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The Germfree Culture Of Three Species Of Triatominae: Triatoma Protracta (Uhler), Triatoma Rubida (Uhler), And Rhodnius Prolixus Stål, Stephen A. Nyirady
The Germfree Culture Of Three Species Of Triatominae: Triatoma Protracta (Uhler), Triatoma Rubida (Uhler), And Rhodnius Prolixus Stål, Stephen A. Nyirady
Loma Linda University Electronic Theses, Dissertations & Projects
Triatoma protracta protracta, Triatoma riibida rubIda, and two strains of Rhodnius prolixus were successfully reared germfree into the fourth generation. Triatominae eggs were disinfected externally with iodine and the nymphs reared in vinyl plastic germfree modules. Germfree mice were used as the host. This is the first report of Triatominae having been reared in a germfree environment. These bugs were never exposed to bacteria internally or externally to the fourth generation.
Comparisons of conventional control bugs with replicate germfree groups in the first two generations revealed but slight differences in developmental periods. The mean adult longevity of all …