Agronomy and Crop Sciences Commons^™

Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is the staple food for a majority of the world’s population, and uses 30% of the global fresh water during its life cycle. Drought at the reproductive stage is the most important abiotic stress factor limiting grain yield. The United States is the third largest exporter of rice, and Arkansas is the top rice-producing state. The Arkansas rice-growing region in the Lower Mississippi belt is among the 10 areas with the highest risk of water scarcity. Adapted U.S. rice cultivars were screened for drought resistant (DR) traits to find sources for breeding U.S. rice cultivars for …

Distribution Of Genomic Variation In The Usda Soybean Germplasm Collection And Relationship With Phenotypic Variation, Nonoy Batiller Bandillo

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The USDA Soybean Germplasm Collection harbors a large stock of genetic diversity with potential to accelerate soybean cultivar development. The extent and nature of favorable alleles contained in the collection are not well known nor is the distribution of genetic variation and how it relates to phenotypic variation. The genotyping of the entire USDA Soybean Germplasm Collection marked the beginning of a systematic exploration of genetic diversity for genetic research and breeding. In this research, we conducted the first comprehensive analysis of population structure on the collection of ~14,400 soybean accessions [Glycine max (L.) Merr. and G. soja Siebold …

Functional Genomics Of Maize Endosperm Maturation And Protein Quality, Lingling Yuan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Maize is one of the most important cereal crops and widely cultivated throughout the world. The study on maize kernel development including protein quality improvement is essential for removing dietary protein deficiency because of the lack of essential amino acids, especially lysine and tryptophan, in maize kernel. Quality Protein Maize (QPM) is a hard kernel variant of the high-lysine mutant, opaque-2. We created opaque QPM variants to identify opaque-2 modifier genes and to investigate deletion mutagenesis combined with Illumina sequencing as a maize functional genomics tool. A K0326Y-QPM deletion mutant, line 107, was null for the 27- and 50-kD …

Crop Updates 2003 - Cereals, Graham Crosbie, Robert Loughman, Collin Wellings, Greg Shea, Simon Mckirdy, Neil C. Turner, Brenda Shackley, Wal Anderson, Darshan Sharma, Mohammad Amjad, Steve Penny Jr, Melanie Kupsch, Anne Smith, Veronika Reck, Pam Burgess, Glenda Smith, Elizabeth Tierney, Peter Burges, Moin Salam, Megan Collins, Art Diggle, Blakely Paynter, Roslyn Jetter, Kevin Young, Jocelyn Ball, Natasha Littlewood, Lucy Anderton, Irene Waters, Tim Setter, Jeff Russell, Reg Lance, Chengdao Li, Sue Broughton, Michael Jones, Grace Zawko, Keith Gregg, Stephen Loss, Frank Ripper, Ryan Guthrie, Daniel Bell, Patrick Gethin, Narelle Hill, Laurence Caeslake, Vivien Vanstone, Sean Kelly, Helen Hunter, Christopher R. Newman

Crop Updates

This session covers twenty one papers from different authors:

PLENARY

1. Recognising and responding to new market opportunities in the grains industry, Graham Crosbie, Manager, Grain Products Research, Crop Breeding, Plant Industries, Department of Agriculture

2. Stripe rust – where to now for the WA wheat industry? Robert Loughman¹, Colin Wellings² and Greg Shea¹¹Department of Agriculture, ²University of Sydney Plant Breeding Institute, Cobbitty (on secondment from NSW Agriculture)

3. Benefits of a Grains Biosecurity Plan, Dr Simon McKirdy, Plant Health Australia, Mr Greg Shea, Department of Agriculture

4. Can we improve …

Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …