Research Methods in Life Sciences | Open Access Articles

Mytrials: Clinical Trial Application Concept, Katie Hollister

Graphic Communication

A clinical trial’s success is dependent upon whether a patient attends their highly-structured clinical trial visit schedule, which may include both in-person clinic visits and virtual check-in visits. MyTrials is a clinical trial journey app prototype that enables patients to schedule, reschedule, and attend join their in-person or virtual clinic visits. This product seeks to reduce the burden that scheduling places on the patient, and provides trial-provided resources and a daily and weekly timeline to help patients navigate their clinical trial journey.

Go to article

Ephemeroptera Culturing Methods: An Analysis On Rearing And Toxcity Testing On Sensitive Early Life Stages Of Native Mayfly Taxa, Geneve Rainette Edwards

Theses, Dissertations and Capstones

Standard toxicity testing organisms are utilized for regulatory purposes and often ecological risk assessments. Ephemeroptera taxa have been reported to be more sensitive to aquatic contaminants than the standard toxicity testing organisms currently used in determining effects on aquatic ecosystems. Establishing methods for culturing native Ephemeroptera taxa will provide a more sensitive test organism to determine the toxicity of contaminants and will be more representative of the responses of native taxa. Additionally, it will provide a test organism at the most sensitive life stages. The objective of this research is to develop methods for culturing and testing of native Ephemeroptera …

Go to article

Ephemeroptera Culturing Methods: Development Of Culturing And Rearing Methodology And High Sulfate Simulated Mine Effluent Toxicity Test Using Field Collected Water, Daniel Edward Brady

Theses, Dissertations and Capstones

Ephemeroptera taxa are not frequently used in toxicity testing; however, some mayfly taxa may be more sensitive to aquatic pollutants than standard test organisms used to determine anthropogenic effects on aquatic ecosystems. Additionally, some standard test organisms are not native to the Appalachian region and may not be truly reflective of the effects on native organisms. With mayflies not being the typical test organism, there is not a great deal of literature on culturing methods for this organism. For a standard acute toxicity test, there must be 80% survival within the control organisms for the test to be considered viable. …

Go to article

Conditioned Territory Defense In Madagascar Hissing Cockroaches (Gromphadorhina Portentosa), Macy R. Albaitis

Honors Theses

In male blue gourami fish (Trichogaster trichopterus), signaled presentation of a male rival produces an aggressive conditioned response that results in an increased likelihood of the male winning the contest (Hollis, 1984). I replicated this classic study in a species that also shows territorial aggression: the Madagascar hissing cockroach (MHC; Gromphadorhina portentosa). During training, four adult male MHCs were designated as “CS+” animals and received a light (NS) followed by visual access to a rival male (US). Simultaneously, another group of four adult male MHCs designated as “UNC” animals received the light (NS) and visual access to a …

Go to article

Increasing Detection Sensitivity For Rare And Endangered Species In Kansas Through Development Of An Aquatic Environmental Dna Sampling Protocol, Sarah Hallyburton

Master's Theses

Anthropogenic global climate change is forcing the mass extinction of wildlife across the globe. Humans depend on freshwater ecosystems for many aspects of life including agricultural production, sanitation, and recreation. Healthy levels of biodiversity in freshwater environments ensure economically beneficial ecosystem services are maintained. Freshwater ecosystems are hotspots for biodiversity and have higher levels of imperiled organisms compared to terrestrial and marine systems. Within freshwater systems, freshwater mussels (Family: Unionidae) are the most imperiled with over 70% of North American species assigned to a conservation class as judged by state or federal management agencies. The objective of this study was …

Go to article

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

This Senior Thesis project aimed to be able to screen samples that came in from a PhD student in Georgia for the presence of two oyster diseases, MSX and Dermo. This study was done using PCR followed by visualizing on E-gels for initial presence of disease. These were then followed by qPCR and visualization on Criterion gels. These Criterion gels were able to be analyzed in order to calculate the pg/uL of MSX and Dermo in the original ocean samples. Since the samples were sent in over the course of February-August ‘21 the presence over time was also able to …

Go to article

The Effect Of Visitor Density And Interaction On The Behavior Of Four Ray Species (Hypanus Sabina, Hypanus Say, Pseudobatos Lentiginosus, And Rhinoptera Bonasus) Housed In An Aquatic Touch Pool, Aimee Marie Little

UNF Graduate Theses and Dissertations

Human-Animal Interactions (HAI) in zoological institutions are thought to be important in helping visitors to establish a connection with animals and thus making them more likely to contribute to conservation efforts. However, animals can respond to visitor interaction in both negative and positive ways. The growing focus on animal welfare in zoological institutions emphasizes the need for assessing different environmental inputs, including visitor interaction, and how these inputs influence behavioral outputs associated with welfare. A touch pool exhibit presents a novel interactive experience that allows visitors to directly interact with various aquatic species, including elasmobranchs, whose conservation has important implications …

Go to article

Development Of Novel Pyridazine Derivatives And Drug Delivery Systems Against Dengue, Janae A. Culmer

Electronic Theses and Dissertations

The lack of approved vaccines, medications and treatment regimens has significantly contributed to the rapid spread of mosquito-borne viruses such as Dengue and Zika virus. The complex immunopathology of these viruses presents limitations for the development and implementation of a definitive, safe and effective approach to combat infections.Previous research has demonstrated that vector control strategies such as the elimination of larval habitats, larviciding with insecticides, the use of biological agents and the application of adulticides have been unsuccessful in the reduction of viral transmission leading to the need for the continued development of antivirals. This research proposes an approach for …

Go to article

Evaluating Relative Abundance, Fish Length, And Marine Protected Area Effectiveness For Four Key Rocky Reef Species Along The Northern Californian Coast, Leon Davis

Cal Poly Humboldt theses and projects

Marine protected areas (MPAs) were created with the purpose of helping conserve and restore diminished populations of marine organisms. Measuring the effectiveness of MPAs requires long-term monitoring, investigating the abundance and size distributions of the species that utilize the conservation areas, and comparing the results to neighboring reference sites that are not currently protected. In this study, observations from long-term MPA monitoring in northern California (2010-2019) were modeled with substrate, oceanographic, spatial, temporal, and body size variables to describe the variability in abundance and size of three fish groups: Black rockfish (Sebastes melanops), the Blue rockfish group (comprised …

Go to article

A Comparison Of Chimpanzee Wound Rates Before And During Covid-19 Zoo Closures, Robin Elana Salak

All Master's Theses

Visitor effect studies have had inconsistent impacts on animals, due in part to the inability to control for all confounding variables such as time of day, seasonal weather patterns, and so forth. This study represents the first instance where chimpanzee (Pan troglodytes) wounding frequencies were investigated across extensive time periods in the presence and complete absence of visitors, thus eliminating many visitor-related variables. Additional variables were eliminated through the zoo selection process, based on institutional responses to a 29-question survey, providing a novel approach to the question of visitor effects. The aim of this study was to determine …

Go to article

Investigating The Genetic Diversity Of Immune Genes In Non-Native Populations Of American Bullfrogs (Rana Catesbeiana), Luisa Nereyda Segovia

Cal Poly Humboldt theses and projects

The American Bullfrog (Rana catesbeiana) is a highly invasive species that has successfully colonized habitats around the world. The genetic variability of immune genes within invasive populations, like that of bullfrogs, may contribute to how resistant a population is to pathogens. The objective of this project was to characterize the genetic diversity of an immune gene in invasive bullfrog populations in California to better understand how persistent these populations might be over time. To characterize immune gene variability, I isolated exon 2 of the Major Histocompatibility Complex (MHC) Class II Beta chain gene and a neutral mitochondrial marker, …

Go to article

The Effects Of D2 Receptor Modulation On Locomotor Development In Danio Rerio, Isaiah Thomas Mcroberts

Senior Projects Spring 2022

This study utilized a novel design to investigate the sensitivity of D2 dopamine receptors to modulating compounds through multiple exposures over early development of zebrafish larvae. Zebrafish were dosed for 30 minutes from 5-8 days post fertilization (dpf) with 16µ/mol of either a D2 antagonist, haloperidol, or a D2 agonist, quinpirole hydrochloride. Two other groups were then dosed with these compounds from 9-12dpf. The effects of D2 receptor modulation were measured by analyzing motor activity on measures of movement distance, frequency, and velocity. Results indicated that larvae dosed with haloperidol on 5dpf had increased activity after the first dosage, but …

Go to article

Modernizing Fish Surveys: Evaluating How Reliable Environmental Dna Sampling Is In Monitoring Fish Populations, Alicia Cozza

EWU Masters Thesis Collection

No abstract provided.

Go to article

Research Methods in Life Sciences Commons^™

Full-Text Articles in Research Methods in Life Sciences

Mytrials: Clinical Trial Application Concept, Katie Hollister

Graphic Communication

Ephemeroptera Culturing Methods: An Analysis On Rearing And Toxcity Testing On Sensitive Early Life Stages Of Native Mayfly Taxa, Geneve Rainette Edwards

Theses, Dissertations and Capstones

Ephemeroptera Culturing Methods: Development Of Culturing And Rearing Methodology And High Sulfate Simulated Mine Effluent Toxicity Test Using Field Collected Water, Daniel Edward Brady

Theses, Dissertations and Capstones

Conditioned Territory Defense In Madagascar Hissing Cockroaches (Gromphadorhina Portentosa), Macy R. Albaitis

Honors Theses

Increasing Detection Sensitivity For Rare And Endangered Species In Kansas Through Development Of An Aquatic Environmental Dna Sampling Protocol, Sarah Hallyburton

Master's Theses

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

The Effect Of Visitor Density And Interaction On The Behavior Of Four Ray Species (Hypanus Sabina, Hypanus Say, Pseudobatos Lentiginosus, And Rhinoptera Bonasus) Housed In An Aquatic Touch Pool, Aimee Marie Little

UNF Graduate Theses and Dissertations

Development Of Novel Pyridazine Derivatives And Drug Delivery Systems Against Dengue, Janae A. Culmer

Electronic Theses and Dissertations

Evaluating Relative Abundance, Fish Length, And Marine Protected Area Effectiveness For Four Key Rocky Reef Species Along The Northern Californian Coast, Leon Davis

Cal Poly Humboldt theses and projects

A Comparison Of Chimpanzee Wound Rates Before And During Covid-19 Zoo Closures, Robin Elana Salak

All Master's Theses

Investigating The Genetic Diversity Of Immune Genes In Non-Native Populations Of American Bullfrogs (Rana Catesbeiana), Luisa Nereyda Segovia

Cal Poly Humboldt theses and projects

The Effects Of D2 Receptor Modulation On Locomotor Development In Danio Rerio, Isaiah Thomas Mcroberts

Senior Projects Spring 2022

Modernizing Fish Surveys: Evaluating How Reliable Environmental Dna Sampling Is In Monitoring Fish Populations, Alicia Cozza

EWU Masters Thesis Collection