Medicine and Health Sciences Commons^™

Electrophysiology, Cell Calcium, And Mechanisms Of Hepatocyte Volume Regulation, Walid E. Khalbuss

Electronic Theses and Dissertations

The electrophysiologic technique (Reuss, L., Proc. Natl. Acad. Sci. USA 82:6014, 1985) was modified to measure changes in steady-state hepatocyte volume during osmotic stress. Hepatocytes in mouse liver slices were loaded with tetramethylammonium ion (TMA$\sp{+}$) during transient exposure of cells to nystatin. Intracellular TMA$\sp{+}$ activity (a$\sp{\rm i}\sb{\rm TMA}$) was measured with TMA$\sp{+}$-sensitive, double-barreled microelectrodes. Loading hepatocytes with TMA$\sp{+}$ did not change their membrane potential (V$\sb{\rm m}$), and under steady-state conditions a$\sp{\rm i}\sb{\rm TMA}$ remained constant over 4 min in single impalements. Hyperosmotic solutions (50, 100, & 150 mM sucrose added to media) and hyposmotic solutions (sucrose in media reduced by …

The Effects Of Aerobic Conditioning Upon The Resting Metabolic Rate, Marti R. Davis

Masters Theses

This study com pared the metabolic rate following an exercise bout to the resting metabolic rate (RM R) established in pretesting procedures for twelve college age females. The only factor differentiating the groups was length o f the training period (one week, two weeks, four weeks, and five weeks). Training took place on a Bosch cycle ergometer three times a week, for 30 minutes each session, at 70 to 85 percent o f the subjects' heart rate reserve (HRR). Breath analysis was measured on a Beckman Metabolic Cart in both pretesting and post-testing procedures.

The findings indicated that aerobic exercise, …

Morphological And Physiological Examination Of A Demyelinating And Remyelinating Lesion, Paul Anson Felts

Theses and Dissertations in Biomedical Sciences

Multiple sclerosis causes demyelination of central nerve fibers, and it is this pathology which results in most of the symptoms of the disease. The repair of the fibers by remyelination has been advanced as a potential symptomatic therapy, but at present the conduction properties of remyelinated central fibers are not well understood. In this study the conduction properties of Schwannian or oligodendrocyte remyelinated central nerve fibers have been determined. In addition, the status of the blood-brain barrier has been examined in lesions characterized by Schwann cell remyelination. These lesions chronically lack astrocytes, a cell believed to be involved in the …

An Evaluation Of Cells Recruited To Ectopically Implanted Bone Matrix, Janel Dvonch Kelly

Dissertations

No abstract provided.

Anatomical And Neurochemical Organization Of Medullary And Parabrachial Efferents To The Amygdala In The Rat, Andrea M. Zardetto-Smith

Dissertations

No abstract provided.

Ultrastructural And Invasive Characteristics Of Murine B16a Melanoma Cells: An In Vitro And In Vivo Study, Terence William Mcgarvey

Dissertations

No abstract provided.

Effects Of Sodium Intake, Furosemide, And Infusion Of Atrial Natriuretic Peptide On The Urinary And Metabolic Clearances Of Arginine Vasopressin In Normal Subjects, Arnold M. Moses, Carol Jones, Carolyn B. Yucha

Nursing Faculty Publications

Arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) have important influences on water and electrolyte metabolism, and studies on the interactions between these hormones may have important implications. We have investigated the effects of sodium intake, furosemide, and infusion of ANP on the urinary and metabolic (nonurinary) clearances of AVP in hydrated normal subjects. On a high sodium diet there was an increase in urine volume, sodium excretion, osmolal clearance, plasma ANP concentration, and urinary clearance and fractional excretion of AVP, with a decrease in PRA. The infusion of furosemide increased urine volume, sodium excretion, osmolal clearance, and PRA, but …

A Comparison Of An Acoustic Stethoscope And An Amplified Stethoscope In White Noise And Cafeteria Noise During Cardiac Auscultation, Lynda Lynell Gigstad

Dissertations and Theses

A basic relationship between stethoscopic auscultation and background noise interference was reviewed and examined in this study. The principle experimental design of the study questioned whether hospital background noise levels are capable of masking the threshold of detection for auscultated heart sounds. Several cited studies monitoring background noise levels in various hospital locations have reported averages exceeding the U.S. Environmental Protection Agency (EPA) (1974) and World Health Organization (WHO) (1980) recommendations of "quiet", namely 35 to 40 dBA (Falk & Woods, 1973; Hilton, 1985, 1987; Shapiro & Berland, 1972; Turner, et al., 1975; Woods & Falk, 1974) by as much …

A Physiological And Behavioral Study Of Emotional Stress In The Rat Medial Frontal Cortex, Robert J. Frysztak

Master's Theses

No abstract provided.

The Analysis Of 3-Channel "Vector" Visual Evoked Potentials And Possible Neuroanatomical Correlates, Lance Mitchell Siegel

Theses and Dissertations

he use of evoked potentials to evaluate the functional integrity of neuronal pathways has become a useful and accepted practice in clinical medicine. The use of visual evoked potentials for assisting in the diagnosis of such diseases as optic neuritis and multiple sclerosis is also well recognized. However, the visual evoked potential, unlike the auditory evoked potentials, lacks a well defined electrophysiological to neuroanatomical correlation; especially that of a significant non-cortical component. Currently, visual evoked potentials rely principally on the measurement of a cortical (occipital) positive peak of activity at 100ms (P100), recorded with a single channel electrode system, to …

Regional Differences In Connexin32 And Connexin43 Messenger Rnas In Rat Brain, C. Naus, Daniel Belliveau, J. Bechberger

Daniel J. Belliveau

The regional distribution of gap junction mRNAs was examined in the adult rat brain. The level of connexin43 mRNA is more abundant than connexin32, being homogeneously distributed throughout different regions of brain. In contrast, there is dramatic heterogeneity in the level of connexin32 mRNA, with the highest level in the hindbrain. These results suggest that the gap junction genes are differentially expressed in regions of the adult rat brain.