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Full-Text Articles in Medicine and Health Sciences

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Dec 2000

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida Dec 2000

The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa …


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2000

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Puppies Antibody Titre Response To Canine Distemper Virus Before And After Vaccination With A Cdv-Combined Vaccines, Woraporn Sukhumavasi, Boonlert Leephong, Silasak Kaewmaneerat, Suwit Gumtrontip, Monkon Trisiriroj, Pravina Kitikoon, Sumittra Watanodorn, Kanisak Oraveerakul Dec 2000

Puppies Antibody Titre Response To Canine Distemper Virus Before And After Vaccination With A Cdv-Combined Vaccines, Woraporn Sukhumavasi, Boonlert Leephong, Silasak Kaewmaneerat, Suwit Gumtrontip, Monkon Trisiriroj, Pravina Kitikoon, Sumittra Watanodorn, Kanisak Oraveerakul

The Thai Journal of Veterinary Medicine

Puppies antibody titer response to Canine Distemper virus (CDV) before and after vaccination with a CDV-combined vaccine was studied using a serum neutralization test (SN). A total of 201 samples were obtained from puppies at the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. The result showed that 100%(82/82) of the puppies, before vaccination, had no detectable SN titre (CDV SN-titer <2). Puppies 14 days after the first and second vaccination had a log2 (geometric mean titer, GMT) of 5.2 and 7.8. A group of 20 puppies in which the antibody response was followed after vaccination for a period of time showed the comparable results (log2 [GMT] = 5.3 and 8.5, respectively). There was no difference in the CDV SN-titers among puppies of different ages at vaccination, nor those with a vaccination history for their mothers (p>0.05).


What Is Your Diagnosis?, Pranee Tuntivanich Dec 2000

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Sds-Page Analysis For Protein Patterns Of Adults Haemonchus Placei, Mecistocirrus Digitalis And Cooperia Spp. In Cattle, Sonthaya Tiawsirisup, Amonthep Khuprathumsiri, Suwannee Nithiuthai, Nikom Chaisiri, Thirayuth Kositamongkol, Sudchit Chungpivat Sep 2000

Sds-Page Analysis For Protein Patterns Of Adults Haemonchus Placei, Mecistocirrus Digitalis And Cooperia Spp. In Cattle, Sonthaya Tiawsirisup, Amonthep Khuprathumsiri, Suwannee Nithiuthai, Nikom Chaisiri, Thirayuth Kositamongkol, Sudchit Chungpivat

The Thai Journal of Veterinary Medicine

Protein profiles of crude extracts from adult male and female Haemonchus placei, Mecistocirrus digitatus and Cooperia spp. were studied. The parasites were collected from the abomasum and duodenum of cattle at Pathumthani and Nonthaburi’s abattoirs. SDS-PAGE analysis of the crude extracts revealed, protein bands of various intensities ranging from 13- 24 bands with a molecular mass of between 19-130 kDa. The protein band patterns of each parasite at a molecular weight of between 48.2-111 kDa were similar whereas other protein bands at a molecular mass below 48.2 kDa were different. The number of distinct bands shown by the crude extract …


An Epidemiological Investigation Of Foot And Mouth Disease In A Dairy Farm In Uthaithani Province, Usuma Kukietnan, Suvut Chalajon Sep 2000

An Epidemiological Investigation Of Foot And Mouth Disease In A Dairy Farm In Uthaithani Province, Usuma Kukietnan, Suvut Chalajon

The Thai Journal of Veterinary Medicine

An epidemiological investigation of Foot and Mouth Disease (FMD) in Sawang Arom Dairy Co-operatives farm, between September-December 1998, found that 59 dairy farms holding 720 cattle, reported outbreak of the disease. Clinical examination revealed clinical signs in 65.55% of farms. Abortion (2.08%), stillbirth (0.83%) and mortality (0.83%) were found among the sick animals. The outbreaks were evident after introducing animals into farms where pregnant cows and calves were not vaccinated. Control of disease was achieved after 9 weeks following vaccination with trivalent- FMD vaccine (type O, A, Asia 1) which was given to all ≥ 3 mo. old calves and …


The Antimicrobial Resistance Of Salmonellae Isolated From Animals, Indhira Kramomtong, Channarong Rodkhum, Waree Niyomthum Sep 2000

The Antimicrobial Resistance Of Salmonellae Isolated From Animals, Indhira Kramomtong, Channarong Rodkhum, Waree Niyomthum

The Thai Journal of Veterinary Medicine

A total of 39 salmonella isolates were obtained from organ samples taken from duck, chicken and pig carcasses on 34 farms. They were tested for resistance to 11 antimicrobial drugs. 69.2% of isolates were resistant to at least one drug. Most resistances was shown to tetracycline and sulfamethoxazole while most isolates were susceptible to norfloxacin, enrofloxacin, flumiquine and furazolidone. Ampicillin and cephapirin were effective on the salmonella isolates from ducks and chicken, whereas kanamycin was effective on the isolates from chicken and pigs. Trimethoprim was the drug of choice on the isolates from ducks. Forty eight percent of the isolates …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Sep 2000

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich Sep 2000

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Serum Cholinesterase Levels In Elephants, Piyarat Subhachalat, Wara Panichkriangkrai, Sittidet Mahasawangkul, Taweepok Angkawanich, Sirintorn Yibchok-A-Nun Sep 2000

Serum Cholinesterase Levels In Elephants, Piyarat Subhachalat, Wara Panichkriangkrai, Sittidet Mahasawangkul, Taweepok Angkawanich, Sirintorn Yibchok-A-Nun

The Thai Journal of Veterinary Medicine

Serum cholinesterase (ChE) levels were determined in adult elephants in the summer, the rainy season and in the winter by a colorimetric method using acetylthiocholine as the substrate. The results showed that ChE levels in adult males were lower than those in adult females, in all three seasons (p < 0.05). Furthermore, the ChE level was at it’s highest in the rainy season, falling lower inมthe summer and lower again in the winter season. The difference was found in both sexes. This study is the first report on the measurement of ChE levels in elephants.


An Outbreak Of Leptospirosis In A Swine Breeding Herd : It's Diagnosis, Treatment And Economic Effect, Duangjai Suwanchareon, Annop Kunavongkrit Sep 2000

An Outbreak Of Leptospirosis In A Swine Breeding Herd : It's Diagnosis, Treatment And Economic Effect, Duangjai Suwanchareon, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

An outbreak of leptospirosis in a 2600 sow breeding herd, in which 5% of the sows aborted, was investigated. Serum samples from 11 aborted sows were examined for leptospira antibodies. Using a Microscopic Agglutination Test (MAT), containing only 12 serovars, no leptospira serovars were detected. However, after using a Microcapsule Agglutination Test (MCAT) kit, a further MAT using the additional three serovars (sejroe, hardjo and bratislava) revealed that the cause of abortion was Leptospira interrogan serovars bratislava and sejroe. After the final diagnosis, the animals were treated by using 800 ppm chlortetracycline in the feed for two weeks, followed by …


นัยน์ตาและการตรวจตา, Pranee Tuntivanich, Nalinee Tuntivanich Sep 2000

นัยน์ตาและการตรวจตา, Pranee Tuntivanich, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Sep 2000

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Artificial Breeding Of Large Ruminant Production : Based On Experiences In Thailand, Maneewan Kamonpatana Jun 2000

Artificial Breeding Of Large Ruminant Production : Based On Experiences In Thailand, Maneewan Kamonpatana

The Thai Journal of Veterinary Medicine

The author has been much concentrating on biological regulation for enhancing fertility and growth with co-incidentally better health care. The three aspects of achievement of fertility, growth and health within one injection may be called “Three in One that is Mato-Matid technology”. However, Mato-Matid technology did provide a great deal of prime biological responses positively to several reproductive technologies in which some of them may be regarded as artificial breeding like IVM/IVF/IVC/ET/Cloning.


Canine Extraskeletal Osteosarcoma Of The Salivary Gland, Theerayuth Kaewamatawong, Anudep Rungsipipat, Suwicha Chutatep Jun 2000

Canine Extraskeletal Osteosarcoma Of The Salivary Gland, Theerayuth Kaewamatawong, Anudep Rungsipipat, Suwicha Chutatep

The Thai Journal of Veterinary Medicine

An 8 year-old male mongrel dog and 12 year-old male German Shepherd had a 10 x 10 cm mass and 15 x 10 cm mass located in the mandibular region. The encapsulated firm white masses had multiple cysticlike nodules filled with mucous. Histopathology revealed clusters of pleomorphic, large, round to oval shaped cells, irregular ovoid to spindle nuclei with highly mitotic figures that showed osteoid and bony formation. The neoplastic cells were immunoreactive in some parts for vimentin and cytokeratin but not for desmin, α-SMA and S-100 protein. Hyperplastic salivary gland cells were also present. Electron microscopy revealed prominent nucleoli …


The Histological Structure Of Testicular And Male Genital Ducts In Siamese Crocodiles (Crocodylus Siamensis), Suppaluk Roomratanapun, Somphop Navephap, Natanun Makmee Jun 2000

The Histological Structure Of Testicular And Male Genital Ducts In Siamese Crocodiles (Crocodylus Siamensis), Suppaluk Roomratanapun, Somphop Navephap, Natanun Makmee

The Thai Journal of Veterinary Medicine

Five sets of testes and male genital ducts of Siamese crocodiles, aged 3-4 years were collected and processed by the histologic technique. The tissues were cut 5 micrometres thick and stained with H&E, PAS and Masson’s trichrome methods. The sections were observed under a light microscopic. The testes were surrounded by fibrous connective tissue, with the tunica albuginea and their septum separating the organs into lobules. The testes consisted of packed seminiferous tubules ; the lumen of tubules consisted of various stages of spermatogenic and Sertoli cells. The latter were irregularly shaped with ovoidal or pyramidal nuclei and a prominent …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Jun 2000

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


Ultrasound Diagnosis, Phiwipha Kamonrat Jun 2000

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich Jun 2000

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Pathological Changes And Faecal Excretion Levels Seen In Broiler Chicks After A Single Oral Dose Of Aflatoxin B1, Kanarat Harintharanon, Achariya Sailasuta, Anudep Rungsipipat, Anong Bintvihok, Jiroj Sasipreyajan Jun 2000

Pathological Changes And Faecal Excretion Levels Seen In Broiler Chicks After A Single Oral Dose Of Aflatoxin B1, Kanarat Harintharanon, Achariya Sailasuta, Anudep Rungsipipat, Anong Bintvihok, Jiroj Sasipreyajan

The Thai Journal of Veterinary Medicine

Three hundred Shaver starbo, broiler, 5-day-old chicks were divided into 3 groups and given in group 1 sterile water, group 2 olive oil and group 3 aflatoxin B1. The effect of aflatoxin B1(5mg/kg) after the single oral dose was studied, observing clinical signs, blood chemistry, pathology, and excretion levels in faeces over a period of 10 days. Chicks in group 3 showed depression and decreased feed intake. Blood chemistry, especially blood urea nitrogen (BUN) was significantly different between the three groups although still in the normal range. Gross pathology revealed petechial hemorrhages developing on day 2 (87.5 %) and pale …


Artificial Insemination Research Developments In The Pig, Annop Kunavongkrit Mar 2000

Artificial Insemination Research Developments In The Pig, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

Artificial Insemination (AI) in pigs has been well developed especially in the area of instrument design and semen preservation. AI techniques normally practiced as a routine, work well in Thailand swine breeding farms. Current research for the development of AI in pigs at the Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University consists of: fractionated insemination, semen evaluation testing the ability of sperms to penetration oocytes and fertilise and the economic evaluation of AI boars raised in evaporative cooling systems. These researches have been reviewed in this report.


The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida Mar 2000

The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was …


Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol Mar 2000

Embryo And Oocyte Collection In Thai Swamp Buffalo (Bubalus Bubalis) After Superovulation, Mongkol Techakumphu, Yant Sukwong, Malee Apimeteetumrong, Siriwat Intaramongkol, Jintana Intaramongkol

The Thai Journal of Veterinary Medicine

Six-day embryos were recovered from 25 swamp buffalo cows treated with pregnant mare serum gonadotropin (2,500 iu PMSG, n=9) and follicle stimulating hormone (280 mg, n=12 and 400 mg FSH, n=4). Two techniques, non surgical collection from live animals and direct flushing after slaughter were compared. The oocytes were collected from ovaries immediately after slaughter by aspiration with or without ovarian chopping. The studies showed that 0.44+0.59 embryos were collected from PMSG group, while 1.38+1.31 embryos were collected in FSH group. Fifty percent to 60% of recovered embryos were transferable. The non surgical technique gave a low recovery rate (14.40%) …


The Subdural Sinus Of The Freshwater Crocodile (Crocodylus Siamensis), Wimon Pothiwong, Pakorn Prachammuang, Weerapong Koykul Mar 2000

The Subdural Sinus Of The Freshwater Crocodile (Crocodylus Siamensis), Wimon Pothiwong, Pakorn Prachammuang, Weerapong Koykul

The Thai Journal of Veterinary Medicine

A blood sinus suitable for the collection of blood was studied in four freshwater crocodiles (Crocodylus siamensis). The sinus was located in the neck between the dura mater and the archnoid, which gave it its name "the subdural sinus". The procedure of blood collection in crocodiles is discussed in terms of possible injury to the spinal cord and the chance of getting cerebrospinal fluid mixed into the blood sample.


Ultrasound Diagnosis, Phiwipha Kamonrat Mar 2000

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Mar 2000

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


A Preliminary Study On Oocyte Collection Using Transvaginal Ultrasound In Swamp Buffalo Heifers (Bubalas Bubalis), Akachart Promdireg, Mongkol Techakumphu, Nawapen Phutikanit, Ancharlie Na-Chiangmai Mar 2000

A Preliminary Study On Oocyte Collection Using Transvaginal Ultrasound In Swamp Buffalo Heifers (Bubalas Bubalis), Akachart Promdireg, Mongkol Techakumphu, Nawapen Phutikanit, Ancharlie Na-Chiangmai

The Thai Journal of Veterinary Medicine

The objective of the study was to investigate the possibility of collecting oocytes using transvaginal ultrasound-guidance, in swamp buffalo heifers, after gonadotropin stimulation. Five swamp buffalo heifers were given a single dose of 3,000 IU of Pregnant Mare Serum Gonadotropin (PMSG) intramuscularly in order to stimulate the ovaries. Examination of the ovarian responses were carried out 48h after stimulation, the follicles were subsequently punctured and the oocytes examine under a stereomicroscope. The results of the ovarian response were 6.6 ± 1.1 follicles per animal with an average diameter of 7.1 ± 4.1 mm. The mean number of oocytes collected per …


What Is Your Diagnosis?, Pranee Tuntivanich Mar 2000

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.