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Full-Text Articles in Medicine and Health Sciences
The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida
The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida
The Thai Journal of Veterinary Medicine
The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa …
The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida
The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida
The Thai Journal of Veterinary Medicine
The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was …