Research Methods in Life Sciences Commons^™

Effects Of Dietary Factors And Morphine Administration On Voluntary Alcohol Consumption And Dopamine Metabolism In Rats, Claudia Smithson

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of diet and dietary additives on voluntary alcohol consumption in male Sprague-Dawley rats was studied. Alcohol intake measured weekly and reported as ml of a 10% solution ingested per 100 grams body weight. A four week pre-experimental period was designed to determine individual tendencies to drink and thus to eliminate the high and low drinkers from the study. No difference was found in alcohol intake with either starch or sugar as the carbohydrate source; however, there was a greater weight gain ( p < .025) in those animals consuming sugar-based diets. Rats on poor-quality diets containing minimal amounts of vitamins and minerals continued to gradually increase alcohol consumption during the study. This diet, supplemented with coffee, caused the greatest significant increase in alcohol intake and the lowest weight gains. Addition of coffee to the diets significantly increased alcohol consumption (p< .005) but decreased growth rate (p < .0005) . However, when the poor quality diets were supplemented with vitamins and minerals, alcohol consumption was reduced by about 50% (p < .005) and weight gain was significantly increased (p < .0005).

The daily intraperitoneal administration of morphine sulfate significantly decreased alcohol intake (p < .02) and the per cent of total fluid consumed as alcohol (p < .02) but did not effect water consumption.

An attempt was …

The Germfree Culture Of Three Species Of Triatominae: Triatoma Protracta (Uhler), Triatoma Rubida (Uhler), And Rhodnius Prolixus Stål, Stephen A. Nyirady

Loma Linda University Electronic Theses, Dissertations & Projects

Triatoma protracta protracta, Triatoma riibida rubIda, and two strains of Rhodnius prolixus were successfully reared germfree into the fourth generation. Triatominae eggs were disinfected externally with iodine and the nymphs reared in vinyl plastic germfree modules. Germfree mice were used as the host. This is the first report of Triatominae having been reared in a germfree environment. These bugs were never exposed to bacteria internally or externally to the fourth generation.

Comparisons of conventional control bugs with replicate germfree groups in the first two generations revealed but slight differences in developmental periods. The mean adult longevity of all …

Marrow Isografts In The Oral Cavity Of Mice, Robert L. Merin

Loma Linda University Electronic Theses, Dissertations & Projects

The objectives of this study were to develop an animal model for testing oral bone and marrow transplantation that is adaptable to immunological studies; to determine the effect of an isogeneic graft of cancellous bone and marrow on the healing of surgically created defects; and to determine the fate of grafted isogeneic cancellous bone and marrow transplanted into defects. Fourteen CBA/H mice served as experimental animals and bilateral intraoral defects were prepared. The experimental side received isogeneic graft material while the other side served as a control. Osseous healing was found to be accelerated in the defects receiving graft material …

Microscopic Evaluation Of Topically Applied Chlorhexidine Gluconate On Gingival Wound Healing In Dogs, Robert C. Hirst

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate microscopically the effect of topically applied 2% chlorhexidine gluconate on the healing of gingival wounds in dogs.

One week after the teeth were scaled and polished, seven experimental wound sites were prepared in the facial gingiva adjacent to the four premolars on each side of the maxillary arch in six young Labrador dogs. From this point the dogs were fed a soft plaque-inducing diet. Chlorhexidine solution was applied daily to either the right or left maxillary premolars while on the opposite side of the arch in three dogs the first and second …

The Effect Of Oral Allografts Of Cancellous Bone And Marrow In Mice, George E. Deeb

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was: to develop a model for testing oral bone transplantation; to study the healing of a surgically produced defect with and without the transplantation of strongly allogeneic cancellous bones and marrow; and to determine the fate of the allogenic grafts. Twenty-eight CBA/H mice served as experimental animals. Intra-oral defects were made and in 14 of the animals strongly allogenic cancellous bone and marrow were transplanted. The 14 remaining animals served as controls. It was observed that the healing was delayed in the experimental animals as compared to the controls and that the grafted material was …