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Generation And Evaluation Of Modified Opaque-2 (O2) Popcorn Suggests A Route To Quality Protein Popcorn (Qpp), Ying Ren Dec 2018

Generation And Evaluation Of Modified Opaque-2 (O2) Popcorn Suggests A Route To Quality Protein Popcorn (Qpp), Ying Ren

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

I have been working on a Quality Protein Popcorn breeding project where QPM conversion is carried out simultaneously for several elite popcorn germplasms. During my study in the graduate program, I led the following aspects of the Quality Protein Popcorn Breeding Project:

1. Identified suitable QPMs as opaque-2 allele donors.

2.Examined the feasibility of quick introgression of the opaque-2 allele into popcorn lines via marker-assisted selection.

3. Monitored modification by SDS-PAGE zein profiling and light box phenotypic selection to make sure multiple modifier loci for opaque-2 were incorporated each time generation advancement was carried out.

4. Carried out high throughput …