocytosis And Signalling, Stephanie C. Kulhawy

Electronic Thesis and Dissertation Repository

Group 1 metabotropic glutamate receptors (mGluR1 and mGluR5) are G-protein coupled receptors (GPCRs) activated by glutamate. mGluR1/5 couples to Gα_q/11 and releases Ca²⁺ from the endoplasmic reticulum. Ca²⁺/calmodulin-dependent protein kinase II alpha (CaMKIIα) can be activated by Gα_q/11-mediated Ca²⁺ release through binding of Ca²⁺/calmodulin. Results from a proteomic screen identified CaMKII as a novel mGluR-interacting protein. Therefore, we hypothesized that CaMKIIα associates with group 1 mGluRs and this association alters mGluR1/5 signalling and internalization. Firstly, we demonstrated the novel association between CaMKIIα and mGluR1/5 by co-immunoprecipitation of transiently transfected proteins in …

Parents' Perceptions Of Activity Restrictions In Children With Epilepsy: First Two Years Post-Diagnosis, Nathan C. King

Electronic Thesis and Dissertation Repository

Children with epilepsy are less physically and socially active than their peers. The objective is to explore whether parents represent a barrier to children’s activity, by examining associations between child and family factors and parents’ perceptions of epilepsy-related activity restrictions. Data were from the Health Related Quality of Life in Children with Epilepsy Study, a longitudinal study of children 4-12 years old with new-onset epilepsy. Parents reported on activity restrictions and family factors and neurologists reported on epilepsy-related characteristics at baseline, 6, 12, and 24 months. Linear mixed models were used to model relationships among child and family factors and …

Screening For Adult Adhd In Ontario: A Cross-Sectional Study Examining Sex Differences, Mental Health Correlates And Substance Use, Deanne Daigle

Electronic Thesis and Dissertation Repository

The vast majority of studies on attention-deficit/hyperactivity disorder (ADHD) are based on samples with inherent age, sex, and referral biases. Therefore, the current study used population-based data to 1) estimate the prevalence of adult ADHD (as well as previous diagnosis and medication use using an ADHD screener) and co-occurring psychiatric distress and substance use in Ontario 2) examine the sex differences in ADHD screening status and co-occurring psychiatric distress and substance use and 3) model ADHD screening status as a risk factor for psychiatric distress using the 2011 cycle of the Centre for Addiction and Mental Health Monitor. A positive …

Immunohistochemical Studies Of Expression And Correlation Of Osteopontin, Cd44, And Integrin Αvβ3 In Selected Benign And Malignant Salivary Gland Tumours, Tommy Fok

Electronic Thesis and Dissertation Repository

Osteopontin (OPN) is a phosphorylated acidic glycoprotein that has been shown to play a vital role in tumourigenesis and metastases. The objective of this study was to determine the expression of OPN and its receptors (CD44 and integrin α_vβ₃) in several salivary gland cancers, and assess its clinical correlations.

Immunohistochemical techniques were used to semi-quantify the level of OPN expression and its receptors in normal salivary glands and different types of salivary gland tumour.

Our results show that in tumours of salivary gland origin, higher levels of OPN were detected in tumours which were malignant compared …

Antenatal Care Determines Immunization And Care For Fever And Diarrhea In Ghanaian Children, Natalie K. Mcglynn

Electronic Thesis and Dissertation Repository

Enhancing maternal and child health comprise key Millennium Development Goals (MDGs), driving an analysis of whether efforts to improve maternal health through quality antenatal care (ANC) is associated with the recommended health-related care of children in Sub-Saharan Africa, where under-five mortality is the highest.

This study examines ANC as a potential determinant of immunization and appropriate care for fever and diarrhea in Ghanaian children. Data were pooled from the 1998, 2003, and 2008 Ghana Demographic Health Surveys, and Andersen’s Behavioural Model provided the conceptual framework.

Multivariate logistic regression indicated that quality ANC increases the odds of immunization and appropriate care …

Roles Of Pgc-1Α/Ppars Pathway In Regulating Insulin Sensitivity In Mouse Skeletal Muscle Cells Under Prolonged Hypoxia, Yunyan Zhang

Electronic Thesis and Dissertation Repository

Using the C₂C₁₂ mouse myoblast cell line, I investigated how prolonged hypoxia affected components of the insulin signalling and FAO/PGC-1α/PPARs pathways, as they might impact insulin sensitivity in skeletal muscle cells.

Hypoxia resulted in lower p-Akt (Thr 308) and higher total cellular GLUT4 protein levels after 7 days of differentiation. This coincided with higher triglyceride content and alterations of the FAO/PGC-1α/PPARs components, both of which could contribute to the changes observed in the components of the insulin signalling pathway. Specifically, cells differentiating in 1% O₂ had lower SIRT1, PPAR- α, FATP4 and MCAD mRNA; accompanied by …

The Role Of Non-Canonical Wnt/Planar Cell Polarity Signalling In Breast Cancer Progression, Connor D. Macmillan

Electronic Thesis and Dissertation Repository

The role of planar cell polarity (PCP) signalling in breast cancer is unclear, as evidence shows that activation of this pathway with WNT5A can either promote or inhibit progression. Using the 21T cells, which represent distinct stages of breast cancer progression when grown in a mouse xenograft model (21PT, atypical ductal hyperplasia; 21NT ductal carcinoma in situ; 21MT-1, invasive mammary carcinoma), I hypothesized that WNT5A will promote progression only when VANGL1, another component of PCP signalling, is elevated.

Each 21T cell line showed distinct stage-specific morphologies when grown in a Matrigel “on-top” assay. WNT5A overexpression promoted progression to an invasive …

Investigating Meaning In Life, Perceived Social Support, And Suicide Ideation Among Community-Residing Older Adults, Joyce L. Cheng

Electronic Thesis and Dissertation Repository

Older adults have high rates of suicide, necessitating enhanced models of suicide risk in later life. Previous cross-sectional findings suggest that suicide ideation is positively associated with psychological risk factors and negatively associated with resilience factors, including recognition of meaning in life (MIL) and perceptions of social support (PSS). We designed the current longitudinal study to replicate and extend these findings and to test a multidimensional model of suicide ideation (SI), which incorporates consideration of risk and resilience factors, among a sample of 173 community-residing older adults. Using Structural Equation Modeling, both MIL and PSS, which were measured at the …

Prolonged Treadmill Walking In Healthy Participants With And Without The Use Of Foot Orthoses To Support The Medial Longitudinal Arch, Daisy K. Ng

Electronic Thesis and Dissertation Repository

In the research setting, instrumented treadmills are often used to study prolonged periods of walking. This thesis examines the effects of in-shoe foot orthoses on walking gait during prolonged periods of treadmill walking. The two types of foot orthoses investigated were: 1) a pedorthist hand-made orthotic with medial longitudinal arch (MLA) support and 2) a proprioceptive feedback-type orthotic designed to stimulate the intrinsic foot muscles of the MLA. The three kinematic variables observed over 60 minutes of intermittent treadmill walking were toe-out angle in the transverse plane, pelvic tilt angle in the sagittal plane, and trunk lean angle in the …

Investigating Personality Vulnerability To Suicide Ideation In Community-Residing Older Adults, James Andrew Mcclure

Electronic Thesis and Dissertation Repository

This study assessed associations between suicide ideation and personality characteristics in a sample of community-residing adults 65 years of age and older. Measures of personality, suicide ideation, and depression were administered at two time points 6-12 months apart. Multiple linear regression analyses were employed to investigate cross-sectional and longitudinal associations between personality characteristics and suicide ideation. A total of 106 older adults (mean age=74.2 years, SD=5.8, including 78 women) completed both phases of the study. The majority of participants included in this study were relatively emotionally and psychologically healthy. Within this sample of older adults, more severe suicide ideations was …

Gender Does Not Influence The Relationship Between Posterior Cruciate Ligament Design And Patient-Reported Outcomes In Patients Receiving Primary Unilateral Total Knee Arthroplasty, Ryan Milan

Electronic Thesis and Dissertation Repository

The effect of the interaction between gender and posterior cruciate ligament (PCL) prosthesis design on patient-reported outcomes is an understudied area of research. We evaluated 1613 patients, from the Ontario Joint Replacement Registry (2001-2006), who underwent primary total knee replacement. This study investigated the impact of the gender-PCL design interaction on Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) change scores and patient satisfaction, by performing linear regression analysis, using full-adjusted models that also included the gender-PCL prosthesis design interaction variable. PCL prosthesis design did not affect WOMAC change scores or satisfaction (p>0.05). Moreover, Gender did not influence either …

Regulation Of Nk Cell-Mediated Tubular Epithelial Cell Death And Kidney Ischemia-Reperfusion Injury By The Nkr-P1b Receptor And Clr-B, James Yip

Electronic Thesis and Dissertation Repository

Renal ischemia-reperfusion injury (IRI) occurs following reduced renal blood flow and is a major cause of acute injury in both native and transplanted kidneys. We have previously demonstrated that NK cells can mediate tubular cell death and kidney IRI. Natural killer receptor-protein 1B (NKR-P1B) has been shown to interact with C-type lectin-related protein B (Clr-b), resulting in the suppression of NK cell-mediated cytotoxicity.

Clr-b mRNA and protein expression in the kidney were up-regulated after renal IRI. Similar upregulation of Clr-b expression was seen in cytokine-challenged primary-cultured tubular epithelial cells (TEC). Furthermore, NK cytotoxicity assays demonstrated enhanced necrotic death in TEC …

The Impact Of The Neuropeptide Y System On The Progression Of Breast Cancer, Philip J. Medeiros

Electronic Thesis and Dissertation Repository

Chronic stress is associated with elevated levels of sympathetic neurotransmitter release and immunosuppression. A growing body of evidence suggests that stress-related factors may contribute to the initiation, development and progression of breast cancer. Neuropeptide Y (NPY) has recently been determined as such a factor, however few investigations have addressed the functional effects of NPY on breast cells and tumors. Thus, the overall objective of this thesis was to elucidate the mechanisms by which NPY may contribute to the disease. Using the 4T1 murine breast cancer model, we began by first characterizing NPY receptor expression in cancer cells and tumors. Y1R, …

Involvement Of Interleukin-33/St2 In Myocardial Dysfunction In Murine Model Of Sepsis, Yoonmi Choe

Electronic Thesis and Dissertation Repository

The disruption of myocardial extracellular matrix (ECM) has been implicated in myocardial dysfunction during sepsis. However, the underlying mechanism(s) are not clear. Interleukin-33 (IL-33) is a cytokine which regulates collagen synthesis in various cardiac pathologies. The purpose of the present study is to test whether IL-33 contributes to sepsis-induced myocardial dysfunction through regulation of matrix metalloproteinase-9 (MMP-9). The in vivo, feces-induced peritonitis (FIP) in mice and in vitro lipopolysaccharide (LPS) treatments to isolated cardiomyocytes were used. In FIP mice, myocardial IL-33 and MMP-9 expression were increased and myocardial contractility was decreased. Myocardial function in FIP mice was improved when treated …

Clinical Instructors' Perceptions Of Structural And Psychological Empowerment In Academic Nursing Environments, Sandra E. Wiens

Electronic Thesis and Dissertation Repository

ABSTRACT

The study purpose was to explore clinical instructors’ perceptions of empowerment in academic nursing environments. Clinical instructors (CIs), often part-time faculty, facilitate learning in professional practice environments. However, they also need to function within the academic environment to learn about the curriculum and how students are to be evaluated.

The qualitative description method was used to obtain a broad understanding of CIs’ empowerment experiences and to interpret their perceptions with the framework of Kanter’s (1977, 1993) structural empowerment and Spreitzer’s (1995) psychological empowerment theories. Eight CIs from two nursing programs were interviewed.

All empowerment components were important in CIs’ …

Identifying The Influence Of Parents' And Children's Perceptions Of Their Built And Social Environments On Children's Mode Of Travel To And From School, Emily S. Hill

Electronic Thesis and Dissertation Repository

This study examined the influence of parents’ and children’s perceptions of their built and social environments on children’s use of active transportation (AT) between home and school. A mixed-methods approach was used including an environmental/behavioural survey completed by students (grades 5 through 8) and parents from 32 schools throughout London, Ontario, (n=1,623); Geographic Information Systems (GIS) for generating built environment variables to be controlled for; Principal Component factor analysis; and step-wise logistic regression models that divided analysis between the journey to and from school to determine the most influential factors in either direction. Children’s personal attitudes were the …

Family Physician Access Among Trans People In Ontario: A Cross-Sectional Analysis Of Social Determinants Of Health And Inequality Issues., Xuchen Zong

Electronic Thesis and Dissertation Repository

For trans Ontarians with access to publicly insured health care, this study aimed to determine predictors of not having a family physician, as well as to identify factors that influence a trans patient’s comfort discussing trans status or trans-related health needs with their physician. Previously collected demographic and family physician access related data (n=433) were used. Multiple logistic predictive model showed that age, marital status, education, employment, income-to-needs ratio, and social support independently predicted not having a family physician. Marital status, negative trans-specific experience with family physicians, and perception of family physician's knowledge about trans health needs were identified as …

Optimization Of Chiral Separation Of Nadolol By Simulated Moving Bed Technology, Nesma Nehad Hashem

Electronic Thesis and Dissertation Repository

Simulated Moving Bed (SMB) technology has gained increasing attention as one of the most powerful techniques for chromatographic separations due to its cost-effectiveness and efficiency. Application of SMB technology is especially important in the pharmaceutical industry for production of enantiopure drugs, as required under strict FDA regulations, to avoid possible adverse effects of racemic drugs. In this study, the performance of the SMB process in separation of racemic nadolol on a perphenyl carbamoylated beta cyclodextrin (β-CD) stationary phase was investigated. The equilibrium dispersive model coupled with bi-Langmuir adsorption isotherm and lumped kinetic approximation, constitute the mathematical model used to simulate …

Experimental And Computational Analysis Of The Structure And Dynamics Of Intrinsically Disordered Proteins, Elio Anthony Cino

Electronic Thesis and Dissertation Repository

Intrinsically disordered proteins (IDPs) are abundant in cells and have central roles in

protein-protein interaction networks. Many are involved in cancer, aging and

neurodegenerative diseases. The structure and dynamics of IDPs is intimately related to their

interactions with binding partners. Because IDPs are inherently flexible and do not have a

single conformation, conventional methods and conditions for determining structure and

dynamics of globular proteins may not be directly applicable. Nuclear magnetic resonance

(NMR) spectroscopy is one of the primary techniques characterizing the structures and

dynamics of IDPs, but one cannot rely solely on NMR data. A primary aim of this …

Physical Therapy Following Shoulder Rotator Cuff Repair, Ravindiran Appunni

Electronic Thesis and Dissertation Repository

ABSTRACT

Introduction

Rotator cuff (RC) tears are one of the common causes of pain and disability in the upper extremity. Currently there are no fixed guidelines for choosing testing positions for shoulder range of motion measurement. Optimal rehabilitation following RC repair is yet to be defined.

Purpose and Method

The purpose was to inform about postoperative Physical therapy following rotator cuff repair, with the following objectives:

• To systematically review the content of clinical research, which addresses various physical therapy programs.
• To describe validity and responsiveness of different testing positions for goniometric measurement of shoulder active external rotation.
• To pilot test …

Evaluating The Impact Of Two Different Methods Of Diabetes Self-Management Education On Knowledge, Attitude, And Behaviours Of Patients With Type 2 Diabetes Mellitus, Laura B. Briden

Electronic Thesis and Dissertation Repository

This study compared the effectiveness of diabetes self-management education (DSME) methods by examining changes in knowledge, attitude, and behaviour (KAB) after receiving education. Participants from a convenience sample were randomized into two groups, one receiving education through conversation maps and the other through traditional group education. Participants’ knowledge and attitude changes were measured by using a repeated measures pre-test/post-test design and changes in Hb A1c were observed. Focus groups were conducted after education was received to obtain perceptions and self-reported behaviour changes. Significant knowledge and attitude score changes were observed in the conversation map group after education. When comparing the …

Ethnic Identity And The Perception Of Facial Profile Aesthetics: A Preliminary Study, Paul Michael Glasser

Electronic Thesis and Dissertation Repository

In a multicultural society, the orthodontist is often faced with the challenge of interpreting an individual’s aesthetic preferences, which depending on the individual might differ from Eurocentric standards. Psychological researchers have recognized that ethnicity is dynamic, and better defined as a perception of self, influenced by social interaction and cultural involvement. Ethnic identity was investigated using three broad ethnic groups (Caucasian-American, African-American and Asian-American) with an established Multicultural Ethnic Identity Measure (Revised [MEIM-R]). Profiles for males and females of each group were constructed using published soft tissue data. Each profile was modified from subnasale to soft tissue B-point by displacing …

Characterization Of Cardiomyopathy In A Mouse Model Of Duchenne Muscular Dystrophy (Dmd) Using Echocardiography, Dce-Ct, And Pet-Fdg, Seyed Hamed Moazami

Electronic Thesis and Dissertation Repository

Duchenne muscular dystrophy (DMD) is an X-linked recessive neuromuscular disease that is the result of a loss of functional dystrophin, which causes cardiomyocyte fibrosis and death, leading to cardiomyopathy. In this thesis, I have utilized dynamic contrast-enhanced computed tomography (DCE-CT), positron emission tomography-fluorodeoxyglucose (PET-FDG), echocardiography, and traditional histology to longitudinally assess disease progression and degree of cardiomyopathy in a murine model of DMD (mdx:utrn-/-). No significant changes were observed in the blood flow, blood volume, or cardiac volume measured via DCE-CT, nor in standard uptake value (SUV) of glucose as measured by PET-FDG in the left myocardium between and within …

Social Marketing And Falls Prevention: Market Segmentation And Product Positioning, Eric C. Stemmler

Electronic Thesis and Dissertation Repository

The present study sets out to better understand how falls prevention can be marketed and positioned in order to increase participation rates in seniors. Market segmentation was used to create unique marketing mixes for individual groups of seniors. The senior market was segmented into frequent falling males and females, and non-falling males and females. Frequent fallers were defined by having experienced 2 or more falls in the past year, where non-fallers had experienced 1 or less falls. Twelve focus groups were conducted with thirty-five participants (N=35), 65 years or older. Differences were mainly found between number of falls …

Linking Psychological Capital, Structural Empowerment And Perceived Staffing Adequacy To New Graduate Nurses' Job Satisfaction, Lisa Stam

Electronic Thesis and Dissertation Repository

Reports indicate that new graduate nurses (NGNs) are experiencing stressful work environments, affecting job satisfaction and retention in current positions. New nurses are a health human resource that must be retained in order to ensure the replacement of retiring nurses, and to address impending shortages. As a result, creating supportive work environments that promote NGNs’ job satisfaction may play an important role in the retention and recruitment of skilled, satisfied nursing staff. The purpose of this study was to test the relationships between new graduates’ self-reported psychological capital (PsyCap), access to empowerment structures, perceptions of staffing adequacy and job satisfaction. …

Sexual Trafficking In The Canadian Context: Exploring The Political Landscape, Examining Discourse, And Identifying Health Issues Among Women With Lived Experience, Hoda Malakouti-Nejad

Electronic Thesis and Dissertation Repository

Although human trafficking has a long history, it has more recently become a topic of profound interest in popular culture, in political debates on sex work and immigration, and among law enforcement and social service providers. However, the widespread interest in human trafficking has not translated into a clear or consistent understanding of the phenomenon or the experiences of those who have been trafficked. This study sought to explore the perspectives of women who have been trafficked for sexual exploitation and the professionals who work with them. Specifically, the study examined three key issues in sexual trafficking: the political and …