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Full-Text Articles in Agronomy and Crop Sciences
Identifying Promising New Falcata Alfalfa Populations For Use In Semiarid Rangelands, Derek Kannenberg
Identifying Promising New Falcata Alfalfa Populations For Use In Semiarid Rangelands, Derek Kannenberg
The Journal of Undergraduate Research
Alfalfa (Medicago sativa L.) is a valuable crop worldwide. In addition to its use as forage, the nitrogen fixation capabilities of root nodules make it a valuable component for improving soil health. Unfortunately, the semi-arid region of the Northern Great Plains introduces a number of environmental stresses that are detrimental to stand establishment and persistence. The ability to produce adventitious shoots from roots is generally considered a favorable trait for increasing stress resistance. This study aimed to identify alfalfa populations possessing the ability to produce adventitious shoot from root segments. Seven Plant Introductions (PIs), selected from the National Plant …
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
The Journal of Undergraduate Research
Numerous protocols are available for the isolation of plant genomic DNA. Often times, these protocols utilize a wide variety of solutions. The primary purpose of this project was to examine the use of LiCl for the removal of RNA contaminants within genomic DNA samples and its dependency on incubation time and temperature. Our results indicate that LiCl is sufficient for the removal of high molecular weight RNA contaminants from genomic DNA. In addition, our results illustrate varying incubation times with LiCl yield minimal differences in the recovery of genomic DNA and the removal of RNA contaminants. Alternatively, different incubation temperatures …