Research Methods in Life Sciences Commons^™

Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker

Graduate Theses and Dissertations

The need to study and monitor amphibian populations is increasing along with the threats to their population stability and persistence in nature. Northwest Arkansas is one of the fastest growing areas in the nation and with that growth comes rapid changes in land use, massive alterations to habitats, habitat loss, and the introduction of nonnative plants and animals. Ambystoma annulatum (Ringed Salamander) is an Ambystomatid endemic to the Ozark and Ouachita Mountains of southern Missouri, northern and western Arkansas, and eastern Oklahoma giving it a relatively small distribution compared to most Ambystoma. Therefore, Arkansas constitutes a significant portion of the …

In Vitro And In Vivo Evaluation Of Antimicrobial Activity, Oocyst Sporulation Rate, Oocyst Integrity And Oocyst Infectivity In Eimeria Maxima M6 Oocyst Suspensions Treated With Chlorhexidine Salts In Comparison With Potassium Dichromate, Lauren Laverty

Graduate Theses and Dissertations

Eimeria species oocysts cause coccidiosis, one of the costliest diseases in the poultry industry. Coccidiosis infections cause significant economic losses due to reduced performance in chickens and turkeys. The objective of this thesis was to determine fecal bacterial recovery from chlorhexidine (CHX) salt suspensions containing Eimeria maxima M6 oocysts and evaluate the effect of CHX salts on oocysts during long-term cold storage. For bacterial recovery experiments, CHX digluconate and CHX gluconate were tested at different concentrations on poultry fecal slurries during oocyst shedding. Eimeria maxima M6 oocysts were evaluated in CHX salt suspensions to test the effect on oocyst degradation …

Assessment Of Dietary Energy And Amino Acid Diet Formulation In The Modern Broilers, Matheus Freitas Costa

Graduate Theses and Dissertations

As a result of the advancement of genetics, understanding broiler requirements for dietary energy and amino acids is essential for improving live performance and carcass characteristics. The energy system used to formulate diets in poultry is based on metabolizable energy (ME); this equation can be described as the total amount of energy in the feed minus gases and feces. However, the net energy (NE) system may be the best depiction of the energy utilized by birds for production and maintenance purposes once the heat increment (HI) is measured. Thus, a series of studies were conducted to establish the responsiveness of …

Factors That Affect Home Range Of Timber Rattlesnakes (Crotalus Horridus) In Northwest Arkansas, Bannon Gallaher

Graduate Theses and Dissertations

Conservation of animal populations requires knowledge of their habitat and spatial needs. Quantifying spatial requirements involves the analysis of home range. We examined the effects of sex, body size (SVL), body condition (log mass/log SVL), and year on home range in Timber Rattlesnakes (Crotalus horridus) in Northwest Arkansas. Individual locality data from an ongoing, 22+ year radio-telemetry study in Madison Co., Arkansas were analyzed using both minimum convex polygon (MCP) and Kernel Density Estimates (KDE). Plots of the number of sequential observations versus home range (MCP and KDE) determined that a minimum of 25 locations per individual per active season …

The Effect Of Spirulina Platensis Algae Inclusion In Feed Of Commercial Broilers Subjected To Cyclic Heat Stress, Kirsten Shafer

Graduate Theses and Dissertations

The effect of Spirulina platensis inclusion in feed of commercial broilers was evaluated over one experiment. The study aimed to investigate the effects of Spirulina platensis freshwater algae inclusion on live performance and processing characteristics of commercial broilers subjected to daily cyclic heat stress. Day old Ross 708 male broilers were placed into 8 environmentally controlled chambers. At d 21, four chambers remained on a control diet containing no algae (CON) while 4 chambers received a diet that included algae at an inclusion rate of 2.5% (ALG). An 8 hour daily cyclic heat stress (24°C to 36°C) was applied to …

Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta

Graduate Theses and Dissertations

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …

Identification Of Possible Vectors For Histomoniasis In Turkeys On Commercial Farms In Arkansas And Oklahoma, Treva Dillon

Graduate Theses and Dissertations

Histomonas meleagridis, is a parasitic protozoan, commonly found in the gallinaceous species of birds and is the causative agent of Blackhead disease. The cecal worm, known as Heteraskis gallinarum, acts as a vector in the spread of H. meleagridis in gallinaceous birds. H. meleagridis develops inside the cecal worm and in its eggs which allows H. meleagridis a method to survive in the environment. Blackhead disease causes high mortality rates in both chickens and turkeys; however, turkeys are noted to have a higher mortality rate compared to chickens. Molecular diagnostic testing such as PCR has been developed by creating primers …

Optimization Of Clostridium Septicum Antigen Production And Evaluation Of Vaccine Administration Parameters For A Candidate Bacterin-Toxoid To Prevent Dermatitis In Commercial Turkeys, Aaron Forga

Graduate Theses and Dissertations

The objective of this thesis was to optimize the hemolytic activity of two isolates of Clostridium septicum to evaluate if hemolytic titer of C. septicum antigen at time of formalin inactivation corresponded to increased serum antibody titer to the C. septicum alpha-toxin of turkeys immunized with an experimental formalin inactivated bacterin-toxoid vaccine. Chapter 1 provides a brief overview of the thesis. Chapter 2 consists of a literature review focused on clostridial dermatitis, specifically C. septicum-associated dermatitis (cellulitis) that affects commercial turkeys. Chapter 3 outlines our attempts to optimize an experimental autogenous bacterin-toxoid vaccine as a potential strategy to mitigate clostridial …

Effects Of An Injectable Zinc Solution At Weaning As An Alternative Castration Method In Beef Cattle, Reagan Nicole Cauble

Graduate Theses and Dissertations

Two experiments were conducted to investigate a 1 mL intratesticular zinc (100 mg Zn) injection administered into each testicle at weaning (Z) and its effects on growth, health, behavior and testosterone. In the first experiment, beef bulls were assigned randomly to treatment at birth: 1) surgically castrated at birth (S; n = 37) or 2) Z (n = 37). Testicular thickness differed by day (P < 0.01) for Z. Testosterone concentrations were greater in Z by d 77 and remained so through d 280 (P = 0.02) compared to S. Zinc injected calves were heavier at the trail’s conclusion (P ≤ 0.04), had greater ADG overall (P < 0.01), heavier hot carcass weight (P = 0.01), and greater lean muscle area (P = 0.01) compared to S; but a lower marbling score (P < 0.01). Yield Grade, dressed carcass yield and fat thickness were similar (P ≥ 0.14). Zinc injected calves had greater haptoglobin (Hp) concentrations (P < 0.01). Zinc injected calves had greater concentrations of white blood cells on d 1 and 2 and greater concentrations and proportions of neutrophils on d 1, 2 and 3 (P < 0.01). Conversely, S had a greater percentage of lymphocytes on d 1, 2 and 3 and a lower ratio of neutrophils to lymphocytes during that same time (P < 0.01). Zinc injected calves spent more time on their side or sternum on d 1 and more time on their side on d 2 while S spend more time standing during that time (P < 0.01). During d 3, 4, 5, and 6, Z stood more while S laid on their sternum (P < 0.01). In trail 2, beef bulls were allocated randomly to treatment one week post weaning: 1) banded (B; n = 42) or 2) Z (n = 39). Body weights were similar (P ≥ 0.39) but ADG improved for Z compared to B (P = 0.05). Testosterone concentrations were greater in Z compared to B (P ≤ 0.02). Testicular width in Z differed by d (P < 0.01). Zinc castrated calves spent more time on their side compared to B one day post castration (P = 0.03) until d 3 and 4 where B spent more time on their side (P ≤ 0.02). Banded calves stood more the first two days (P ≤ 0.01). On days 3, 4, 5, and 6, Z stood more (P ≤ 0.01) while B were on their sternum (P ≤ 0.03). Banded calves had lower Hp concentrations compared to Z (P ≤ 0.01). Total white blood cell concentrations, proportions and concentrations of neutrophils, and proportion of lymphocytes were greater in Z compared to B on d 1, 2, and 3 (P ≤ 0.01). There was no interaction between treatments over time for IL1β, IL6, and TNFα expression (P ≥ 0.83). The findings in both studies indicate that intratesticular Zn injections at weaning can improve growth performance and some carcass attributes but produce a heightened inflammatory and immune response and cause discomfort. Injecting zinc, as formulated and administered in these trails, does not result in complete castration of beef calves at this age and therefore cannot be considered a true castration alternative.

Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole Mckinney

Graduate Theses and Dissertations

Poultry is a staple protein source for most of the planet. Until recently, antibiotic growth promoters (AGPs) were used to prevent illnesses in commercial chicken production. Currently, this is not possible due to regulations and consumer concern, but without such a preventative, diseases like necrotic enteritis (NE) have reemerged, posing a threat to bird health, and ultimately, our food source. Necrotic enteritis is a severe gastrointestinal disease caused by the gram-positive pathogen, Clostridium perfringens. Clinical features of this disease are diarrhea, intestinal lesions, and death, with a high transmission rate. In a subclinical form, growth performance is diminished and is …

Effect Of Sensory Additives On Sow Lactation Performance, Subsequent Reproductive Performance, And Nursey Pig Performance, Nathan E. Davis

Graduate Theses and Dissertations

Maximizing nutrient intake during lactation is vital to sow performance as well as litter performance. The aim of these studies was to evaluate the impact of a sensory additive on sow lactation performance and nursery pig performance. Five groups of sows were farrowed, three during winter (October, December and January) and two during summer months (June, July, August). Sows were weighed, and back fat depth was measured, at 110 d of gestation prior to entering the lactation room, and again at weaning. The sows were blocked by parity, and then allotted by BW at d 110 to Control or 0.075% …

The Role Of Microbiota On Campylobacter Jejuni Colonization And Growth Performance In Broiler Chickens, Ayidh Almansour

Graduate Theses and Dissertations

The successful poultry production at modern era comes from the vertical integratedindustrialization, which has fundamentally changed how animals have been living for millions of years. Antimicrobial growth promoters have been used to sustain the efficient industrialized animal production, driving antimicrobial overuse and resistance. Because of the increasing pressure from consumer’s concerns and government regulations, it is urgent to develop antimicrobial free alternatives as growth promoters in poultry production, but few effective antimicrobial alternatives are currently available. Campylobacter jejuni is one of the worldwide prevalent foodborne bacterial pathogens mainly transmitted from poultry. However, few mechanisms are available on why C. jejuni …

Investigating Molecular Mechanisms Behind Bacterial Chondronecrosis With Osteomyelitis (Bco) Pathogenesis In Modern Broilers, Alison Ramser

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis (BCO), a leading cause of lameness in broiler chickens, is characterized by infection, inflammation, and bone attrition. There are currently no effective treatments and positive diagnosis is only possible through necropsy evaluations. Lameness is also a rising animal welfare and economic concern, making prevention and detection of BCO all the more critical. These challenges are exacerbated by a lack of mechanistic understanding of BCO’s etiology. The question I asked during my dissertation was how bacteria induce bone attrition in BCO pathology. My research has shown that mitochondrial dysfunction is characteristic of BCO conditions along with autophagy …

Pandemics And Animal Welfare: A Quantitative Inquiry Into How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Kayla N. Desmet

Animal Science Undergraduate Honors Theses

Since the emergence of the COVID-19 pandemic in late 2019, industries have been impacted globally. The companion animal care industry has not received much recognition in mainstream news, and though multiple grants have been allocated (Nolen, 2020), limited resources were available to individual facilities such as animal shelters, animal day cares, and boarding facilities. Little is known about the long-term effects of the pandemic on these facilities and how companion animal care may have changed. The purpose of this study was to explore the day-to-day impacts and long-term effects of COVID-19 on the companion animal industry in Northwest Arkansas from …

Pets And Problems: Exploring Community Patterns In Calls For Animal Services, Paige Dejarnett

Sociology and Criminology Undergraduate Honors Theses

Pets have historically been viewed as family members, children, property, or economic resources. However, research surrounding animal maltreatment has expressed this issue as an individually-based problem, rather than a community-based phenomenon. Correlations have been found between animal cruelty, antisocial behaviors, and future interpersonal violence, whether this correlation be a predictive relationship, or a resultant relationship. Past research has also found correlations between animal treatment practices and the rural/urban differences of this behavior. However, there are many community-based indicators that have not been explored to understand the distribution of animal maltreatment. This study aims to explore these ideas by analyzing the …

Studies Of Avian Nasal Mites (Acari: Rhinonyssidae And Ereynetidae) And Their Interaction With The Brood Parasite Brown-Headed Cowbird (Molothrus Ater: Icteridae) And Phylogenetic Inferences Of The Genus Ptilonyssus (Rhinonyssidae) On Different Passerine Hosts Associated To Three States In The Us, Alexis D. Hilario Perez

Graduate Theses and Dissertations

Nasal mites are endoparasites that spend their entire life cycle inside the nasal cavities and respiratory passages of birds. The Brown-Headed cowbird (Molothrus ater) (BHCO) is an icterid bird that uses brood parasitism as a reproductive strategy in which it lays an egg in the nest of a different bird species and allows the host to raise its young. Interestingly, nasal mites reported from cowbirds represent the same species found infesting other icterids and other common host groups. In the first study, I examined how diversity and host prevalence might change in a large sample size of BHCO associated with …

Investigation Of Histomoniasis Prevention In Poultry, Lesleigh Beer

Graduate Theses and Dissertations

Histomonas meleagridis is the etiological agent of histomoniasis, also commonly known as blackhead disease. This protozoal disease of poultry is detrimental to turkeys with flock mortalities often reaching 80-100%, although other gallinaceous birds are susceptible. Since the voluntary removal of nitarsone in 2015, the poultry industry is suffering with no approved prophylactics, therapeutics, or vaccines for this disease. The objectives of this dissertation were to evaluate multiple methods for prevention or control of histomoniasis, including dietary chemoprophylaxis and vaccination. Specifically, this research evaluated quinine as a chemoprophylactic candidate (Chapter 3) or live-attenuated H. meleagridis as vaccine candidates (Chapter 4) in …

Reducing Dietary Crude Protein: Characterization Of Branched-Chain Amino Acid Antagonism In Modern Broiler Chickens, Craig William Maynard

Graduate Theses and Dissertations

Reducing the crude protein content of broiler diets while maintaining performance has been a primary research area in nutrition. During the late 20th century, the bulk of this research has involved supplementing broiler diets with feed-grade amino acids and proving the economic benefits of their additions. To date, widespread adoption of feed-grade amino acids is limited to methionine, lysine, threonine, and to some extent valine. With valine and isoleucine gaining prevalence in commercial formulation, classic data on the antagonisms associated with the branched-chain amino acids has returned to the forefront of practical broiler nutrition research. Therefore, a series of 23 …

Directed Genome Evolution To Identify Genes For Macrophage Survival By Staphylococcus Agnetis, Sonali Lakshika Anne Lenaduwe Lokuge

Graduate Theses and Dissertations

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a debilitating infection that negatively impacts animal welfare and costs the broiler industry billions of dollars annually. We have previously isolated Staphylococcus agnetis 908 from BCO samples obtained from broilers at the University of Arkansas research farm. This isolate can induce BCO lameness at greater than 50% in broilers exposed to the pathogen in drinking water. We found that S. agnetis 908 is capable of surviving and escaping macrophages compared to a closely related cattle isolate,1379. Through Directed Genome Evolution (DGE) we identified that this difference is at least partially associated with an alanine …

Influence Of Local- And Landscape-Scale Factors On Avian Assemblage In Fragmented Tallgrass Prairie Landscape, Pooja Panwar

Graduate Theses and Dissertations

Agricultural expansion and increasing urbanization are driving rapid landscape modification in the Tallgrass prairies ecosystem and are affecting biodiversity at multiple spatial scales. Thus, the conservation of fragmented grassland and steeply declining grassland avifauna is dependent on the relationship between local- and landscape-scale factors and avian assemblage. We also need to understand how these factors, operating at different spatial scales, drive the occupancy of different guilds in the assemblage. We conducted repeated point count surveys during the breeding season of 2019 at 66 grassland sites across Arkansas and Missouri, USA. We developed a multi-species occupancy model to estimate species richness, …

Evaluation Of Nutritional Factors That Influence The Efficacy Of Tributyrin As A Feed Additive For Broilers, Brooke Bodle

Graduate Theses and Dissertations

Tributyrin (TB) is a glyceride ester of butyrate that has the potential to improve broiler performance and intestinal development. Therefore, to fully evaluate this potential, three experiments were conducted to evaluate effect of tributyrin (TB) on broiler growth performance, nutrient digestibility, carcass characteristics, intestinal morphology, and gastrointestinal function in birds fed diets varying in composition reared in battery cages and floor pens. Experiment 1 explored the supplementation of graded doses of TB and its impact upon growth performance, nutrient digestibility, and carcass characteristics in a step-down program when added to a reduced energy and amino acid diet. A linear reduction …

Relationship Of Underline Scores And Production Traits In Beefmaster Cattle, Bryan R. Kutz

Graduate Theses and Dissertations

The objective of this study was to establish the relationship of underline scores (UDLS), production traits, and conformation scores in Beefmaster cattle born between 1976 through 2008. Records provided by Beefmaster Breeders United (BBU) were analyzed. Certified classifiers approved by the BBU recorded scores. Sizes and shapes of the underlines differentiated the scores (1-4). A score of one was used to designate the least amount of navel flap or sheath and a score of four represents the maximum amount of naval flap or sheath accepted for registration in the BBU. In this study the relationship of the traits was determined …

Climate-Driven Impacts On Himalayan Aquatic Biodiversity: A Case Study Involving Snowtrout (Cyprinidae: Schizothorax), Riri Wiyanti Retnaningtyas

Graduate Theses and Dissertations

Monitoring biodiversity, to include its relative dispersal and contraction, has become a conservation task of great importance, particularly given the catastrophic and ongoing loss of habitat due to climate change. However, the timing, direction, and magnitude of these rates vary across taxa and ecosystems. Predicting specific impacts of climate change can thus be difficult and this, in turn, hampers management action. Metrics are needed to not only quantify contemporary requirements of species, but also predict potential distributions that fluctuate in lockstep with climate.

Montane ecosystems in the Himalayas are highly impacted by climate change, yet remain largely understudied due to …

Evaluation Of Weight Gain, Feed Intake, Feed Conversion And Oocyst Shedding Of Eimeria Maxima And Eimeria Acervulina In Broiler Chickens, Lauren Laverty

Poultry Science Undergraduate Honors Theses

The purpose of the present study was to evaluate the day and the time of sample collection of an

experimental challenge with Eimeria maxima (EM) and Eimeria acervulina (EA) in broiler chickens. One day old male Cobb-Vantress broiler chickens were randomly allocated to one of three groups with ten replicates (n=8 chickens/replicate). Chickens were placed in battery cages with a controlled age-appropriate environment: Group 1) Negative control (no challenge or treatment); 2) Challenge control (Eimeria challenge only); 3) Challenge + Salinomycin. Challenged chickens were orally gavaged with the mixed culture of EM/EA (10,000 sporulated EM containing 4% wild-type EA) …

Role Of Reptile-Invertebrate Interactions In Enigmatic Reptile Declines, Meredith Swartwout

Graduate Theses and Dissertations

Reptile populations are declining world-wide and the mechanisms behind many of these declines remain enigmatic. Food web interactions (i.e., reduced prey availability or increased predation) have been implicated behind some reptile declines. However, relatively little is known about predation on lizard and snake eggs, despite egg survival being important for population dynamics of some species. Ants are important predators of squamate reptile eggs in tropical and temperate systems. In Costa Rica, long-term declines in terrestrial anole lizards were linked with reduced leaf litter depth, a factor that could influence egg vulnerability to ant predation. Fire ants (genus Solenopsis) are aggressive …

Survey Of Ticks And Tick-Borne Pathogens Associated With Feral Swine (Sus Scrofa) In Arkansas, Haylee Campbell

Graduate Theses and Dissertations

Feral hogs (Sus scrofa L.) are an invasive species throughout the southeast United States and found in every Arkansas county. As feral hogs invade new habitat, they can disrupt ecosystems, damage agriculture systems, and bring ticks and tick-borne pathogens with them. There are no surveys of the tick species parasitizing the Arkansas feral hog populations or the pathogens they carry. This is a public health concern because feral hogs occupy often same geographical regions as humans and livestock and can harbor over 45 animal diseases and parasites. The ticks carried by feral hogs can carry tick-borne pathogens that can cause …

Methods To Reducing Weaning Stress In Early Weaned Spring Beef Calves, Matthew Daniel Hahn

Graduate Theses and Dissertations

Reducing weaning stress in beef calves can have dramatic benefits to the health, growth, and performance to beef calves post-weaning. Studies have shown that providing creep feed to calves during the pre-weaning period can improve their nutritional status, which can better prepare them for the stresses they will be exposed to during the weaning process. Studies have also shown that two-stage weaning can reduce the stresses associated with weaning. Previous research has suggested that this method resulted in dramatic changes in behavioral signs associated with stress. The objective of this study was to evaluate the effects of two-stage weaning with …

Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis is the most important cause of lameness in broilers. This is important to poultry production, as it poses animal welfare issues, and causes a significant loss in revenue. The remediation of this disease requires the study of its etiology with fitting models and evaluating preventatives. The research reported herein covers genomic virulence analysis of BCO isolates, mainly Staphylococcus aureus, and Escherichia coli retrieved from lame birds. We found that S. aureus isolates were closest to chicken strains in Europe but may have been in the Arkansas area for a decade. Phylogenomics suggest our S. aureus is …

Effects Of Commercial Feed Additives On Growth Performance And Gut Microbiota Of Nursery Pigs, Xiaoyuan Wei

Graduate Theses and Dissertations

To secure animal welfare and maximum production, feed additives are often added to nursery pig diets to increase nutrition digestibility, enhance intestinal health, and prevent or mitigate disease, with the ultimate goal of improving growth performance. This thesis performed a longitudinal analysis using next-generation sequencing to investigate the dynamic changes of gut microbiota in weaned pigs fed commercial feed additives. Chapter II was used to evaluate the effects of organic acid mixture on growth performance and gut microbiota of weaning pigs. This study demonstrated that the inclusion of 0.035% (SBA0.035) or 0.070% (SBA0.070) sodium butyrate in a diet containing 0.5% …

Evaluation Of Selected Bacillus Direct-Fed Microbial Candidates In Reduced Energy Diets On Live Performance, Carcass Characteristics, And Foot Pad Dermatitis, Kyle Teague

Graduate Theses and Dissertations

Bacillus spp. are ubiquitous, Gram-positive, spore forming bacteria that are commonly recovered from the environment and gastrointestinal tract (GIT) of poultry. These spores are capable of withstanding harsh condition such as feed pelletization, which facilitates inclusion in poultry feeds. Once ingested by the bird, spores germinate into metabolically active vegetative cells which can produce extracellular enzymes which can hydrolyze otherwise indigestible components of the feed. Soybean meal (SBM) is the gold standard vegetable protein source for non-ruminant animals worldwide and is included in practically all poultry diets in the United States at levels ranging from 10 to more than 30%. …