Research Methods in Life Sciences Commons^™

Quantifying The Respiratory Plasticity Of Common Fishes Of The Indian River Lagoon, Logan Exton

Honors Theses

Increasing water temperatures resulting from global climate change introduce new energetic demands for marine organisms. Higher energy input will be required to cope with a subsequently higher metabolic rate, affecting all aspects of an individual’s life and therefore their survival. Because estuaries act as a link between rivers and oceans, they and their inhabitants are considered to be the most threatened by climate change. Therefore, it is crucial to understand how these organisms will respond to increased stressors due to climate change. Checkered pufferfish (Sphoeroides testudineus) are among the most common teleost fish in the Indian River Lagoon, …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

Eliciting The Effect Free Roaming Ads Have On Native Hawaiian Wildlife, Erin Salano

Theses and Dissertations--Biology

This study measured the concentration of the stable isotopes Carbon and Nitrogen within the tissues of free-roaming and indoor cats on the Hawai’ian islands. This study aimed to determine whether cats are consuming more human provisioned or wild food sources and whether their diet is having a negative impact on endemic species; with a focus on assessing impacts on conserved bird populations. We hypothesized that human provisioned food sources have higher levels of carbon due to the amount of corn fillers within them, while wild food sources are more Nitrogen rich. We found that indoor and feral cats sampled from …

Puberty-Associated Changes In Kiss1r, Mc3r, And Mc4r In The Ewe, Ashleigh Caitlyn Thomson

Graduate Theses, Dissertations, and Problem Reports

The neural mechanisms that control the onset of puberty are not completely understood. In livestock, the onset of puberty relies on an increase in gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH). Kisspeptin, neurokinin B, and dynorphin neurons (KNDy) and their receptors, Kiss1r, NK3R, and KOR, respectively, are important regulators of puberty. Previous data from our laboratory has shown that KNDy neurons may be responding to other inputs, including the melanocortin system, that will affect the timing of puberty. Given this, we examined kisspeptin receptor (Kiss1r) mRNA expression in GnRH neurons as well as examining melanocortin 3 and 4 receptor …

Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker

Graduate Theses and Dissertations

The need to study and monitor amphibian populations is increasing along with the threats to their population stability and persistence in nature. Northwest Arkansas is one of the fastest growing areas in the nation and with that growth comes rapid changes in land use, massive alterations to habitats, habitat loss, and the introduction of nonnative plants and animals. Ambystoma annulatum (Ringed Salamander) is an Ambystomatid endemic to the Ozark and Ouachita Mountains of southern Missouri, northern and western Arkansas, and eastern Oklahoma giving it a relatively small distribution compared to most Ambystoma. Therefore, Arkansas constitutes a significant portion of the …

In Vitro And In Vivo Evaluation Of Antimicrobial Activity, Oocyst Sporulation Rate, Oocyst Integrity And Oocyst Infectivity In Eimeria Maxima M6 Oocyst Suspensions Treated With Chlorhexidine Salts In Comparison With Potassium Dichromate, Lauren Laverty

Graduate Theses and Dissertations

Eimeria species oocysts cause coccidiosis, one of the costliest diseases in the poultry industry. Coccidiosis infections cause significant economic losses due to reduced performance in chickens and turkeys. The objective of this thesis was to determine fecal bacterial recovery from chlorhexidine (CHX) salt suspensions containing Eimeria maxima M6 oocysts and evaluate the effect of CHX salts on oocysts during long-term cold storage. For bacterial recovery experiments, CHX digluconate and CHX gluconate were tested at different concentrations on poultry fecal slurries during oocyst shedding. Eimeria maxima M6 oocysts were evaluated in CHX salt suspensions to test the effect on oocyst degradation …

Assessment Of Dietary Energy And Amino Acid Diet Formulation In The Modern Broilers, Matheus Freitas Costa

Graduate Theses and Dissertations

As a result of the advancement of genetics, understanding broiler requirements for dietary energy and amino acids is essential for improving live performance and carcass characteristics. The energy system used to formulate diets in poultry is based on metabolizable energy (ME); this equation can be described as the total amount of energy in the feed minus gases and feces. However, the net energy (NE) system may be the best depiction of the energy utilized by birds for production and maintenance purposes once the heat increment (HI) is measured. Thus, a series of studies were conducted to establish the responsiveness of …

Deep Ocean Vehicle Applications And Modifications, Nichole "Nikki" T. Arm

Master's Theses

This project had two primary goals: (1) to explore opportunities to further a deep-ocean vehicle’s reach using alternative pressure spheres, and (2) to implement an existing deep-ocean vehicle (lander) in active scientific research.

I gained a greater understanding of the limitations and design choices made for existing pressure spheres using Finite Element Analysis (FEA). My simplified FEA model predicted sphere failure for the existing 30% Fiber Glass 70% Nylon injection molded spheres at an external pressure of 3,954psi or 2,690m ocean-depth (only a 7.38% error compared to the tested minimum failure depth), so I determined it a valid model. I …

Evaluation Of The Virulence Potential Of Avian Pathogenic Escherichia Coli Isolated From Broiler Breeders With Colibacillosis In Mississippi, Jiddu Joseph

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is a bacterium that is responsible for colibacillosis in birds. However, information about broiler breeder APEC isolates is limited, but the data is critical due to the transfer of this bacteria down the production pyramid to progenies resulting in high mortality. Therefore, we evaluated the phenotypic virulence characteristics of 28 isolates using embryo lethality and day-old chick challenge assays. Also, the in vitro adhesion and invasion potential of selected nine isolates were identified. Results showed more than 1/3^rd of the isolates were highly virulent and the virulence increased as the number of virulence-associated genes …

An Evolutionary Comparative Study Of Congenital Stationary Night Blindness-Associated Trpm1 Genetic Variants Of Uncertain Significance In Horses And Humans Utilizing Caenorhabditis Elegans, Gabrielle Davis

Theses

Congenital stationary night blindness (CSNB) is a heterogeneous collection of genetic diseases affecting the eyes and vision in horses and humans. Current research has implicated several genetic mutations impacting different genes involved in phototransduction and signal transmission, including TRPM1. In horses, genetic mutations in TRPM1 also result in a leopard spotting pattern or leopard complex. The goal of this study is to examine the potential impact of CSNB associated TRPM1 missense variants of uncertain significance (VUS). Previous research in Caenorhabditis elegans have revealed an orthologous TRPM1 gene known as gon-2 that allows for comparative studies. The evolutionary relationship of …

Factors That Affect Home Range Of Timber Rattlesnakes (Crotalus Horridus) In Northwest Arkansas, Bannon Gallaher

Graduate Theses and Dissertations

Conservation of animal populations requires knowledge of their habitat and spatial needs. Quantifying spatial requirements involves the analysis of home range. We examined the effects of sex, body size (SVL), body condition (log mass/log SVL), and year on home range in Timber Rattlesnakes (Crotalus horridus) in Northwest Arkansas. Individual locality data from an ongoing, 22+ year radio-telemetry study in Madison Co., Arkansas were analyzed using both minimum convex polygon (MCP) and Kernel Density Estimates (KDE). Plots of the number of sequential observations versus home range (MCP and KDE) determined that a minimum of 25 locations per individual per active season …

Fecal Pellet Production By North Atlantic Zooplankton, Michael Gibson

Undergraduate Honors Theses

Fecal pellet carbon (FPC) production by zooplankton is a significant component of the ocean’s biological carbon pump: the suite of biological processes that mediate export of carbon to the deep ocean, ultimately leading to the sequestration of atmospheric carbon dioxide in the ocean. In this study, mesozooplankton (zooplankton 0.2 mm to ~2 cm) were collected from the epipelagic zone in the temperate North Atlantic Ocean during day and night in May 2021. Zooplankton were live separated into five size fractions and incubated on board ship in natural surface seawater to measure fecal pellet production rate of the mixed mesozooplankton community. …

Determining Species-Specific False-Positive Rates Using Visual And Auditory Cues: A Case Study With Sagebrush Steppe Songbirds, Amelia K. Evavold

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Errors in wildlife field data threaten to bias resulting abundance and occupancy estimates if not properly accounted for or minimized. Methods to account for false-positive errors in wildlife data have not been as thoroughly developed as those for false-negative errors despite false-positives being present across diverse wildlife taxa and study systems. The calibration method to account for false-positives involves assessing the field detection method to determine how often false-positive errors occur in the field data. Rates can then be incorporated into estimations based on the field data to improve estimation accuracy. This study presents an application of the calibration approach …

Genetic Evaluation Of The Current Distribution And Possible Diffrentations Between Lasiurus Borealis And Lasiurus Frantzii In Southwestern North America, Zeinab M. Haidar

Cal Poly Humboldt theses and projects

Several recent accounts of overlap and historic misidentifications regarding two species of the genus Lasiurus, Western red bat (Lasiurus frantzii) and Eastern red bat (Lasiurus borealis), have cast doubt on our understanding of their distribution, assumed spatial allopatry, and interactions in the United States Southwest. With the use of morphometrics and genetic sequencing, utilizing tissue collected from specimens throughout California and adjoining states, we have reassessed the current distribution, best practices for field identification, and genetic differentiation between both species. Appropriate species classification by region was achieved utilizing mitochondrial DNA, targeting the cytochrome c oxidase …

The Effect Of Spirulina Platensis Algae Inclusion In Feed Of Commercial Broilers Subjected To Cyclic Heat Stress, Kirsten Shafer

Graduate Theses and Dissertations

The effect of Spirulina platensis inclusion in feed of commercial broilers was evaluated over one experiment. The study aimed to investigate the effects of Spirulina platensis freshwater algae inclusion on live performance and processing characteristics of commercial broilers subjected to daily cyclic heat stress. Day old Ross 708 male broilers were placed into 8 environmentally controlled chambers. At d 21, four chambers remained on a control diet containing no algae (CON) while 4 chambers received a diet that included algae at an inclusion rate of 2.5% (ALG). An 8 hour daily cyclic heat stress (24°C to 36°C) was applied to …

Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta

Graduate Theses and Dissertations

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …

Identification Of Possible Vectors For Histomoniasis In Turkeys On Commercial Farms In Arkansas And Oklahoma, Treva Dillon

Graduate Theses and Dissertations

Histomonas meleagridis, is a parasitic protozoan, commonly found in the gallinaceous species of birds and is the causative agent of Blackhead disease. The cecal worm, known as Heteraskis gallinarum, acts as a vector in the spread of H. meleagridis in gallinaceous birds. H. meleagridis develops inside the cecal worm and in its eggs which allows H. meleagridis a method to survive in the environment. Blackhead disease causes high mortality rates in both chickens and turkeys; however, turkeys are noted to have a higher mortality rate compared to chickens. Molecular diagnostic testing such as PCR has been developed by creating primers …

Optimization Of Clostridium Septicum Antigen Production And Evaluation Of Vaccine Administration Parameters For A Candidate Bacterin-Toxoid To Prevent Dermatitis In Commercial Turkeys, Aaron Forga

Graduate Theses and Dissertations

The objective of this thesis was to optimize the hemolytic activity of two isolates of Clostridium septicum to evaluate if hemolytic titer of C. septicum antigen at time of formalin inactivation corresponded to increased serum antibody titer to the C. septicum alpha-toxin of turkeys immunized with an experimental formalin inactivated bacterin-toxoid vaccine. Chapter 1 provides a brief overview of the thesis. Chapter 2 consists of a literature review focused on clostridial dermatitis, specifically C. septicum-associated dermatitis (cellulitis) that affects commercial turkeys. Chapter 3 outlines our attempts to optimize an experimental autogenous bacterin-toxoid vaccine as a potential strategy to mitigate clostridial …

Impacts On Fast-Start Performance: How Do Group Size And Habitat Degradation Alter The Escape Behavior Of A Schooling Coral Reef Fish?, Monica D. Bacchus

All HCAS Student Capstones, Theses, and Dissertations

Escaping predation is essential for species survival, but prey must effectively match their response to the perceived threat imposed by a predator. Fish evaluate their surroundings using several sensory stimuli, including olfactory, visual, auditory, and mechanical cues. A range of taxa use the fast-start response to evade predators, including fishes, sharks, and larval amphibians. While the fast-start response (rapid bursts of swimming) is extensively studied in solitary fishes, the factors that mediate the collective escape response in schools of fish have historically been investigated far less. To address this knowledge gap, the collective escape behavior and individual escape performance of …

Effects Of An Injectable Zinc Solution At Weaning As An Alternative Castration Method In Beef Cattle, Reagan Nicole Cauble

Graduate Theses and Dissertations

Two experiments were conducted to investigate a 1 mL intratesticular zinc (100 mg Zn) injection administered into each testicle at weaning (Z) and its effects on growth, health, behavior and testosterone. In the first experiment, beef bulls were assigned randomly to treatment at birth: 1) surgically castrated at birth (S; n = 37) or 2) Z (n = 37). Testicular thickness differed by day (P < 0.01) for Z. Testosterone concentrations were greater in Z by d 77 and remained so through d 280 (P = 0.02) compared to S. Zinc injected calves were heavier at the trail’s conclusion (P ≤ 0.04), had greater ADG overall (P < 0.01), heavier hot carcass weight (P = 0.01), and greater lean muscle area (P = 0.01) compared to S; but a lower marbling score (P < 0.01). Yield Grade, dressed carcass yield and fat thickness were similar (P ≥ 0.14). Zinc injected calves had greater haptoglobin (Hp) concentrations (P < 0.01). Zinc injected calves had greater concentrations of white blood cells on d 1 and 2 and greater concentrations and proportions of neutrophils on d 1, 2 and 3 (P < 0.01). Conversely, S had a greater percentage of lymphocytes on d 1, 2 and 3 and a lower ratio of neutrophils to lymphocytes during that same time (P < 0.01). Zinc injected calves spent more time on their side or sternum on d 1 and more time on their side on d 2 while S spend more time standing during that time (P < 0.01). During d 3, 4, 5, and 6, Z stood more while S laid on their sternum (P < 0.01). In trail 2, beef bulls were allocated randomly to treatment one week post weaning: 1) banded (B; n = 42) or 2) Z (n = 39). Body weights were similar (P ≥ 0.39) but ADG improved for Z compared to B (P = 0.05). Testosterone concentrations were greater in Z compared to B (P ≤ 0.02). Testicular width in Z differed by d (P < 0.01). Zinc castrated calves spent more time on their side compared to B one day post castration (P = 0.03) until d 3 and 4 where B spent more time on their side (P ≤ 0.02). Banded calves stood more the first two days (P ≤ 0.01). On days 3, 4, 5, and 6, Z stood more (P ≤ 0.01) while B were on their sternum (P ≤ 0.03). Banded calves had lower Hp concentrations compared to Z (P ≤ 0.01). Total white blood cell concentrations, proportions and concentrations of neutrophils, and proportion of lymphocytes were greater in Z compared to B on d 1, 2, and 3 (P ≤ 0.01). There was no interaction between treatments over time for IL1β, IL6, and TNFα expression (P ≥ 0.83). The findings in both studies indicate that intratesticular Zn injections at weaning can improve growth performance and some carcass attributes but produce a heightened inflammatory and immune response and cause discomfort. Injecting zinc, as formulated and administered in these trails, does not result in complete castration of beef calves at this age and therefore cannot be considered a true castration alternative.

Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole Mckinney

Graduate Theses and Dissertations

Poultry is a staple protein source for most of the planet. Until recently, antibiotic growth promoters (AGPs) were used to prevent illnesses in commercial chicken production. Currently, this is not possible due to regulations and consumer concern, but without such a preventative, diseases like necrotic enteritis (NE) have reemerged, posing a threat to bird health, and ultimately, our food source. Necrotic enteritis is a severe gastrointestinal disease caused by the gram-positive pathogen, Clostridium perfringens. Clinical features of this disease are diarrhea, intestinal lesions, and death, with a high transmission rate. In a subclinical form, growth performance is diminished and is …

Effect Of Sensory Additives On Sow Lactation Performance, Subsequent Reproductive Performance, And Nursey Pig Performance, Nathan E. Davis

Graduate Theses and Dissertations

Maximizing nutrient intake during lactation is vital to sow performance as well as litter performance. The aim of these studies was to evaluate the impact of a sensory additive on sow lactation performance and nursery pig performance. Five groups of sows were farrowed, three during winter (October, December and January) and two during summer months (June, July, August). Sows were weighed, and back fat depth was measured, at 110 d of gestation prior to entering the lactation room, and again at weaning. The sows were blocked by parity, and then allotted by BW at d 110 to Control or 0.075% …

The Role Of Microbiota On Campylobacter Jejuni Colonization And Growth Performance In Broiler Chickens, Ayidh Almansour

Graduate Theses and Dissertations

The successful poultry production at modern era comes from the vertical integratedindustrialization, which has fundamentally changed how animals have been living for millions of years. Antimicrobial growth promoters have been used to sustain the efficient industrialized animal production, driving antimicrobial overuse and resistance. Because of the increasing pressure from consumer’s concerns and government regulations, it is urgent to develop antimicrobial free alternatives as growth promoters in poultry production, but few effective antimicrobial alternatives are currently available. Campylobacter jejuni is one of the worldwide prevalent foodborne bacterial pathogens mainly transmitted from poultry. However, few mechanisms are available on why C. jejuni …

Investigating Molecular Mechanisms Behind Bacterial Chondronecrosis With Osteomyelitis (Bco) Pathogenesis In Modern Broilers, Alison Ramser

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis (BCO), a leading cause of lameness in broiler chickens, is characterized by infection, inflammation, and bone attrition. There are currently no effective treatments and positive diagnosis is only possible through necropsy evaluations. Lameness is also a rising animal welfare and economic concern, making prevention and detection of BCO all the more critical. These challenges are exacerbated by a lack of mechanistic understanding of BCO’s etiology. The question I asked during my dissertation was how bacteria induce bone attrition in BCO pathology. My research has shown that mitochondrial dysfunction is characteristic of BCO conditions along with autophagy …

Pandemics And Animal Welfare: A Quantitative Inquiry Into How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Kayla N. Desmet

Animal Science Undergraduate Honors Theses

Since the emergence of the COVID-19 pandemic in late 2019, industries have been impacted globally. The companion animal care industry has not received much recognition in mainstream news, and though multiple grants have been allocated (Nolen, 2020), limited resources were available to individual facilities such as animal shelters, animal day cares, and boarding facilities. Little is known about the long-term effects of the pandemic on these facilities and how companion animal care may have changed. The purpose of this study was to explore the day-to-day impacts and long-term effects of COVID-19 on the companion animal industry in Northwest Arkansas from …

The Effect Of Visitor Density And Interaction On The Behavior Of Four Ray Species (Hypanus Sabina, Hypanus Say, Pseudobatos Lentiginosus, And Rhinoptera Bonasus) Housed In An Aquatic Touch Pool, Aimee Marie Little

UNF Graduate Theses and Dissertations

Human-Animal Interactions (HAI) in zoological institutions are thought to be important in helping visitors to establish a connection with animals and thus making them more likely to contribute to conservation efforts. However, animals can respond to visitor interaction in both negative and positive ways. The growing focus on animal welfare in zoological institutions emphasizes the need for assessing different environmental inputs, including visitor interaction, and how these inputs influence behavioral outputs associated with welfare. A touch pool exhibit presents a novel interactive experience that allows visitors to directly interact with various aquatic species, including elasmobranchs, whose conservation has important implications …

A Comparison Of Chimpanzee Wound Rates Before And During Covid-19 Zoo Closures, Robin Elana Salak

All Master's Theses

Visitor effect studies have had inconsistent impacts on animals, due in part to the inability to control for all confounding variables such as time of day, seasonal weather patterns, and so forth. This study represents the first instance where chimpanzee (Pan troglodytes) wounding frequencies were investigated across extensive time periods in the presence and complete absence of visitors, thus eliminating many visitor-related variables. Additional variables were eliminated through the zoo selection process, based on institutional responses to a 29-question survey, providing a novel approach to the question of visitor effects. The aim of this study was to determine …

Pushing It To The Limit: Determining Asian Elephant (Elephas Maximus) Olfactory Sensitivity And Discrimination Through A Behavioral Choice Task, Matthew S. Rudolph

Theses and Dissertations

Elephants have shown remarkable olfactory capabilities. Their sense of smell impacts their foraging choices, behavior, and ultimately, survival. Being able to detect a target odor can allow elephants to locate specific resources, identify threats, and find receptive conspecifics. Previous studies have shown that elephants can consistently detect target odors, but have not identified the limits of this detection. Thus, to investigate the extent of elephants’ odor detection capabilities, we tested Asian elephants in a two-step odor discrimination task. First, we investigated whether elephants could detect odors at varying levels of dilution after a training procedure, and then whether they could …

The Art Of Amphibian Conservation: Linking In-Situ And Ex-Situ Populations Of Endangered Species Through Genome Banking, Isabella Joann Burger

Theses and Dissertations

Limited breeding success in captive breeding programs has necessitated the development of assisted reproductive technologies (ART) to preserve and increase genetic variation and population numbers of both captive and wild amphibian groups. ART has been shown to be successful in numerous anuran species, and current studies focus on the application of ART in ex-situ populations. The focus of this project is to show that linking in-situ and ex-situ amphibian populations through sperm cryopreservation, genome banking, and in-vitro fertilization is possible, with the goal of increasing gene diversity throughout groups in order to produce self-sustaining, wild populations in the future. Specific …

Forest Disturbance And Occupancy Patterns Of Carnivores: Results Of A Large-Scale Field Study In Maine, Usa, Bryn E. Evans

Electronic Theses and Dissertations

Understanding trends in the abundance and distribution of carnivores is important at global, regional and local scales due to their ecological role, their aesthetic and economic value, and the numerous threats to their populations. Carnivores in Maine range from the American black bear (Ursus americanus), to numerous native mesocarnivore species, such as American marten (Martes americana), fisher (Pekania pennanti), coyote (Canis latrans), red fox (Vulpes vulpes), bobcat (Lynx rufus), Canada lynx (Lynx canadensis) and to two small weasel species (Mustela erminea and Neogale frenata). …