Medicine and Health Sciences Commons^™

The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa …

The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was …

Childhood Non-Hodgkin's Lymphoma: An Immunophenotypic Analysis., Sajid Hussain Shah, Suhail Muzaffar, Shahid Pervez, Sheema H. Hassan

Department of Pathology and Laboratory Medicine

Objective: To observe the frequency of histological subtypes of childhood non-Hodgkin`s lymphoma and its immunohistochemical profile.

Setting: All cases of non-Hodgkin’s lymphoma diagnosed in children (<15 years) in the section of histopithology at the Aga Khan University Hospital Karachi during the period of three years.

Methods: These consecutive cases were evaluated on H&E stained sections and then immunohistochemistry analysis of these tumors was performed by employing Peroxidase Anti-Peroxidase (PAP) technique.

Results: The present series included 61 cases of non-Hodgkin’s lymphoma. NHL was more common in males as compared to females with male to female ratio of 5.8:1. High grade NHL comprised 87% of childhood lymphoma. The mode of presentation in majority of NHL (57%) was extranodal. Burkitt’s lymphonia (33%) …

Immunohistochemical Localization Of Cytokeratin, Vimentin And Alpa-Smooth Muscle Actin Micro And Intermediate Filaments In Canine Mammary Tumors, Ümi̇t H. Mi̇lli̇, Rifki Haziroğlu, Yilmaz Aydin, M. Yavuz Gülbahar

Turkish Journal of Veterinary & Animal Sciences

Forty-six formalin-fixed paraffin-embedded canine mammary tumors, 15 benign and 31 malignant, were studied immunohistochemically. Mouse monoclonal anti-alpha-cytokeratin 18, mouse monoclonal anti-alpha-cytokeratin (pan cytokeratin), mouse monoclonal anti-alpha-smooth muscle actin and mouse monoclonal anti-vimentin antibodies were used. The ductal and acinar epithelium stained positively for cytokeratin 18 and pan cytokeratin, although there was no immunoreactivity in some tumors. Metaplastic squamous cells in two solid carcinomas were positive for both cytokeratin 18 and pan cytokeratin. It was a notable finding that there were unreactive epithelial cells in some tumors for broad spectrum cytokeratin antibody (pan cytokeratin). Myofibroblasts in benign and malignant tumors were …

Epidermal Growth Factor Receptor (Egfr) Immunolocalization In The Male Rat Reproductive Tract During Pre-And Postnatal Periods, Celal Kaloğlu, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu

Turkish Journal of Veterinary & Animal Sciences

A number of recent studies suggest the effects of several growth factors as well as genetic factors on the development and differentiation of the testes and excretory ducts. One of the most important growth factors affecting the development and differentiation of the above-mentioned organs may be epidermal growth factor (EGF). Therefore, the aim of the present study was to investigate the effects of EGF on the development of the testes and excretory ducts of male rats by determining the immunolocalization of EGF receptors in these organs during foetal and postnatal periods. To do this, testicular and excretory duct tissue samples …