Medicine and Health Sciences Commons^™

Two Factors That Bind To Highly Conserved Sequences In Mammalian Type C Retroviral Enhancers., Nancy R. Manley, Mary M. O'Connell, Wanwen Sun, Nancy A. Speck, Nancy Hopkins

Dartmouth Scholarship

The transcriptional enhancers of the Moloney and Friend murine leukemia viruses (MLV) are important determinants of viral pathogenicity. We used electrophoretic mobility shift and methylation interference assays to study nuclear factors which bind to a region of these enhancers whose sequence is identical between Moloney and Friend viruses and particularly highly conserved among 35 mammalian type C retroviruses whose enhancer sequences have been aligned (E. Golemis, N. A. Speck, and N. Hopkins, J. Virol. 64:534-542, 1990). Previous studies identified sites for the leukemia virus factor b (LVb) and core proteins in this region (N. A. Speck and D. Baltimore, Mol. …

Characterization Of A Protein That Binds Multiple Sequences In Mammalian Type C Retrovirus Enhancers., Wanwen Sun, Mary M. O'Connell, Nancy A. Speck

Dartmouth Scholarship

Mammalian type C retrovirus enhancer factor 1 (MCREF-1) is a nuclear protein that binds several directly repeated sequences (CNGGN6CNGG) in the Moloney and Friend murine leukemia virus (MLV) enhancers (N. R. Manley, M. O'Connell, W. Sun, N. A. Speck, and N. Hopkins, J. Virol. 67:1967-1975, 1993). In this paper, we describe the partial purification of MCREF-1 from calf thymus nuclei and further characterize the binding properties of MCREF-1. MCREF-1 binds four sites in the Moloney MLV enhancer and three sites in the Friend MLV enhancer. Ethylation interference analysis suggests that the MCREF-1 binding site spans two adjacent minor grooves of …

Longitudinal Study Of Plasma And Erythrocyte Selenium Levels In Type I Diabetic Children During Development And Treatment Of Ketoacidosis, Pi-Hsia Fan

Chemistry & Biochemistry Theses & Dissertations

Most studies on the biological activity of selenium have focused on its role at the active site in the ubiquitous enzyme, glutathione peroxidase (GSH-Px) which catalyzes the removal of hydrogen peroxide and organic hydroperoxides (1). A connection between selenium deficiency and a wide range of diseases in animals and humans has been reviewed (2). Development of various forms of human cancer (3), failure of the immune system (4), as well as some cardiovascular diseases (5) have been reported or suspected to correlate with selenium deficiency. However, the physiological function of selenium in these different diseases is not fully understood, and …

[Introduction] The Science Court Is Dead - Long Live The Science Court, Thomas G. Field

RISK: Health, Safety & Environment (1990-2002)

[Excerpt] "It is a pleasure to introduce this symposium issue with its range of current thoughts about what Arthur Kantrowitz invented a little over twenty-five years ago and has since come to be known as the "Science Court." The pleasure is enhanced by being able to include papers by Dr. Kantrowitz, Allan Mazur (who worked closely with him), Carl Cranor, Itzhak Jacoby and Sheila Jasanoff - as well as an extensive list of citations to other discussions. In approaching these papers, readers may find it helpful to consider what Kantrowitz invented, he and others have attempted to improve, and the …

Human Performance Lab Newsletter, February 1993, St. Cloud State University

Human Performance Lab Newsletter

Contents of this issue include:

• A Lesson from Grandma by Moira Petit
• Two Steps to Better Health by Jack Kelly
• Home Exercise Equipment: What's Right for Me? by Kari Brown
• Quality Quenching
• Just a Reminder (on frostbite) by Mike Reid
• Stress Buster by Mary Kazemba
• Nutrition Update by Lori Hyink
• HPL Research
• Graduate Programs & Research

Treat It Right : Alternative Wastewater Systems That Protect Water Quality, Brian Kent

Maine Collection

Treat It Right : Alternative Wastewater Systems That Protect Water Quality

Maine Dept. of Environmental Protection : Maine Dept. of Economic & Community Development, February 1993.

"This handbook was prepared by Maine Tomorrow, a consulting firm in Hallowell. Maine. The handbook was researched, written and designed by Brian Kent; the primary editor and research associate was Alison Truesdale, and Wendy Mahoney did the wordprocessing and desk-top printing."

Ua12/12 Your Healthstyle, Wku Wellness Center

WKU Archives Records

Newsletter created by the Wellness Center regarding services, health and fitness.

Restaurant Dining Strategies: Attracting Nutrition-Conscious Future Seniors, Elizabeth B. Pederson, Frederick J. Demicco

Hospitality Review

Senior Customers pose some unique challenges to operators due to some of the physiological changes associated with aging. In an effort to make food and beverage managers more cognizant of these changes, the authors examine these areas and also discuss strategies to attract and enhance the dining experience of the viable senior market segment.

Detection Of Serum Antibody Responses In Cattle With Natural Or Experimental Neospora Infections, Patricia Conrad, Karen Sverlow, Mark Anderson, Joan Rowe, Robert Bondurant, Gwen Tuter, Richard Breitmeyer, California Department Of Food And Agriculture, Animal Health Branch, Mark Thurmond, Alex Ardans, J. P. Dubey, Gerarld Duhamel, Bradd Barr

School of Veterinary and Biomedical Sciences: Faculty Publications

Parasite-specific antibody responses were detected using an indirect fluorescent antibody (IFA) test in cattle that were naturally or experimentally infected with Neospora parasites. The test was developed using Neospora tachyzoites isolated from an aborted bovine fetus and grown in bovine cell cultures (isolate BPA1). In all cases, infections were confirmed by the identification of Neospora tachyzoites and/or bradyzoite cysts in fetal or calf tissues using an immunoperoxidase test procedure. Fifty-five naturally infected cows that aborted Neospora-infected fetuses had titers of 320-5,120 at the time of abortion. The titer of 6 cows that were serologically monitored over a prolonged period decreased …

Choosing Shower Dips For Sheep Lice, Tony Higgs, Bob Love

Journal of the Department of Agriculture, Western Australia, Series 4

The performance of all shower dip chemicals for sheep lice can vary depending on the active chemical and the conditions under which they are used.

Department of Agriculture trials have indicated that the wettable powders coumaphos and magnesium fluorosilicate were less effective at eradicating sheep lice than were synthetic pyrethroid and organophosphate dipping chemicals.

However, failure to eradicate sheep lice may be the result of several factors, many of them related to management.

Do Farmers Really Want To Eradicate Sheep Lice?, Chris Hawkins

Journal of the Department of Agriculture, Western Australia, Series 4

With the termination of Western Australia's Sheep Lice Eradication Campaign in 1993 farmers' views about the eradication of sheep lice are of greater relevance now than in the past. Lice control now rests with individual farmers and continuing local lice cell groups .

A recent survey of producers in the Moora Shire provides some key insights into farmers' thoughts about sheep lice and their eradication.

Heterophil Chemotaxis In Chickens With Natural Staphylococcal Infections, James R. Andreasen Jr., Claire B. Andreasen, Mohammad Anwer, Anita E. Sonn

Claire B. Andreasen

Heterophil chemotaxis using heterophils isolated from the peripheral blood of five commercial broiler chickens naturally infected with staphylococcal bacteria was compared by the modified Boyden-chamber technique with chemotaxis of heterophils from two chickens from the same flock not infected with Staphylococcus (field controls) and from four healthy laboratory control broiler chickens. The infected chickens had gross and histologic lesions of staphylococcal tenosynovitis and osteomyelitis. Staphylococci were isolated from the lesions. Hematologic parameters and histologic lesions of infected chickens also were examined. Compared with field and laboratory controls, Staphylococcus-infected chickens had heterophilic leukocytosis. The heterophils of Staphylococcus-infected chickens had significantly lower …

Chicken Heterophil Chemotaxis Using Staphylococcus-Generated Chemoattractants, James R. Andreasen Jr., Claire B. Andreasen, Mohammad Anwer, Anita E. Sonn

Claire B. Andreasen

Heterophil chemotaxis, in response to chemotactic factors generated by three different strains of staphylococcal bacteria, was measured using the modified Boyden-chamber technique. Heterophils were obtained from healthy 6-to-8-week-old broiler chickens. Each bacterial strain generated factors that were chemotactic for chicken heterophils. Factors generated by two pathogenic isolates of Staphylococcus aureus, however, induced significantly greater chemotaxis in chicken heterophils than those generated by a nonpathogenic Staphylococcus isolate.

Ua66/14/4 Kentucky Partnership Newsletter, Vol. 1, No. 1, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Epidermal Mosaicism And Blaschko's Lines, Celia Moss, S. Larkins, Michael W. Stacey, A. Blight, P. A. Farndon, E. V. Davidson

Bioelectrics Publications

To test the hypothesis that epidermal rather than dermal mosaicism determines Blaschko's lines in hypomelanosis of Ito (HI), we studied the distribution of chromosomal mosaicism in four patients. In two, mosaicism had not been detected in lymphocytes or dermal fibroblasts, but was clearly shown in epidermal keratinocytes; furthermore, the abnormal cell line was confirmed to the hypopigmented epidermis and the normal epidermis contained only normal cells. Negative findings in the other two patients might be because of mosaicism which was undetected either because it was submicroscopic or because it was present in melanocytes, which have not yet been studied. These …

1993 Beef Cattle Report

Nebraska Beef Cattle Reports

Cow-Calf • Forage Systems Using Sandhills Subirrigated Meadows and Range: Beef Production Traits • Forage Systems Using Sandhills Subirrigated Meadows and Range: Economic Analysis • High Quality Meadow Hay as a Winter Supplement for Gestating Beef Cows in the Sandhills of Nebraska • Heifers Sired by Bulls with Either High or Low EPD's for Marbling Do Not Differ in Age at Puberty • Synovex C Affects Growth, Reproduction, and Calving in Heifers • Synthetic Progestins Influence Secretion of Luteinizing and Timing of Ovulation • Level of Progesterone Influences Pregnancy Rate in Beef Cows and Heifers • Combination of Factors (Ratios) …

Book Review: The Baculovirus Expression System: A Laboratory Guide (1992) King, L. A. & Possee, R. D., David D. Dunigan

Nebraska Center for Virology: Faculty Publications

The power of molecular biology is unleashed with the ability to clone and sequence genes, and then express these genes in heterologous systems. This sets the stage for the full analysis of proteins that are otherwise difficult to isolate and/or purify, especially when present at very low copy number per cell or when isolated from relatively precious materials. Overexpression of protein is now possible in a number of systems including prokaryotes (e.g., E. coli) and various eukaryotes (yeast, insects, and plants). The issue then becomes, which system (1) most closely reflects the homologous expression with respect to posttranslational modifications, …

Salmonella Hadar Pericarditis, A Aziz, W Jafri, T A. Jawed, A Shaikh, B Farooqui, M K. Ashfaq, N Ul Haq

Department of Pathology and Laboratory Medicine

No abstract provided.

Detection Of Point Mutations In The Dystrophin Gene, John Pedretti

Theses : Honours

The dystrophin gene has been localised to Xp 21.1. Mutations of this gene can lead to the clinical manifestations of Duchenne and Becker muscular dystrophies (DMD/BMD). In the majority of DMD and BMD patients the disease-causing mutation is a deletion detectable by southern analysis or multiplex PCR, however in 30% of patients no deletion is observed using these conventional tests. Using PCR amplification of cDNA it was possible to detect a deletion in the product of the dystrophin gene of one such individual affected with BMD. It was then necessary to characterise the mutation in order to determine whether this …

Studies On The Oyster Pathogen Perkinsus Marinus (Apicomplexa): Interactions With Host Defenses Of Crassostrea Virginica And Crassostrea Gigas, And In Vitro Propagation, Jerome F. La Peyre

Dissertations, Theses, and Masters Projects

The disease caused by the protozoan Perkinsus marinus has been a major source of mortality in the eastern oyster, Crassostrea virginica. Variations in susceptibility to P. marinus infection among eastern oysters collected from the Chesapeake Bay and Gulf of Mexico, as well as between eastern and Pacific (Crassostrea gigas) oysters were determined. Since oyster host defense may play a role in determining susceptibility to pathogen infection, cellular and humoral defense activities of the oyster and their interactions with P. marinus were investigated. Procedures also had to be established to isolate, purify, and propagate in vitro, P. marinus. Eastern oysters from …

Role Of Endophytes In Tall Fescue, E. L. Piper, C. P. West

Journal of the Arkansas Academy of Science

Tall fescue (Festuca arundinacea Schreb.) is the most commonly grown cool season grass used for pastures in Arkansas. Most tall fescue contains a fungal endophyte (Acremonium coenophialum Morgan-Jones & Gams), which causes fescue toxicosis in livestock and costs cattle producers millions of dollars annually in lost production. Endophyte presence is known to reduce wild mammal populations in areas where tall fescue is prevalent. The endophyte spends its entire life cycle within the plant and is transmitted through the seed. The association is mutualistic with the plant providing nutrients for the endophyte and the endophyte conferring drought, insect, and nematode resistance …

Model Consent Forms For Dna Linkage Analysis And Storage, Roger B. Dworkin, R. L. Gold, R. R. Lebel, E. A. Mearns, T Hadro, J. K. Burns

Articles by Maurer Faculty

No abstract provided.

Crime, Race And Reproduction, Dorothy E. Roberts

All Faculty Scholarship

No abstract provided.

Cysticercosis In A Muslim Woman, Farrukh Aijaz, Altaf Ahmed

Department of Pathology and Laboratory Medicine

No abstract provided.

Alternatives In The 90'S: What's Next?, Henry Spira

Conference Presentations

The 80's was a decade of remarkable and innovative research and development of alternatives. We see the 90’s as the decade of validation and implementation.

From an activist's perspective, the 80's was very encouraging,-- alternatives gained acceptance, legitimacy and credibility within the toxicology, corporate and regulatory communities. And The Center for Alternatives to Animal Testing (CAAT) at Johns Hopkins University played a significant role in making this acceptance possible.

But these new technologies have yet to realize their full potential; the focus to date has been on development more than on bringing these technologies into routine usage. And the public, …

Progress Toward Eradication Of Virulent Footrot, Bob Mitchell

Journal of the Department of Agriculture, Western Australia, Series 4

Western Australia is well placed to eradicate virulent footrot from the State's sheep and goat populations.

Between 1950 and 1993 the proportion of sheep flocks with footrot fell from about 15 per cent to less than 2 percent, and the 1990-92 outbreak has been turned around. One hundred and eighty properties (]. 7 per cent) of flocks, mostly in the high rainfall South-West, are in quarantine for footrot today.

This high level of footrot control is the result of strong support from the sheep industry and years of good cooperation between farmers and the Department of Agriculture.

Western Australia leads …

The Distribution And Occurrence Of Aerobic Heterotrophic Antimicrobic-Producing Bacteria Isolated From The Lower Chesapeake Bay, Linda Lea Gilmer

Biological Sciences Theses & Dissertations

Three different estuarine microenvironments in the lower Chesapeake Bay were investigated for the occurrence and geographical distribution of antimicrobic-producing bacteria. Samples were taken from the water column, fish skin, and algal surfaces. A total of 1472 bacterial isolates were assayed for antimicrobial activity against fungal, Gram-negative and Gram-positive bacterial test organisms. Of the total bacterial isolates assayed, 5. 2% were active antimicrobic producers. Algal surf aces were shown to have a higher incidence of antimicrobic-producing bacteria than the water column or fish skin. No antimicrobic activity was demonstrated by bacteria isolated from the flounder fish skin. The majority of the …