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Safety Evaluations Of The Cry1ia1 Protein Found In The Transgenic Potato 'Spuntag2', Hector Quemada, Kelly Zarka, Walter Pett, Gurling Bothma
Safety Evaluations Of The Cry1ia1 Protein Found In The Transgenic Potato 'Spuntag2', Hector Quemada, Kelly Zarka, Walter Pett, Gurling Bothma
University Faculty Publications and Creative Works
The transgenic potato 'SpuntaG2' (Solanum tuberosum), which is resistant to potato tuber moth (Phthorimaea operculella), was subjected to protein safety evaluations including protein equivalency tests for the Cry1Ia1 protein from 'SpuntaG2' and bacterially produced Cry1Ia1, toxicity and allergenicity evaluations of Cry1Ia1 protein, and compositional equivalency of 'SpuntaG2' compared with non-transgenic 'Spunta'. Western blot analysis and biological activity assays showed molecular and functional equivalency between 'SpuntaG2'-derived Cry1Ia1 protein and bacteria-derived Cry1Ia1 protein. Comparison of the Cry1Ia1 amino acid sequence to known amino acid sequences revealed no significant homology to known toxins or known allergens. Acute toxicity studies using rodents were used …