Agronomy and Crop Sciences Commons^™

Perennializing Marginal Croplands: Going Back To The Future To Mitigate Climate Change With Resilient Biobased Feedstocks, Salvador Ramirez Ii, Marty R. Schmer, Virginia L. Jin, Robert B. Mitchell, Catherine E. Stewart, Jay Parsons, Daren D. Redfearn, John J. Quinn, Gary E. Varvel, Kenneth P. Vogel, Ronald F. Follett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Managing annual row crops on marginally productive croplands can be environmentally unsustainable and result in variable economic returns. Incorporating perennial bioenergy feedstocks into marginally productive cropland can engender ecosystem services and enhance climate resiliency while also diversifying farm incomes. We use one of the oldest bioenergy-specific field experiments in North America to evaluate economically and environmentally sustainable management practices for growing perennial grasses on marginal cropland. This long-term field trial called 9804 was established in 1998 in eastern Nebraska and compared the productivity and sustainability of corn (Zea mays L.)—both corn grain and corn stover—and switchgrass (Panicum virgatum …

Description Of Baetao-Manteiga 41 And ‘Yunguilla’ Superior Andean Common Beans For Tanzanian Production Environments, S. Nchimbi Msolla, Phillip N. Miklas, D. Fourie, M. Kilango, T. Porch

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An international effort was initiated with the evaluation of a diverse set of largeseeded Andean common beans (Phaseolus vulgaris L.), the Andean Diversity Panel, in sub-Saharan Africa. Several entries in the panel have been selected for extensive characterization on the basis of high performance acrossmultiple location × year trials in Tanzania—conducted both on station and on farm. Baetao- Manteiga 41, tested asADP-190, has a commercialKablanketi (light purple speckled) seed type, and ‘Yunguilla’, tested as ADP-447, has a commercial Calima (red mottled) seed type. Both lines exhibited yield stability, vigorous growth under low fertility conditions, angular leaf spot resistance, andmoderate …

Contributions In Puerto Rico To Bean, Phaseolus Spp., Research, James S. Beaver, Consuelo Estévez De Jensen, Phillip N. Miklas, Timothy G. Porch

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Most literature reviews focus on a specific topic. The purpose of this paper, however, is to review the contributions made by a multidisciplinary team of researchers at a specific location (Puerto Rico) over a period of several decades. This paper documents bean research of the University of Puerto Rico and the USDA-ARS Tropical Agriculture Research Station during the past century. The following illustrates the merits of continuity of effort in research and shows that research often follows a logical sequence of actions to solve problems related to genetic improvement as well as to study biotic and abiotic factors that affect …

Genetic Diversity Of Aflatoxin-Producing Aspergillus Flavus Isolated From Selected Groundnut Growing Agro-Ecological Zones Of Uganda, Amos Acur, Renee Arias, Steven Odongo, Samuel Tuhaise, National Agricultural Research Laboratories, Kampala, Uganda, John Adriko, Dennis Muhanguzi, Stephen Buah, Andrew Kiggundu

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Groundnut pre- and post-harvest contamination is commonly caused by fungi from the Genus Aspergillus. Aspergillus flavus is the most important of these fungi. It belongs to section Flavi; a group consisting of aflatoxigenic (A. flavus, A. parasiticus and A. nomius) and non-aflatoxigenic (A. oryzae, A. sojae and A. tamarii) fungi. Aflatoxins are food-borne toxic secondary metabolites of Aspergillus species associated with severe hepatic carcinoma and children stuntedness. Despite the well-known public health significance of aflatoxicosis, there is a paucity of information about the prevalence, genetic diversity and population structure of A. flavus in different …

Irrigated Pinto Bean Crop Stress And Yield Assessment Using Ground Based Low Altitude Remote Sensing Technology, Rakesh Ranjan, Abhilash K. Chandel, Lav R. Khot, Haitham Y. Bahlol, Jianfeng Zhou, Rick A. Boydston, Phillip N. Miklas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The pinto bean is one of widely consumed legume crop that constitutes over 42% of the U.S dry bean production. However, limited studies have been conducted in past to assess its quantitative and qualitative yield potentials. Emerging remote sensing technologies can help in such assessment. Therefore, this study evaluates the role of ground-based multispectral imagery derived vegetation indices (VIs) for irrigated the pinto bean stress and yield assessments. Studied were eight cultivars of the pinto bean grown under conventional and strip tillage treatments and irrigated at 52% and 100% of required evapotranspiration. Imagery data was acquired using a five-band multispectral …

The Role Of Genotype And Production Environment In Determining The Cooking Time Of Dry Beans (Phaseolus Vulgaris L.), Karen A. Cichy, Jason A. Wiesinger, Matthew Berry, Susan Nchimbi‐Msolla, Deidre Fourie, Timothy G. Perch, Daniel Ambechew, Phillip N. Miklas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dry bean (Phaseolus vulgaris L.) is a nutrient‐dense food rich in proteins and minerals. Although a dietary staple in numerous regions, including Eastern and Southern Africa, greater utilization is limited by its long cooking time as compared with other staple foods. A fivefold genetic variability for cooking time has been identified for P. vulgaris, and to effectively incorporate the cooking time trait into bean breeding programs, knowledge of how genotypes behave across diverse environments is essential. Fourteen bean genotypes selected from market classes important to global consumers (yellow, cranberry, light red kidney, red mottled, and brown) were grown …

Suppression Of Aflatoxin Production In Aspergillus Species By Selected Peanut (Arachis Hypogaea) Stilbenoids, Victor S. Sobolev, R. S. Arias, Kerestin Goodman, Travis Walk, Valerie Orner, Paola C. Faustinelli, Alicia Massa

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aspergillus flavus is a soil fungus that commonly invades peanut seeds and often produces carcinogenic aflatoxins. Under favorable conditions, the fungus-challenged peanut plant produces and accumulates resveratrol and its prenylated derivatives in response to such an invasion. These prenylated stilbenoids are considered peanut antifungal phytoalexins. However, the mechanism of peanut−fungus interaction has not been sufficiently studied. We used pure peanut stilbenoids arachidin-1, arachidin-3, and chiricanine A to study their effects on the viability of and metabolite production by several important toxigenic Aspergillus species. Significant reduction or virtually complete suppression of aflatoxin production was revealed in feeding experiments in A. flavus, …

The Lx Report Of The Bean Improvement Cooperative No. 60, March 2017.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CONTENTS

LX Annual Report of the Bean Improvement Cooperative xi

BIC Coordinating Committee Membership - 1957 to 2017 . xii

BIC Meritorious Service and Distinguished Achievement Award Recipients - 1957 to 2015 xiii

BIC Awards Nomination Request for 2017 xvi

BIC/NAPIA Meeting in 2017 . xix

In Memory of Guillermo E. Galvez-Enriquez xx

RESEARCH PAPERS FOR 2017

OPTIMIZING SPORULATION OF Pseudocercospora griseola IN VITRO • Paula F. de Pádua1, Rafael Pereira1, Luanna B. W. Gomes1 and Elaine A. de Souza1* 1

FINE MAPPING THE BROAD SPECTRUM ANTHRACNOSE RESISTANCE GENE IN AMENDOIM CAVALO 3 • Gilio, T.A.S.1, Oscar P. Hurtado-Gonzales2, Giseli …

Characterization Of Small Rna Populations In Non-Transgenic Andaflatoxin-Reducing-Transformed Peanut, Imana L. Power, Phat M. Dang, Victor S. Sobolev, Valerie Orner, Joseph L. Powell, Marshall C. Lamb, Renée S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aflatoxin contamination is a major constraint in food production worldwide. In peanut (Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumula-tion of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNApopulations in a control line and in two transformed peanut lines that expressed an inverted repeattargeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B1 than the controls. The objective was …

Chemical Interruption Of Late Season Flowering To Improve Harvested Peanut Maturity, Marshall C. Lamb, Ronald B. Sorensen, Christopher L. Butts, Phat M. Dang, C. Y. Chen, Renée S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peanut (Arachis hypogaea) is a botanically indeterminate plant where flowering, fruit initiation, and pod maturity occurs over an extended time period during the growing season. As a result, the maturity and size of individual peanut pods vary considerably at harvest. Immature kernels that meet commercial edible size specifications negatively affect quality during processing due to their increased propensity for off flavors, higher moisture and water activity, and variable roasting properties. As peanuts progress toward maturation, late season flowers set within 40 days till harvest will not have sufficient time to develop into mature, marketable pods prior to harvest. …

Aspergillus And Aflatoxin In Groundnut (Arachis Hypogaea L.) And Groundnut Cake In Eastern Ethiopia, Abdi Mohammed, Alemayehu Chala, Mashilla Dejene, Chemeda Fininsa, David A. Hoisington, Victor S. Sobolev, R. S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study was conducted to assess major Aspergillus species and aflatoxins associated with groundnut seeds and cake in Eastern Ethiopia and evaluate growers’ management practices. A total of 160 groundnut seed samples from farmers’ stores and 50 groundnut cake samples from cafe and restaurants were collected. Fungal isolation was done from groundnut seed samples. Aspergillus flavus was the dominant species followed by Aspergillus parasiticus. Aflatoxin analyses of groundnut seed samples were performed using ultra performance liquid chromatography; 22.5% and 41.3% of samples were positive, with total aflatoxin concentrations of 786 and 3135 ng g⁻¹ from 2013/2014 and 2014/2015 …

Draft Genome Sequence Of Cercospora Arachidicola, Causal Agent Of Early Leaf Spot In Peanuts, Valerie A. Orner, Emily G. Cantowine, Xinye Monica Wang, Amr Abouelleil, James Bochicchio, Chad Nusbaum, Albert C. Culbreath, Zaid Abdo, Renee S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cercospora arachidicola, causal agent of early leaf spot, is an economically important peanut pathogen. Lack of genetic information about this fungus prevents understanding the role that potentially diverse genotypes may have in peanut breeding programs. Here, we report for the first time a draft genome sequence of C. arachidicola.

Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in …