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Full-Text Articles in Plant Pathology
Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby
Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby
Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications
Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …