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Full-Text Articles in Plant Pathology
A Pseudomonas Syringae Pv. Tomato Dc3000 Mutant Lacking The Type Iii Effector Hopq1-1 Is Able To Cause Disease In The Model Plant Nicotiana Benthamiana, Chia-Fong Wei, Brian H. Kvitko, Rena Shimizu, Emerson Crabill, James R. Alfano, Nai-Chun Lin, Gregory B. Martin, Hsiou-Chen Huang, Alan Collmer
A Pseudomonas Syringae Pv. Tomato Dc3000 Mutant Lacking The Type Iii Effector Hopq1-1 Is Able To Cause Disease In The Model Plant Nicotiana Benthamiana, Chia-Fong Wei, Brian H. Kvitko, Rena Shimizu, Emerson Crabill, James R. Alfano, Nai-Chun Lin, Gregory B. Martin, Hsiou-Chen Huang, Alan Collmer
Center for Plant Science Innovation: Faculty and Staff Publications
The model pathogen Pseudomonas syringae pv. tomato DC3000 causes bacterial speck in tomato and Arabidopsis, but Nicotiana benthamiana, an important model plant, is considered to be a non-host. Strain DC3000 injects approximately 28 effector proteins into plant cells via the type III secretion system (T3SS). These proteins were individually delivered into N. benthamiana leaf cells via T3SS-proficient Pseudomonas fluorescens, and eight, including HopQ1-1, showed some capacity to cause cell death in this test. Four gene clusters encoding 13 effectors were deleted from DC3000: cluster II (hopH1, hopC1), IV (hopD1, hopQ1-1, hopR1), IX (hopAA1-2, hopV1, hopAO1, hopG1), and native plasmid pDC3000A …