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Full-Text Articles in Agronomy and Crop Sciences
Influence Of Six Herbicides Applied To Silage Corn On Fall Planted Rye And Radish Cover Crop Growth In South Dakota Soils, Sydney Pridie
Influence Of Six Herbicides Applied To Silage Corn On Fall Planted Rye And Radish Cover Crop Growth In South Dakota Soils, Sydney Pridie
The Journal of Undergraduate Research
Spring preemergence herbicide applications are often used for burndown of existing weeds and residual control, eliminating weed presence during early season crop growth. There has been an increase in the interest in planting cover crops soon after cash crop removal, due to the potential soil and production benefits. However, soil herbicide residuals may result in poor cover crop growth. This study examined the growth of radish (Raphanus sativa) and rye (Secale cereal), species often used as cover crops, in soils that had been treated with residual herbicides about 100 d prior to cover crop planting. The …
Evaluating Physiological Responses Of Ten Alfalfa (Medicago Sativa Subsp. Falcata) Germplasm To Drought Treatments, Austin Hanson
Evaluating Physiological Responses Of Ten Alfalfa (Medicago Sativa Subsp. Falcata) Germplasm To Drought Treatments, Austin Hanson
The Journal of Undergraduate Research
Alfalfa is the most widely produced perennial forage legume in North America. However, its use in the semiarid northern Great Plains is limited due to poor stand establishment and persistence under drought condition. The development of drought-tolerant alfalfa cultivars is of great need. Some Medicago sativa subsp. falcata populations have demonstrated promising drought resistance when compared to M. sativa. Morphological and physiological mechanisms play a critical role in drought tolerance by influencing seedling survival, stand establishment and drought recovery. Assessment of variability in physiological responses to drought among germplasm lines to drought is necessary for developing cultivars with improved drought …
Identifying Promising New Falcata Alfalfa Populations For Use In Semiarid Rangelands, Derek Kannenberg
Identifying Promising New Falcata Alfalfa Populations For Use In Semiarid Rangelands, Derek Kannenberg
The Journal of Undergraduate Research
Alfalfa (Medicago sativa L.) is a valuable crop worldwide. In addition to its use as forage, the nitrogen fixation capabilities of root nodules make it a valuable component for improving soil health. Unfortunately, the semi-arid region of the Northern Great Plains introduces a number of environmental stresses that are detrimental to stand establishment and persistence. The ability to produce adventitious shoots from roots is generally considered a favorable trait for increasing stress resistance. This study aimed to identify alfalfa populations possessing the ability to produce adventitious shoot from root segments. Seven Plant Introductions (PIs), selected from the National Plant …
Selection Of Winter Wheat Mutant Lines Resistant To Drought Stress, Brian Kontz, Sean Franklin, Charles Brunel
Selection Of Winter Wheat Mutant Lines Resistant To Drought Stress, Brian Kontz, Sean Franklin, Charles Brunel
The Journal of Undergraduate Research
Mutated winter wheat lines were screened for improved drought resistance compared to the wildtype by withholding water for 3 weeks after 9 weeks of normal growth, and noting their responses upon re-watering. Of the 146 mutants grown, 73% were either killed due to water stress or showed poor recovery, while 27% recovered better than the wildtype line. This experiment enabled the selection of candidates for further investigation to better understand the genetic basis of drought tolerance in winter wheat.
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
The Journal of Undergraduate Research
Numerous protocols are available for the isolation of plant genomic DNA. Often times, these protocols utilize a wide variety of solutions. The primary purpose of this project was to examine the use of LiCl for the removal of RNA contaminants within genomic DNA samples and its dependency on incubation time and temperature. Our results indicate that LiCl is sufficient for the removal of high molecular weight RNA contaminants from genomic DNA. In addition, our results illustrate varying incubation times with LiCl yield minimal differences in the recovery of genomic DNA and the removal of RNA contaminants. Alternatively, different incubation temperatures …
The Fate If 2, 4-D In Intact Soybean (Glycine Mix), Laura Hummel, Anthony Mayer
The Fate If 2, 4-D In Intact Soybean (Glycine Mix), Laura Hummel, Anthony Mayer
The Journal of Undergraduate Research
About 479,000 pounds of 2,4-D (2, 4-dichlorophenoxyacetic acid) was applied to South Dakota's crops in 2000. 2,4-D can injure or reduce yield of soybean (Glycine max) if deposited on growing plants. This study determined uptake, translocation, and metabolism of ring-labeled-2,4-D in soybean at the third trifoliate (V3) stage of growth. Plants were harvested and partitioned into four parts from 1 hr (HAT)to 10 d (DAT) after treatment. Thin layer chromatography techniques were used to determine if remaining in the tissue was parent chemical or metabolite. 2,4-D uptake ranged from 39% at 1 HAT to 74% 6 DAT.By 10 DAT, translocated …
Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman
Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman
The Journal of Undergraduate Research
HVCR21 is a barley protein known to be cold regulated at the mRNA level. However, its function as well as the affect of low temperature on its translation are unknown. The purpose of this project was to engineer E. coli to express recombinant HVCR21. PGR primers were designed for the 5' and 3' ends of the HVCR21 coding region. The primers were also designed with a 5' SacI restriction site and a 3' Pst I restriction site. After ligation of the PCR product into the pCR4-TOPO vector, bacteria were transformed and plated and the successful transformant verified by PCR. The …