Agronomy and Crop Sciences Commons^™

Characterization Of Genetic Sources Associated With Restorability And Seed Dimension In Arkansas Restorer Rice Lines, Ozgur Azapoglu

Graduate Theses and Dissertations

Hybrid rice (Oryza sativa L.) breeding offers a significant opportunity to enhance rice production, and the cultivation of a male sterile line is the most important factor in the success of cross-breeding. One of the key elements of hybrid rice production is to develop a restorer line that is assigned as the male parent. The restorer lines provide viable pollen for fertilization of the male sterile plant due to the presence of a restorer gene (Rf) in their genomes. Any superior restorer line applied to hybrid rice production must contain genes/quantitative trait loci (QTL) associated with the desirable agronomic traits …

Improving Breeding Program Efficiency And Genetic Gain Through The Implementation Of Genomic Selection In Diverse Wheat Germplasm, Dylan Larkin

Graduate Theses and Dissertations

Genomic selection (GS) is an important tool for increasing genetic gain for economically important traits in breeding programs. Genomic selection uses molecular markers across the entire genome in order to predict the performance of breeding lines for a trait of interest prior to phenotyping. A training population (TP) of elite germplasm, representative of the University of Arkansas wheat breeding program, was developed in order to predict important agronomic and Fusarium head blight (FHB) resistance traits within the University of Arkansas wheat breeding program through cross-validation and forward prediction.

A genome-wide association study (GWAS) was performed on the TP to identify …

Resistance To Herbicides Conferred By Amaranthus Palmeri Protoporphyrinogen Ix Oxidase Mutations, Pamela Carvalho De Lima

Graduate Theses and Dissertations

Palmer amaranth (Amaranthus palmeri S. Wats.) is one of the most problematic agronomic weeds to control in fields across Arkansas. Thus far, this species has evolved resistance to several herbicides, including protoporphyrinogen IX oxidase (PPO) inhibitors. The majority of PPO-resistant Palmer amaranth populations harbor a target-site mutation (substitution or deletion of amino acids). The objective of this thesis was to identify the level of fomesafen resistance conferred by PPO2 mutations from Palmer amaranth. The experiments conducted aimed to (1) characterize the level of resistance conferred by the transgene Palmer amaranth ppo2 carrying ΔG210 mutation into the wild type rice (Oryza …

Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is the staple food for a majority of the world’s population, and uses 30% of the global fresh water during its life cycle. Drought at the reproductive stage is the most important abiotic stress factor limiting grain yield. The United States is the third largest exporter of rice, and Arkansas is the top rice-producing state. The Arkansas rice-growing region in the Lower Mississippi belt is among the 10 areas with the highest risk of water scarcity. Adapted U.S. rice cultivars were screened for drought resistant (DR) traits to find sources for breeding U.S. rice cultivars for …

Association Mapping For Soybean (Glycine Max L. Merr.) Protein And Oil Content, Joseph Najjar

Graduate Theses and Dissertations

Soybean [Glycine Max (L.) Merrill] is an important economic crop because of its high content of seed protein and oil. Seed oil and protein content are quantitative inherited traits. The genetics of seed protein and oil levels have been extensively studied, with 367 QTL reported for protein and 475 QTL reported for oil to date. Validation of such QTLs, and identification of easily-automatable molecular markers around these QTL will aid the progression of breeding for such traits. The focus of this research was to discover novel and verify previously reported QTL related to protein and/or oil content via Genome-Wide Association …

Phenotypic And Genotypic Studies In The Peach [Prunus Persica (L.) Batsch] And Muscadine Grape (Vitis Rotundifolia Michx.), Jennifer Anne Lewter

Graduate Theses and Dissertations

Peach: Peaches [Prunus persica (L.) Batsch] are routinely chilled to increase shelf-life. Exposure to temperatures of 5° C for two weeks can induce chilling injury (CI) symptoms, including flesh mealiness (or wooliness) and a lack of juiciness. Phenotypic data were collected on seven biparental F1 peach populations maintained at the University of Arkansas Fruit Research Station. A genome wide association study (GWAS) was performed using TASSEL 5 which identified four quantitative trait loci (QTLs) associated with expressible juice, four QTLs for mealiness, five QTLs for soluble solids, and three QTLs for fruit weight. Exploiting these genetic markers could help breeders …

Genetics Of Physiological Traits Associated With Drought Tolerance In Soybean (Glycine Max), Sumandeep Kaur Bazzer

Graduate Theses and Dissertations

Soybean (Glycine max L.) is one of the major row crops in the United States, and its production is often limited by drought stress. Physiological traits from exotic germplasm that confer drought tolerance may be useful in improving commercial soybean production. For example, carbon isotope ratio (δ13C) is positively correlated with water use efficiency (WUE), and nitrogen isotope ratio (δ15N) is negatively correlated with N2 fixation; canopy temperature (CT) is an indicator for genetic variation in transpiration and stomatal conductance. Therefore, the objectives of this research were to identify the genomic regions associated with: (1) δ13C and δ15N using a …

Developing A High-Throughput Method To Screen Soybean Germplasm For Hypoxia Tolerance In Hydroponic Systems, Derrick Harrison

Graduate Theses and Dissertations

Soybean [Glycine max (Merr.)], the second most planted crop in the United States, is sensitive to field flooding due to depletion of oxygen and accumulation of CO2 in the rhizosphere. There is a need to breed cultivars that are adapted to areas prone to flooding, but field evaluations in the U.S. are limited because of time (one generation per year) and availability of resources (impermeable soils, irrigation, equipment to build levees). The purpose of this study was to develop and execute a protocol of germplasm screening for hypoxia tolerance using hydroponics in a controlled greenhouse environment. Germination rates and vigor …

An Evaluation Of Unmanned Aircraft Systems' Ability To Assess Stripe Rust In Large Wheat Breeding Nursies, Jamison T. Murry

Graduate Theses and Dissertations

Stripe Rust (Puccinia striiformis f. sp. tritici) is a foliar disease that significantly impacts global wheat production, and resistant cultivars provide the most efficient method of control. High-throughput phenotyping using unmanned aircraft systems (UAS) offers a potentially more efficient method for field-based phenotyping compared to visual assessment. Here we tested the ability of remote sensing to predict stripe rust severity in a diverse population of 594 soft red winter wheat lines, planted in single-rows, and evaluated them by visually rating stripe rust intensity and remotely using the dark green color index (DGCI), normalized difference vegetation index (NDVI) and blue NDVI. …

Determination Of Critical Y-Leaf Potassium Concentrations Across Reproductive Growth Stages For Direct-Seeded Delay-Flooded Rice, Chandler Edwin Gruener

Graduate Theses and Dissertations

Rice (Oryza sativa L.) grain yield can be limited by potassium (K) deficiency on soils low in exchangeable K. Visually diagnosing K deficiency during early reproductive growth is not easily done and the interpretation of traditional tissue analysis is limited to select growth-stages. Our primary focus was to define continuous critical Y-leaf-K concentrations during reproductive growth for the production of maximal grain yield. A secondary objective was to examine Y-leaf sap-K concentration, measured using a handheld device, as a rapid in-field method of monitoring rice plant K nutrition. The Y-leaf is defined as the uppermost fully extended leaf with a …