Medicine and Health Sciences Commons^™

A Potential Macroregulatory Mechanism For Growth Regulation In Neurospora Crassa, Martha Mooney

Biological Sciences Theses & Dissertations

Neurospora produces a phase specific, cationic mucopolysaccharide composed primarily of galactosamine (GAG-MP) which becomes part of the cell wall and is later secreted into the media. Its appearance coincides with the onset of the restricted phase of growth and causes efflux of small molecular weight metabolites when incubated with conidial cells. This activity may be a product of electrostatic interactions of the GAG-MP with the conidial plasma membrane. The activity is blocked by acetylation of the primary amines on the molecule, digestion by enzymes that hydrolyze carbohydrate linkages and the inclusion of NaCl (lM) in the GAG-MP/conidia reaction mixture. The …

Life Goals Of Five Dental Hygiene Groups In Three Educational Settings, Kathleen Olivia Hodges

Dental Hygiene Theses & Dissertations

This study investigated life goals of dental hygiene students and 1978., 1976, 1975, and 1974 graduates in three different educational settings.

The California Life Goals Evaluation Schedule was used to measure life goals of dental hygienists attending, or who had attended, Cuyahoga Community College· in Ohio, Idaho State University, and Old Dominion University in Virginia.

An ex post facto 5x3 research design was used. Year of graduation and educational setting were the nonmanipulated independent variables; ten scores on the California Life Goals Evaluation Schedule were the dependent variables; and age was used as a covariant.

Two-way multivariant analysis yielded no …

Values Of Dental Hygienists In Three Different Occupational Settings, Adele Cereghino Koot

Dental Hygiene Theses & Dissertations

This study investigated values of dental hygienists in three occupational settings: private practice, community practice, and higher education.

The Allport, Vernon, Lindzey Study of Values was used to measure values of all licensed dental hygienists in North Carolina employed in one of the three occupational settings.

An ex post facto 3x3x3x4x4 factorial research design was used. Occupational setting, age, year of graduation, educational level, and length of time in present position were the non-manipulated independent variables; and the Allport, Vernon, Lindzey Study of Values scores were used as dependent variables. Five-way analysis of variance revealed no significant value differences among …

Creativity In Dental Hygiene Students And Dental Hygiene Professionals, Frances Lynne Jarrett

Dental Hygiene Theses & Dissertations

The purpose of this investigation was to examine creativity in dental hygiene students, dental hygiene educators, and dental hygiene practitioners.

Torrance's creativity test, What Kind of Person Are You?, was used to measure creativity in a convenience sample of 231 subjects. Data were organized according to ex post facto research design utilizing educational and occupational status as the non-manipulated independent ,, variables, and What Kind of Person Are You? creativity scores as the dependent variables. The statistical tests, analysis of variance and chi-square were employed to analyze data for significant differences between and among the mean scores of all sample …

Demonstration Of Unique Mycelial Components In Candida Albicans, David Jon Mancuso

Biological Sciences Theses & Dissertations

Mycelial (M) and blastopore (B) cell homogenates of Candida albicans strain BJ85 were compared to detect and recover unique M components. Qualitative differences in the protein and lipid content of Mand B homogenates were suggested by discontinuous polyacrylamide gel electrophoresis (DPAGE). In addition, a quantitative and possibly qualitative shift in the protein content during germ tube formation was implied by DPAGE. Unique M components were detected using both DPAGE and immunoelectrophoretic (IE) techniques. One unique M component by DPAGE, four by IE, and two by combined DPAGE and IE techniques were suggested. Of these three methods, only DPAGE and the …

The Effects Of Oral Health In-Service Education In An Extended Care Facility, Marcia Brand Doloresco

Dental Hygiene Theses & Dissertations

A study was conducted to determine the effects of two oral health in-service teaching methodologies on intact groups of staff members and patients at two extended health care facilities. Oral health in-service education conducted by a dental hygienist (IV₁ ); and oral health in-service education with direct guidance of the staff's oral health services to patients by the dental hygienist (IV₂ ) were implemented at the Medicenters of Virginia Beach and Norfolk, respectively.

Base line and post evaluation data on the oral health status of each patient group were collected utilizing the Simplified Oral Hygiene Index. Base line …

Duck Plague Virus An Investigation Of Cytopathic Effects, In-Vitro Host Range, And Inhibition By Phosphonoacetic Acid, Ronald Attanasio

Biological Sciences Theses & Dissertations

Duck plague is a hemorrhagic, necrotic disease found exclusively in the order Anseriformes, family Anatidae (ducks, geese, swans). The etiologic agent has been reported to be a herpes-like virus. The Holland strain of duck plague was found to induce cytopathic effects in cell culture (perinuclear vacuolation followed by lysis). A simple reproducible plaque assay for quantitating the virus was developed. Under conditions of the plaque assay, one-hit kinetics were observed. The host range of duck plague virus in cell culture was found to include the order Galliformes, family Phasianidae (pheasants, quails and partridges). Duck plague virus-induced cell culture cytopathic effects …

Kinetic And Binding Studies On L-∝-Glycerophosphate Dehydrogenase, Paul Richard Rosevear

Chemistry & Biochemistry Theses & Dissertations

The properties of the coenzyme, NAD, binding site of chicken muscle L-∝-glycerophosphate dehydrogenase were studied. Adenosine monophosphate, adenosine diphosphate and adenosine diphosphoribose were sha.vn to inhibit the enzyme competitively with respect to NAD. The presence of adenosine, pyrophosphate and ribose regions in the coenzyme binding site of the enzyme was suggested by the inhibitor constants obtained for these compounds.

Disodium monoalkyl phosphates, n-butyl to n-dodecyl phosphate, inclusive, were also shown to inhibit the L-∝-glycerophosphate dehydrogenase catalized reaction competitively with respect to NAD. A positive chain length effect was observed in the binding of these compounds to the enzyme, suggesting the …

The Pathophysiology Of Acute Venous Occlusion, Lewis H. Curd Jr.

Biological Sciences Theses & Dissertations

Large artery, pedal small vein, femoral vein and central venous pressures and extremity volume changes were recorded in mongrel dogs in which the hindlimb was perfused at a constant flow. Arterial, pedal venous and femoral venous blood gases were drawn and analyzed for measurements of arterio-venous O₂ and CO₂ differences. Paw and thigh resistances were calculated. Measurements were repeated following ligation of the iliac and femoral veins, alpha receptor blockade (dibenzyline), and dilation with Vasodilan. Results indicate that ligation of extremity veins results in an active ·increase in total limb resistance below the ligation, an increase in the …

A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell

Chemistry & Biochemistry Theses & Dissertations

A method is described for preparing an enriched fraction of the three major antibodies, immunoglobulins IgG, IgA and IgM, from normal human serum by a batch method. Selective precipitation of these proteins was carried out in successive steps using polyethylene glycol (PEG) of average molecular weight 6,000 at concentrations of 7%, 12% and 14% (16) . Zinc sulfate (10 mM) was used to remove highly immunogenic alpha₂ macroglobulin from the IgM fraction. IgA, IgM and IgG were further purified by adsorption methods using DEAE-cellulose. Quantitation and qualitation of the enriched fractions showed nearly complete separation of each of the …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Seasonal Distribution Of Higher Marine And Aquatic Fungi In The Back Bay National Wildlife Refuge, Scott Schatz

Biological Sciences Theses & Dissertations

Abstract unavailable.

Comparison Of Interferon Produced In Vitro By Monkey Kidney Cells Demonstrating Interference Against Polio Ii, Coxsackie A-9, Influenza A2/Hong Kong/68, And Echo-9 Viruses, Archer Duncan Farmer Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable

Phytoplankton Succession In The Lafayette River, Estuary, Norfolk, Virginia, Thomas W. Purcell Lll

Biological Sciences Theses & Dissertations

Abstract unavailable.

A Study Of Precipitin And Soluble Antigen Fluorescent Antibody Technique In The Detection Of Dirofilaria Immitis In Canines, John H. Ellsworth

Biological Sciences Theses & Dissertations

Abstract unavailable.