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Full-Text Articles in Agronomy and Crop Sciences

‘Forrest’ Resistance To The Soybean Cyst Nematode Is Bigenic: Saturation Mapping Of The Rhg1 And Rhg4 Loci, K. Meksem, P. Pantazopoulos, V. N. Njiti, D. L. Hyten, P. R. Arelli, D. A. Lightfoot Oct 2001

‘Forrest’ Resistance To The Soybean Cyst Nematode Is Bigenic: Saturation Mapping Of The Rhg1 And Rhg4 Loci, K. Meksem, P. Pantazopoulos, V. N. Njiti, D. L. Hyten, P. R. Arelli, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv ‘Forrest’ is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of Forrest × Essex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg …


Intsormil 2001 Annual Report, John M. Yohe, Thomas Crawford Jr., Joan Frederick, Dorothy Stoner Jan 2001

Intsormil 2001 Annual Report, John M. Yohe, Thomas Crawford Jr., Joan Frederick, Dorothy Stoner

INTSORMIL Impacts and Bulletins

Presently, worldwide, more than 800 million people are hungry and over one billion are desperately poor, and food demand is increasing rapidly. The majority of poor live in rural areas in developing countries and agricultural and food systems development is vital to economic growth; improving environmental quality; strengthening nutrition, health and child survival; improving the status of women; and promoting democratization. It is estimated that, between 1980 and 2030, the population ofow- and middle-income countries will more than double-to seven billion, compared with one billion for high-income countries. In the next 35 years, 2.5 billion people will be added to …


Arthropod Populations And Rice Yields In Direct-Seeded And Transplanted Lowland Rice In West Africa, I. O. Oyediran, E. A. Heinrichs Jan 2001

Arthropod Populations And Rice Yields In Direct-Seeded And Transplanted Lowland Rice In West Africa, I. O. Oyediran, E. A. Heinrichs

Department of Entomology: Faculty Publications

Two methods for planting rice in irrigated lowland were evaluated during the wet seasons of 1994 and 1995 to determine their effect on rice arthropod numbers, insect-caused rice plant damage, and rice grain yield. The six treatments tested were: hand transplanting of seedlings at spacings of 14 cm × 14 cm, 20 cm × 20 cm, and 30 cm × 30 cm; and direct-seeding of rice at 60 kg seeds ha–1, 90 kg seeds ha–1, and 120 kg seeds ha–1. The most abundant arthropods in the study were the diopsid flies, Diopsis longicornis Maquart …


Manure Matters, Volume 7, Number 1, J. P. Harner, T. D. Strahm, D. Key, T. L. Strahm, J.P. Murphy Jan 2001

Manure Matters, Volume 7, Number 1, J. P. Harner, T. D. Strahm, D. Key, T. L. Strahm, J.P. Murphy

Manure Matters (newsletter)

Time and motion (TM) studies are useful in determining labor requirement to perform a specific task in manufacturing processes. These studies have been used in the dairy industry to evaluate the labor requirements for various tasks associated with milking routine and milk parlor. Data may be used to evaluate the impact of changes or help in understanding labor requirements. The objective of this project was to determine the labor requirements for removing manure from concrete basins prior to land applications.


Manure Matters, Volume 7, Number 3, M. S. Davis, Terry L. Mader Jan 2001

Manure Matters, Volume 7, Number 3, M. S. Davis, Terry L. Mader

Manure Matters (newsletter)

Using Weather Stations in Nebraska Livestock Operations

Enforcement of environmental regulations placed on feedlots in Nebraska by the Environmental Protection Agency (EPA), have lead to an increased need for accurate documentation of weather variables. In addition to the need to monitor weather conditions for compliance, an accurate representation of current weather conditions within the microclimate of the livestock operation allow for proactive responses to periods of potential environmental stress periods (i.e. heat and cold stress). Currently, most producers use manual documentation of rainfall events, local weather forecasts and some degree of intuition to monitor weather conditions. Weather station technology exists …


Manure Matters, Volume 7, Number 4, Raymond Ward Jan 2001

Manure Matters, Volume 7, Number 4, Raymond Ward

Manure Matters (newsletter)

How to Read a Manure Sample Report

Correctly sampling manure can be a challenge. Try to obtain a representative sample and get the sample to the lab as close as possible to the time when manure is going to be applied. It is preferable to obtain sample results before land application so that appropriate application rates can be determined.

Slurry samples should be taken at the time the slurry is being applied, so that you have a good mix of the manure storage. The amount of material is usually recorded in gallons per acre and once the analysis is received, …


Manure Matters, Volume 7, Number 5 Jan 2001

Manure Matters, Volume 7, Number 5

Manure Matters (newsletter)

Marketing Manure-Part 1

This is part 1 of a two part series discussing the results of a manure marketing survey conducted by the University of Nebraska.
Livestock enterprises have undergone substantial structural change in recent years, including the concentration of livestock and poultry industry into fewer farms (Council for Agricultural Science and Technology, 1996). There has been a reduction in farms with cattle by 50% and farms with swine by 80% between 1965 and 1995 while numbers of hogs and cattle in inventory has stayed nearly constant (USDA, 1965-1995). Livestock and poultry produce, annually, 3.5 and 3.1 million tons of …


Manure Matters, Volume 7, Number 8, Richard Stowell Jan 2001

Manure Matters, Volume 7, Number 8, Richard Stowell

Manure Matters (newsletter)

Pit Additives

As producers have been trying to deal with odor issues on their operations, many have been inundated with product advertisements and solicitations for pit additives. Understandably, producer concerns arise over product effectiveness, reliability and value. Results of an NPPC-supported study on the effectiveness of many pit additives were released this year. This article summarizes major findings of that Odor Solutions Initiative study. A full report of results from that study is available through NPPC.


Manure Matters, Volume 7, Number 2, Rick Koelsch Jan 2001

Manure Matters, Volume 7, Number 2, Rick Koelsch

Manure Matters (newsletter)

Pivot Irrigation of Livestock Manure
The selection of an appropriate land application method for manure can have an impact on several environmental issues. Individual methods should be evaluated based upon impact on:

• Air quality
• Water quality
• Soil conservation and quality
• Pathogen transmission

The following discussion will review these considerations as they relate to application of manure or lagoon effluent through a center pivot system. A generalized comparison of the relative strengths and weaknesses of alternative application methods is attached in Table 1.


Manure Matters, Volume 7, Number 10, Christopher Henry, Richard K. Koelsch Jan 2001

Manure Matters, Volume 7, Number 10, Christopher Henry, Richard K. Koelsch

Manure Matters (newsletter)

What is an Anaerobic Digester?

The first methane digester plant was built at a leper colony in Bombay, India in 1859. A methane digester system, commonly referred to as an AD (anaerobic digester) is a device that promotes the decomposition of manure or “digestion” of the organics in manure to simple organics and gaseous biogas products. Manure is regularly put into the digester after which the microbes break down the manure into biogas and a digested solid. The digested manure is then deposited into a storage structure. The biogas can be used in an engine generator or burned in a …


Manure Matters, Volume 7, Number 7, J. David Aiken Jan 2001

Manure Matters, Volume 7, Number 7, J. David Aiken

Manure Matters (newsletter)

Nebraska Livestock Nuisance Law

Until 1976 the Nebraska Supreme Court had ruled that animal feeding operations (AFOs) located in rural areas did not legally constitute a nuisance unless the AFO were improperly operated. However, in 1976 the Nebraska Supreme Court ruled that a rural AFO may constitute a nuisance even if properly operated. This decision made AFO operators liable for the first time in Nebraska if their AFO constituted a nuisance.
In 1977 the Nebraska Unicameral adopted an amendment to the Nebraska Environmental Policy Act defining when AFOs would legally constitute a nuisance. Basically, the statute said that an AFO …


Manure Matters, Volume 7, Number 6, Keith Glewen, Rick Koelsch Jan 2001

Manure Matters, Volume 7, Number 6, Keith Glewen, Rick Koelsch

Manure Matters (newsletter)

Marketing Manure - Part 2

This is part 2 of a two part series discussing the results of a manure marketing survey conducted by the University of Nebraska.
The survey attempted to identify those services that were packaged with the export of manure to off-farm customers. At this time, many producers do not offer any services to enhance the value of manure (Table 4). Of those exporting manure to off farm users, 40% offered no agronomic services, 51% provided no nuisance avoidance services, and 70% offered no manure processing services. However, there were a number of feedlots that offered services …


Manure Matters, Volume 7, Number 9, Richard K. Koelsch, Bryan Woodbury, David Stenberg, Daniel N. Miller, Dennis Schulte Jan 2001

Manure Matters, Volume 7, Number 9, Richard K. Koelsch, Bryan Woodbury, David Stenberg, Daniel N. Miller, Dennis Schulte

Manure Matters (newsletter)

Total Reduced Sulfur Concentration in Beef Cattle Feedlots

In 1997, the Nebraska Department of Environmental Quality amended its Title 129 Air Quality Regulations to establish a regulatory threshold for Total Reduced Sulfur (TRS) concentrations under ambient conditions. These thresholds are set at “10.0 parts per million (10.0 PPM) maximum 1 minute average concentration or 0.10 parts per million (0.10 PPM) maximum 30-minute rolling average”. Two agricultural regions of Nebraska with significant cattle finishing in open feedlots came under scrutiny for possible rule violations. In one situation, area feedlots were asked to prepare and implement a TRS control plan.

This growing …


Composting Of Feedlot And Dairy Manure: Compost Characteristics And Impact On Crop Yields, Galen E. Erickson, Terry J. Klopfenstein, Walker Luedtke, Mark Schroeder, Charles A. Francis, Gary Lesoing Jan 2001

Composting Of Feedlot And Dairy Manure: Compost Characteristics And Impact On Crop Yields, Galen E. Erickson, Terry J. Klopfenstein, Walker Luedtke, Mark Schroeder, Charles A. Francis, Gary Lesoing

Nebraska Beef Cattle Reports

Since 1993, approximately 17,600 tons of beef feedlot and dairy compost have been spread on 1,100 acres. Crop yields were measured to determine the impact of a one-time compost application by using no-compost check strips in large-scale production fields. Adding compost to irrigated corn, irrigated soybeans, and dryland corn acres significantly increased yields, with four-year average increases of 2.3, 1.5, and 2.7%, respectively. For all crops measured, the response to compost was greatest the first year following application and declined linearly in subsequent years. The increased yield from compost application offsets spreading costs using average prices for crops.


Genetic Analysis Of Kafirins And Their Phenotypic Correlations With Feed Quality Traits, In Vitro Digestibility, And Seed Weight In Grain Sorghum, C. Hicks, S. R. Bean, G. L. Lookhart, Jeffrey F. Pedersen, K. D. Kofoid, M. R. Tuinstra Jan 2001

Genetic Analysis Of Kafirins And Their Phenotypic Correlations With Feed Quality Traits, In Vitro Digestibility, And Seed Weight In Grain Sorghum, C. Hicks, S. R. Bean, G. L. Lookhart, Jeffrey F. Pedersen, K. D. Kofoid, M. R. Tuinstra

Department of Agronomy and Horticulture: Faculty Publications

Twenty-three entries of grain sorghum (Sorghum bicolor (L.) Moench), including eight inbred lines (five males and three females) and 15 hybrids, were evaluated to determine the proportion of γ, αII, and β-αI-kafirins and their association with contents of crude protein, fat, and starch; protein digestibility; in vitro dry matter disappearance; and seed weight. The male lines included three normal-seeded lines (TX2737, TX435, and P954063) and two large-seeded lines (Eastin1 and PL-1). Female lines consisted of three common U.S. seed parent lines (Wheatland, Redlan, and SA3042). The lines and their hybrids were grown under dryland conditions at two locations in …


Conversion Of Aflp Bands Into High-Throughput Dna Markers, K. Meksem, E. Ruben, D. L. Hyten, K. Triwitayakorn, D. A. Lightfoot Jan 2001

Conversion Of Aflp Bands Into High-Throughput Dna Markers, K. Meksem, E. Ruben, D. L. Hyten, K. Triwitayakorn, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

The conversion of AFLP bands into polymorphic sequence-tagged-site (STS) markers is necessary for high-throughput genotype scoring. Technical hurdles that must be overcome arise from genome complexity (particularly sequence duplication), from the low-molecular- weight nature of the AFLP bands and from the location of the polymorphism within the AFLP band. We generated six STS markers from ten AFLP bands (four AFLPs were from co-dominant pairs of bands) in soybean (Glycine max). The markers were all linked to one of two loci, rhg1 on linkage group G and Rhg4 on linkage group A2, that confer resistance to the soybean cyst nematode (Heterodera …


Genomic Regions That Underlie Soybean Seed Isoflavone Content, K. Meksem, V. N. Njiti, W. J. Banz, M. J. Iqbal, My. M. Kassem, David L. Hyten, J. Yuang, T. A. Winters, D. A. Lightfoot Jan 2001

Genomic Regions That Underlie Soybean Seed Isoflavone Content, K. Meksem, V. N. Njiti, W. J. Banz, M. J. Iqbal, My. M. Kassem, David L. Hyten, J. Yuang, T. A. Winters, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Soy products contain isoflavones (genistein, daidzein, and glycitein)that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL)fr om the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast for isoflavone content. Isoflavone content of seeds fromeach RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The …


Seed Physiology, Production & Technology: Germination, Emergence, And Yield Of 20 Plant-Color, Seed-Color Near-Isogenic Lines Of Grain Sorghum, Jeffrey F. Pedersen, J. J. Toy Jan 2001

Seed Physiology, Production & Technology: Germination, Emergence, And Yield Of 20 Plant-Color, Seed-Color Near-Isogenic Lines Of Grain Sorghum, Jeffrey F. Pedersen, J. J. Toy

Department of Agronomy and Horticulture: Faculty Publications

Although there is growing demand for sorghum [Sorghum bicolor (L.) Moench] with white seed and tan plant color, there is limited information on the overall agronomic fitness of sorghum with these characters. A set of experiments was conducted to evaluate the combined effects of plant color and seed color on sorghum germination, emergence, and agronomic performance. Twenty near-isogenic lines with red seed/tan plant (RT), red seed/purple plant (RP), white seed/ tan plant (WT), white seed/purple plant (WP) phenotypes were tested under field and laboratory conditions. Plant color X seed color interactions were not significant. Purple plant color phenotypes had higher …


High-Throughput Genotyping For A Polymorphism Linked To Soybean Cyst Nematode Resistance Gene Rhg4 By Using TaqmanTm Probes, Khalid Meksem, Eliza Ruben, David L. Hyten, Michael E. Schmidt, David A. Lightfoot Jan 2001

High-Throughput Genotyping For A Polymorphism Linked To Soybean Cyst Nematode Resistance Gene Rhg4 By Using TaqmanTm Probes, Khalid Meksem, Eliza Ruben, David L. Hyten, Michael E. Schmidt, David A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

An individual soybean breeder can generate over one hundred thousand new genotypes each year. The efficiency of selection in these populations could be improved if these genotypes were effectively screened with one DNA marker that identified an important gene, and if laboratory throughput was high and costs were low. Our aim was to develop a rapid genotyping procedure for resistance to the soybean cyst nematode. A high-throughput genotyping method was developed with fluorogenic probes to distinguish between two insertion polymorphisms in alleles of an AFLP marker that is located about 50 kbp from the Rhg4 gene candidate. The assay uses …